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Literature summary extracted from

  • Fernandes, C.; Empadinhas, N.; da Costa, M.S.
    Single-step pathway for synthesis of glucosylglycerate in Persephonella marina (2007), J. Bacteriol., 189, 4014-4019.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.4.1.266 gene gpgS, DNA and amino acid sequence determination and analysis, expression in Escherichia coli strain BL21(DE3), the genes for glucosyl-3-phosphoglycerate synthase, GpgS, and glucosyl-3-phosphoglycerate phosphatase, GpgP, the enzymes that lead to the synthesis of glucosylglycerol through the formation of glucosyl-3-phosphoglycerate, are organized in anoperon-like structure with the gene encoding a putative glycosyltransferase, the glucosylglycerate synthase, Ggs, overview Persephonella marina
2.4.1.268 expression in Escherichia coli Persephonella marina

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.4.1.268 EDTA
-
 Persephonella marina

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.4.1.266 additional information
-
 additional information kinetics, recombinant enzyme Persephonella marina
2.4.1.268 1.5
-
 ADP-glucose pH 6.5, 70°C Persephonella marina
2.4.1.268 2.2
-
 D-glycerate pH 6.5, 70°C Persephonella marina
2.4.1.268 6.8
-
 UDP-glucose pH 6.5, 70°C Persephonella marina
2.4.1.268 7.1
-
 GDP-glucose pH 6.5, 70°C Persephonella marina

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.4.1.266 Mg2+
-
 Persephonella marina
2.4.1.268 Ca2+ 20 mM, strongly stimulates glucosylglycerate synthase activity Persephonella marina
2.4.1.268 Mg2+ 20 mM, strongly stimulates glucosylglycerate synthase activity Persephonella marina
2.4.1.268 additional information no stimulation by Ba2+, Co2+, Fe3+, Li+, Mn2+, Sr2+ or Zn2+ Persephonella marina

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.4.1.268 48000
-
 4 * 48000, SDS-PAGE Persephonella marina
2.4.1.268 48400
-
 4 * 48400, calculated from sequence Persephonella marina
2.4.1.268 210000
-
 gel filtration Persephonella marina

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.4.1.266 additional information Persephonella marina the genes for glucosyl-3-phosphoglycerate synthase, GpgS, and glucosyl-3-phosphoglycerate phosphatase, GpgP, the enzymes that lead to the synthesis of glucosylglycerol through the formation of glucosyl-3-phosphoglycerate, are organized in anoperon-like structure with the gene encoding a putative glycosyltransferase, the glucosylglycerate synthase, Ggs, overview, biosynthetic pathway overview ?
-
 ?
2.4.1.268 ADP-glucose + glycerate Persephonella marina in Persephonella marina two pathways for synthesis of glucosylglycerate are present: 1. the single-step pathway in with glucosylglycerate synthase (Ggs) catalyzes the synthesis of 2-(alpha-D-glucosyl)-D-glycerate in one-step from ADP-glucose and D-glycerate, and 2. the two-step pathway in which glucosyl-3-phosphoglycerate synthase (GpgS) catalyzes the conversion of NDP-glucose and D-3-phosphoglycerate into glucosyl-3-phosphoglycerate, which is then converted to 2-(alpha-D-glucopyranosyl)-D-glycerate by glucosyl-3-phosphoglycerate phosphatase (GpgP) 2-O-(alpha-D-glucopyranosyl)-D-glycerate + ADP
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.4.1.266 Persephonella marina A9QXB4 strain DSM 14350
-
2.4.1.268 Persephonella marina
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.4.1.268
-
 Persephonella marina

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.4.1.268 27.4
-
 pH 6.5, 70°C, substrate: GDP-glucose Persephonella marina
2.4.1.268 47
-
 pH 6.5, 70°C, substrate: UDP-glucose Persephonella marina
2.4.1.268 284.3
-
 pH 6.5, 70°C, substrate: ADP-glucose Persephonella marina

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.4.1.266 ADP-alpha-D-glucose + D-3-phosphoglycerate
-
 Persephonella marina ADP + 2-O-(alpha-D-glucopyranosyl)-3-phospho-D-glycerate
-
 ?
2.4.1.266 GDP-glucose + D-3-phosphoglycerate
-
 Persephonella marina GDP + 2-(beta-D-glucosyl)-sn-glycerol 3-phosphate
-
 ?
2.4.1.266 additional information the genes for glucosyl-3-phosphoglycerate synthase, GpgS, and glucosyl-3-phosphoglycerate phosphatase, GpgP, the enzymes that lead to the synthesis of glucosylglycerol through the formation of glucosyl-3-phosphoglycerate, are organized in anoperon-like structure with the gene encoding a putative glycosyltransferase, the glucosylglycerate synthase, Ggs, overview, biosynthetic pathway overview Persephonella marina ?
-
 ?
2.4.1.266 UDP-glucose + D-3-phosphoglycerate
-
 Persephonella marina UDP + 2-(beta-D-glucosyl)-sn-glycerol 3-phosphate
-
 ?
2.4.1.268 ADP-glucose + D-glycerate ADP-glucose is the preferred substrate (0.2843 mol/min*mg), it can be partially replaced by UDP-glucose or GDP-glucose in decreasing order of efficiency (0.047 and 0.0274 mol/min*mg, respectively) Persephonella marina 2-O-(alpha-D-glucopyranosyl)-D-glycerate + ADP
-
 ?
2.4.1.268 ADP-glucose + glycerate in Persephonella marina two pathways for synthesis of glucosylglycerate are present: 1. the single-step pathway in with glucosylglycerate synthase (Ggs) catalyzes the synthesis of 2-(alpha-D-glucosyl)-D-glycerate in one-step from ADP-glucose and D-glycerate, and 2. the two-step pathway in which glucosyl-3-phosphoglycerate synthase (GpgS) catalyzes the conversion of NDP-glucose and D-3-phosphoglycerate into glucosyl-3-phosphoglycerate, which is then converted to 2-(alpha-D-glucopyranosyl)-D-glycerate by glucosyl-3-phosphoglycerate phosphatase (GpgP) Persephonella marina 2-O-(alpha-D-glucopyranosyl)-D-glycerate + ADP
-
 ?
2.4.1.268 GDP-alpha-D-glucose + D-glycerate
-
 Persephonella marina 2-O-(alpha-D-glucopyranosyl)-D-glycerate + GDP
-
 ?
2.4.1.268 additional information activity is not observed with: ADP-glucose, UDP-galactose, GDP-fucose, GDP-mannose or ADP-ribose. D-Glycerate is the only glucosylacceptor. No activity with mannose-6-phosphate, glucose-1-phosphate, glucose-6-phosphate, trehalose-6-phosphate, and fructose-6-phosphate or with the three-carbon compounds D-3-phosphoglycerate, D-2-phosphoglycerate, D-glycerate, L-glycerate, glycerol, and glycerol-3-phosphate Persephonella marina ?
-
 ?
2.4.1.268 UDP-glucose + D-glycerate
-
 Persephonella marina 2-O-(alpha-D-glucopyranosyl)-D-glycerate + UDP
-
 ?

Subunits

EC Number Subunits Comment Organism
2.4.1.268 tetramer 4 * 48000, SDS-PAGE Persephonella marina
2.4.1.268 tetramer 4 * 48400, calculated from sequence Persephonella marina

Synonyms

EC Number Synonyms Comment Organism
2.4.1.266 glucosyl-3-phosphoglycerate synthase
-
 Persephonella marina
2.4.1.266 GpgS
-
 Persephonella marina
2.4.1.268 Ggs
-
 Persephonella marina

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.4.1.266 70
-
 assay at Persephonella marina
2.4.1.268 80 85
-
 Persephonella marina

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
2.4.1.268 70 95 70°C: about 65% of maximal activity, 95°C: about 85% of maximal activity Persephonella marina

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
2.4.1.268 70
-
 1 week, no loss of activity Persephonella marina
2.4.1.268 85
-
 half-life: 74 h Persephonella marina
2.4.1.268 100
-
 half-life: 8 min Persephonella marina

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.4.1.266 6.5
-
 assay at Persephonella marina
2.4.1.268 6.5
-
-
 Persephonella marina

pH Range

EC Number pH Minimum pH Maximum Comment Organism
2.4.1.268 4.5 9 pH 4.5: about 50% of maximal activity, pH 9.0: about 35% of maximal activity Persephonella marina