EC Number | Application | Comment | Organism |
---|---|---|---|
3.5.1.11 | synthesis | production of 6-aminopenicillanate as precursor for the production of semi-synthetic beta-lactam derivatives | Priestia megaterium |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
3.5.1.11 | 6-aminopenicillanate | product inhibition | Priestia megaterium | |
3.5.1.11 | Co2+ | - |
Priestia megaterium | |
3.5.1.11 | Fe2+ | - |
Priestia megaterium | |
3.5.1.11 | Mn2+ | - |
Priestia megaterium | |
3.5.1.11 | additional information | no inhibition with sulfhydryl reagents | Priestia megaterium | |
3.5.1.11 | Ni2+ | - |
Priestia megaterium | |
3.5.1.11 | phenylacetic acid | product inhibition, competitve | Priestia megaterium |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
3.5.1.11 | 4.5 | - |
benzylpenicillin | 37°C, pH 8.7 | Priestia megaterium |
EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|---|
3.5.1.11 | extracellular | - |
Priestia megaterium | - |
- |
EC Number | Metals/Ions | Comment | Organism | Structure |
---|---|---|---|---|
3.5.1.11 | additional information | no stimulation with divalent metal ions | Priestia megaterium |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
3.5.1.11 | 120000 | - |
sedimentation equilibrium centrifugation | Priestia megaterium |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
3.5.1.11 | benzylpenicillin + H2O | Priestia megaterium | highly specific | phenylacetic acid + 6-aminopenicillanate | - |
? | |
3.5.1.11 | benzylpenicillin + H2O | Priestia megaterium 14945 | highly specific | phenylacetic acid + 6-aminopenicillanate | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
3.5.1.11 | Priestia megaterium | - |
- |
- |
3.5.1.11 | Priestia megaterium 14945 | - |
- |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
3.5.1.11 | - |
Priestia megaterium |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
3.5.1.11 | 31.7 | - |
substrate benzylpenicillin, purified enzyme, 37°C, pH 8.7 | Priestia megaterium |
EC Number | Storage Stability | Organism |
---|---|---|
3.5.1.11 | 4°C, 0.05 M phosphate buffer, pH 7.0, purified enzyme, stable for at least 3 months | Priestia megaterium |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
3.5.1.11 | allylmercaptomethylpenicillin + H2O | poor substrate | Priestia megaterium | ? | - |
? | |
3.5.1.11 | benzylpenicillin + H2O | highly specific | Priestia megaterium | phenylacetic acid + 6-aminopenicillanate | - |
? | |
3.5.1.11 | benzylpenicillin + H2O | complete hydrolysis at low substrate concentrations | Priestia megaterium | phenylacetic acid + 6-aminopenicillanate | - |
? | |
3.5.1.11 | benzylpenicillin + H2O | highly specific | Priestia megaterium 14945 | phenylacetic acid + 6-aminopenicillanate | - |
? | |
3.5.1.11 | benzylpenicillin + H2O | complete hydrolysis at low substrate concentrations | Priestia megaterium 14945 | phenylacetic acid + 6-aminopenicillanate | - |
? | |
3.5.1.11 | beta-(alpha-toluylamido)-propionic acid + H2O | poor substrate | Priestia megaterium | ? | - |
? | |
3.5.1.11 | D,L-N-phenylacetylalanine + H2O | poor substrate | Priestia megaterium | ? | - |
? | |
3.5.1.11 | delta2-pentenylpenicillin + H2O | poor substrate | Priestia megaterium | ? | - |
? | |
3.5.1.11 | ethylthiomethylpenicillin + H2O | poor substrate | Priestia megaterium | ? | - |
? | |
3.5.1.11 | additional information | no activity with n-heptylpenicillin, 2-(phenylthio)-ethylpenicillin, dimethoxyphenylpenicillin, and 5-methyl-3-phenyl-4-isoxazolylpenicillin | Priestia megaterium | ? | - |
? | |
3.5.1.11 | additional information | no activity with n-heptylpenicillin, 2-(phenylthio)-ethylpenicillin, dimethoxyphenylpenicillin, and 5-methyl-3-phenyl-4-isoxazolylpenicillin | Priestia megaterium 14945 | ? | - |
? | |
3.5.1.11 | N-methylphenylacetamide + H2O | poor substrate | Priestia megaterium | ? | - |
? | |
3.5.1.11 | penicillin V + H2O | poor substrate | Priestia megaterium | 6-aminopenicillanic acid + phenoxyacetic acid | - |
? | |
3.5.1.11 | penicillin V + H2O | poor substrate | Priestia megaterium 14945 | 6-aminopenicillanic acid + phenoxyacetic acid | - |
? | |
3.5.1.11 | phenoxyethylpenicillin + H2O | poor substrate | Priestia megaterium | 3-phenoxypropanoic acid + 6-aminopenicillanic acid | - |
? | |
3.5.1.11 | phenylacetamide + H2O | poor substrate | Priestia megaterium | ? | - |
? | |
3.5.1.11 | phenylacetamide + H2O | poor substrate | Priestia megaterium 14945 | ? | - |
? | |
3.5.1.11 | phenylacetamide + H2O | poor substrate | Priestia megaterium | NH3 + ? | - |
? |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
3.5.1.11 | penicillin G acylase | - |
Priestia megaterium |
3.5.1.11 | PGA | - |
Priestia megaterium |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
3.5.1.11 | 45 | - |
- |
Priestia megaterium |
EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|---|
3.5.1.11 | -20 | - |
pH 8.4, protein concentration of 1 mg/ml, stable for at least 8 hours | Priestia megaterium |
3.5.1.11 | 5 | - |
pH 8.4, protein concentration of 1 mg/ml, stable for at least 8 hours | Priestia megaterium |
3.5.1.11 | 24 | - |
pH 8.4, protein concentration of 1 mg/ml, stable for at least 8 hours | Priestia megaterium |
3.5.1.11 | 37 | - |
pH 8.4, protein concentration of 1 mg/ml, stable for at least 8 hours | Priestia megaterium |
3.5.1.11 | 45 | - |
pH 8.4, protein concentration of 1 mg/ml, 70% of activity lost within 8 hours | Priestia megaterium |
3.5.1.11 | 60 | - |
pH 8.4, protein concentration of 1 mg/ml, complete denaturation within 8 hours | Priestia megaterium |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
3.5.1.11 | 8 | 9 | - |
Priestia megaterium |
EC Number | pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|---|
3.5.1.11 | 7.5 | 9.5 | rapid decrease in activity when the pH is raised above 9.5 or lowered below 7.5 | Priestia megaterium |
EC Number | pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|---|
3.5.1.11 | 6.5 | 10 | highest stability when incubated at 24°C for 9 h | Priestia megaterium |
EC Number | Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|---|
3.5.1.11 | 26 | - |
6-aminopenicillanate | 37°C, pH 8.7 | Priestia megaterium | |
3.5.1.11 | 450 | - |
phenylacetic acid | 37°C, pH 8.7 | Priestia megaterium |