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Literature summary extracted from
- Properties of R-citramalyl-coenzyme A lyase and its role in the autotrophic 3-hydroxypropionate cycle of Chloroflexus aurantiacus (2007), J. Bacteriol., 189, 2906-2914.
Activating Compound
| EC Number | Activating Compound | Comment | Organism | Structure |
|---|---|---|---|---|
| 4.1.3.46 | dithioerythritol | 4 mM, enhances the enzyme activity about threefold. The addition of both 4 mM dithioerythritol and 4 mM Mn2+ enhances the enzyme activity approximately 10fold compared to the results of an assay lacking dithioerythritol and Mn2+ | Chloroflexus aurantiacus |  |
| 4.1.3.46 | additional information | up-regulated severalfold during autotrophic growth | Chloroflexus aurantiacus |
Cloned(Commentary)
| EC Number | Cloned (Comment) | Organism |
|---|---|---|
| 4.1.3.46 | expressed in Escherichia coli | Chloroflexus aurantiacus |
| 4.1.3.46 | expression in Escherichia coli | Chloroflexus aurantiacus |
Inhibitors
| EC Number | Inhibitors | Comment | Organism | Structure |
|---|---|---|---|---|
| 4.1.3.46 | EDTA | activity is completely restored by the addition of excess Mn2+ | Chloroflexus aurantiacus | |
| 4.1.3.46 | iodoacetamide | in the absence of dithiothreitol, the addition of iodoacetamide (4 mM) results in complete loss of activity | Chloroflexus aurantiacus | |
| 4.1.3.46 | Zn2+ | - |
Chloroflexus aurantiacus | |
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 4.1.3.46 | 0.07 | - |
(3R)-citramalyl-CoA | pH 7.0, 55°C | Chloroflexus aurantiacus | |
Metals/Ions
| EC Number | Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|---|
| 4.1.3.46 | Co2+ | enzyme activity is stimulated by divalent cations in the order Mn2+, Co2+ > Ni2+ > Mg2+ | Chloroflexus aurantiacus | |
| 4.1.3.46 | copper | the recombinant enzyme contains 0.5 mol of zinc per mol of native enzyme. Additionally, 0.2 mol copper per mol native protein and traces of iron are detected | Chloroflexus aurantiacus | |
| 4.1.3.46 | Mg2+ | dependent | Chloroflexus aurantiacus | |
| 4.1.3.46 | Mg2+ | enzyme activity is stimulated by divalent cations in the order Mn2+, Co2+ > Ni2+ > Mg2+ | Chloroflexus aurantiacus | |
| 4.1.3.46 | Mn2+ | enzyme activity is stimulated by divalent cations in the order Mn2+, Co2+ > Ni2+ > Mg2+. The addition of both 4 mM dithioerythritol and 4 mM Mn2+ enhances the enzyme activity approximately 10fold compared to the results of an assay lacking dithioerythritol and Mn2+ | Chloroflexus aurantiacus | |
| 4.1.3.46 | additional information | no stimulation is obtained after the addition of Ca2+ | Chloroflexus aurantiacus | |
| 4.1.3.46 | Ni2+ | enzyme activity is stimulated by divalent cations in the order Mn2+, Co2+ > Ni2+ > Mg2+ | Chloroflexus aurantiacus | |
| 4.1.3.46 | Zinc | the recombinant enzyme contains 0.5 mol of zinc per mol of native enzyme. Additionally, 0.2 mol copper per mol native protein and traces of iron are detected | Chloroflexus aurantiacus | |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 4.1.3.46 | 34000 | - |
2 * 34000, SDS-PAGE | Chloroflexus aurantiacus |
| 4.1.3.46 | 65000 | - |
gel filtration | Chloroflexus aurantiacus |
| 4.1.3.46 | 68000 | - |
SDS-PAGE | Chloroflexus aurantiacus |
Natural Substrates/ Products (Substrates)
| EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 4.1.3.46 | (3R)-citramalyl-CoA | Chloroflexus aurantiacus | the enzyme is involved in the autotrophic 3-hydroxypropionate cycle, that results in the fixation of two molecules of bicarbonate into one molecule of glyoxylate | acetyl-CoA + pyruvate | - |
? | |
| 4.1.3.46 | (3R)-citramalyl-CoA | Chloroflexus aurantiacus DSM 636 | the enzyme is involved in the autotrophic 3-hydroxypropionate cycle, that results in the fixation of two molecules of bicarbonate into one molecule of glyoxylate | acetyl-CoA + pyruvate | - |
? | |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 4.1.3.46 | Chloroflexus aurantiacus | - |
- |
- |
| 4.1.3.46 | Chloroflexus aurantiacus DSM 636 | - |
- |
- |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 4.1.3.46 | - |
Chloroflexus aurantiacus |
Specific Activity [micromol/min/mg]
| EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|---|
| 4.1.3.46 | 0.02 | - |
recombinant enzyme | Chloroflexus aurantiacus |
| 4.1.3.46 | 1.52 | - |
pH 7.0, 55°C, purified enzyme | Chloroflexus aurantiacus |
Storage Stability
| EC Number | Storage Stability | Organism |
|---|---|---|
| 4.1.3.46 | 4°C, pH 7.0, no significant loss of activity | Chloroflexus aurantiacus |
| 4.1.3.46 | frozen for months in the presence of 10% glycerol (v/v) without significant loss of activity | Chloroflexus aurantiacus |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 4.1.3.46 | (3R)-citramalyl-CoA | - |
Chloroflexus aurantiacus | acetyl-CoA + pyruvate | - |
? | |
| 4.1.3.46 | (3R)-citramalyl-CoA | the enzyme is involved in the autotrophic 3-hydroxypropionate cycle, that results in the fixation of two molecules of bicarbonate into one molecule of glyoxylate | Chloroflexus aurantiacus | acetyl-CoA + pyruvate | - |
? | |
| 4.1.3.46 | (3R)-citramalyl-CoA | no activity with (3S)-citramalyl-CoA, , D-malyl-CoA, or L-malyl-CoA. cf. EC 4.1.3.25, (S)-citramalyl-CoA lyase | Chloroflexus aurantiacus | acetyl-CoA + pyruvate | - |
? | |
| 4.1.3.46 | (3R)-citramalyl-CoA | - |
Chloroflexus aurantiacus DSM 636 | acetyl-CoA + pyruvate | - |
? | |
| 4.1.3.46 | (3R)-citramalyl-CoA | the enzyme is involved in the autotrophic 3-hydroxypropionate cycle, that results in the fixation of two molecules of bicarbonate into one molecule of glyoxylate | Chloroflexus aurantiacus DSM 636 | acetyl-CoA + pyruvate | - |
? | |
| 4.1.3.46 | (3R)-citramalyl-CoA | no activity with (3S)-citramalyl-CoA, , D-malyl-CoA, or L-malyl-CoA. cf. EC 4.1.3.25, (S)-citramalyl-CoA lyase | Chloroflexus aurantiacus DSM 636 | acetyl-CoA + pyruvate | - |
? | |
Subunits
| EC Number | Subunits | Comment | Organism |
|---|---|---|---|
| 4.1.3.46 | homodimer | 2 * 34000, SDS-PAGE | Chloroflexus aurantiacus |
Synonyms
| EC Number | Synonyms | Comment | Organism |
|---|---|---|---|
| 4.1.3.45 | S-citramalyl-CoA lyase | - |
Chloroflexus aurantiacus |
| 4.1.3.45 | S-citramalyl-CoA lyase | - |
- |
| 4.1.3.45 | S-citramalyl-coenzyme A lyase | - |
Chloroflexus aurantiacus |
| 4.1.3.45 | S-citramalyl-coenzyme A lyase | - |
- |
| 4.1.3.46 | CCL | - |
Chloroflexus aurantiacus |
Temperature Optimum [°C]
| EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 4.1.3.46 | 55 | - |
assay at | Chloroflexus aurantiacus |
Turnover Number [1/s]
| EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 4.1.3.46 | 1.7 | - |
(3R)-citramalyl-CoA | pH 7.0, 55°C | Chloroflexus aurantiacus | |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 4.1.3.46 | 7 | - |
at 55°C | Chloroflexus aurantiacus |
pH Range
| EC Number | pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|---|
| 4.1.3.46 | 6.3 | 7.6 | half-maximal activities at pHs of 6.3 and 7.6 | Chloroflexus aurantiacus |
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