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Literature summary extracted from

  • Drevland, R.M.; Waheed, A.; Graham, D.E.
    Enzymology and evolution of the pyruvate pathway to 2-oxobutyrate in Methanocaldococcus jannaschii (2007), J. Bacteriol., 189, 4391-4400.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.1.1.85 expressed in Escherichia coli as a His-tagged fusion protein Methanocaldococcus jannaschii

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.1.85 0.024
-
 (2R,3S)-3-isopropylmalate kcat/Km: 88000/Msec Methanocaldococcus jannaschii
1.1.1.85 0.41
-
 D-malate kcat/Km: 14000/Msec Methanocaldococcus jannaschii

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.1.1.85 40000
-
 SDS-PAGE Methanocaldococcus jannaschii

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.85 Methanocaldococcus jannaschii
-
-
-
4.1.3.22 Methanocaldococcus jannaschii
-
-
-
4.2.1.36 Methanocaldococcus jannaschii
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.1.1.85 purified by heat treatment followed by nickel affinity chromatography Methanocaldococcus jannaschii

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.85 (2R,3S)-3-isopropylmalate + NAD+
-
 Methanocaldococcus jannaschii 2-ketoisocaproate + NADH + H+ + CO2
-
 ?
1.1.1.85 beta-methylmalate + NAD+ enzyme catalyzes the final step in the pyruvate pathway to 2-oxobutyrate Methanocaldococcus jannaschii 2-oxobutyrate + NADH + CO2
-
 ?
1.1.1.85 D-malate + NAD+
-
 Methanocaldococcus jannaschii pyruvate + NADH + CO2
-
 ?
1.1.1.85 additional information enzyme does not catalyze the oxidation of L-malate, L-tartrate, D-tartrate, DL-isocitrate or DL-lactate Methanocaldococcus jannaschii ?
-
 ?
4.1.3.22 acetate + pyruvate
-
 Methanocaldococcus jannaschii citramalate
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.1.1.85 IPMDH
-
 Methanocaldococcus jannaschii
1.1.1.85 isopropylmalate dehydrogenase
-
 Methanocaldococcus jannaschii
4.1.3.22 citramalate synthase
-
 Methanocaldococcus jannaschii
4.1.3.22 CMS
-
 Methanocaldococcus jannaschii
4.2.1.36 HACN
-
 Methanocaldococcus jannaschii