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Literature summary extracted from

  • Chiang, C.; Bennett, R.E.
    Purification and properties of penicillin amidase from Bacillus megaterium (1967), J. Bacteriol., 93, 302-308.
    View publication on PubMedView publication on EuropePMC

Application

EC Number Application Comment Organism
3.5.1.11 synthesis production of 6-aminopenicillanate as precursor for the production of semi-synthetic beta-lactam derivatives Priestia megaterium

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.5.1.11 6-aminopenicillanate product inhibition Priestia megaterium
3.5.1.11 Co2+
-
Priestia megaterium
3.5.1.11 Fe2+
-
Priestia megaterium
3.5.1.11 Mn2+
-
Priestia megaterium
3.5.1.11 additional information no inhibition with sulfhydryl reagents Priestia megaterium
3.5.1.11 Ni2+
-
Priestia megaterium
3.5.1.11 phenylacetic acid product inhibition, competitve Priestia megaterium

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.5.1.11 4.5
-
benzylpenicillin 37°C, pH 8.7 Priestia megaterium

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.5.1.11 extracellular
-
Priestia megaterium
-
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.5.1.11 additional information no stimulation with divalent metal ions Priestia megaterium

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.5.1.11 120000
-
sedimentation equilibrium centrifugation Priestia megaterium

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.5.1.11 benzylpenicillin + H2O Priestia megaterium highly specific phenylacetic acid + 6-aminopenicillanate
-
?
3.5.1.11 benzylpenicillin + H2O Priestia megaterium 14945 highly specific phenylacetic acid + 6-aminopenicillanate
-
?

Organism

EC Number Organism UniProt Comment Textmining
3.5.1.11 Priestia megaterium
-
-
-
3.5.1.11 Priestia megaterium 14945
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.5.1.11
-
Priestia megaterium

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.5.1.11 31.7
-
substrate benzylpenicillin, purified enzyme, 37°C, pH 8.7 Priestia megaterium

Storage Stability

EC Number Storage Stability Organism
3.5.1.11 4°C, 0.05 M phosphate buffer, pH 7.0, purified enzyme, stable for at least 3 months Priestia megaterium

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.5.1.11 allylmercaptomethylpenicillin + H2O poor substrate Priestia megaterium ?
-
?
3.5.1.11 benzylpenicillin + H2O highly specific Priestia megaterium phenylacetic acid + 6-aminopenicillanate
-
?
3.5.1.11 benzylpenicillin + H2O complete hydrolysis at low substrate concentrations Priestia megaterium phenylacetic acid + 6-aminopenicillanate
-
?
3.5.1.11 benzylpenicillin + H2O highly specific Priestia megaterium 14945 phenylacetic acid + 6-aminopenicillanate
-
?
3.5.1.11 benzylpenicillin + H2O complete hydrolysis at low substrate concentrations Priestia megaterium 14945 phenylacetic acid + 6-aminopenicillanate
-
?
3.5.1.11 beta-(alpha-toluylamido)-propionic acid + H2O poor substrate Priestia megaterium ?
-
?
3.5.1.11 D,L-N-phenylacetylalanine + H2O poor substrate Priestia megaterium ?
-
?
3.5.1.11 delta2-pentenylpenicillin + H2O poor substrate Priestia megaterium ?
-
?
3.5.1.11 ethylthiomethylpenicillin + H2O poor substrate Priestia megaterium ?
-
?
3.5.1.11 additional information no activity with n-heptylpenicillin, 2-(phenylthio)-ethylpenicillin, dimethoxyphenylpenicillin, and 5-methyl-3-phenyl-4-isoxazolylpenicillin Priestia megaterium ?
-
?
3.5.1.11 additional information no activity with n-heptylpenicillin, 2-(phenylthio)-ethylpenicillin, dimethoxyphenylpenicillin, and 5-methyl-3-phenyl-4-isoxazolylpenicillin Priestia megaterium 14945 ?
-
?
3.5.1.11 N-methylphenylacetamide + H2O poor substrate Priestia megaterium ?
-
?
3.5.1.11 penicillin V + H2O poor substrate Priestia megaterium 6-aminopenicillanic acid + phenoxyacetic acid
-
?
3.5.1.11 penicillin V + H2O poor substrate Priestia megaterium 14945 6-aminopenicillanic acid + phenoxyacetic acid
-
?
3.5.1.11 phenoxyethylpenicillin + H2O poor substrate Priestia megaterium 3-phenoxypropanoic acid + 6-aminopenicillanic acid
-
?
3.5.1.11 phenylacetamide + H2O poor substrate Priestia megaterium ?
-
?
3.5.1.11 phenylacetamide + H2O poor substrate Priestia megaterium 14945 ?
-
?
3.5.1.11 phenylacetamide + H2O poor substrate Priestia megaterium NH3 + ?
-
?

Synonyms

EC Number Synonyms Comment Organism
3.5.1.11 penicillin G acylase
-
Priestia megaterium
3.5.1.11 PGA
-
Priestia megaterium

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.5.1.11 45
-
-
Priestia megaterium

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.5.1.11 -20
-
pH 8.4, protein concentration of 1 mg/ml, stable for at least 8 hours Priestia megaterium
3.5.1.11 5
-
pH 8.4, protein concentration of 1 mg/ml, stable for at least 8 hours Priestia megaterium
3.5.1.11 24
-
pH 8.4, protein concentration of 1 mg/ml, stable for at least 8 hours Priestia megaterium
3.5.1.11 37
-
pH 8.4, protein concentration of 1 mg/ml, stable for at least 8 hours Priestia megaterium
3.5.1.11 45
-
pH 8.4, protein concentration of 1 mg/ml, 70% of activity lost within 8 hours Priestia megaterium
3.5.1.11 60
-
pH 8.4, protein concentration of 1 mg/ml, complete denaturation within 8 hours Priestia megaterium

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.5.1.11 8 9
-
Priestia megaterium

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.5.1.11 7.5 9.5 rapid decrease in activity when the pH is raised above 9.5 or lowered below 7.5 Priestia megaterium

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
3.5.1.11 6.5 10 highest stability when incubated at 24°C for 9 h Priestia megaterium

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
3.5.1.11 26
-
6-aminopenicillanate 37°C, pH 8.7 Priestia megaterium
3.5.1.11 450
-
phenylacetic acid 37°C, pH 8.7 Priestia megaterium