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Literature summary extracted from

  • Espie, G.S.; Jalali, F.; Tong, T.; Zacal, N.J.; So, A.K.
    Involvement of the cynABDS operon and the CO2-concentrating mechanism in the light-dependent transport and metabolism of cyanate by cyanobacteria (2007), J. Bacteriol., 189, 1013-1024.
    View publication on PubMedView publication on EuropePMC

Application

EC Number Application Comment Organism
4.2.1.104 molecular biology analyses of conditions to metabolize exogenously supplied cyanate Synechocystis sp.
4.2.1.104 molecular biology analyses of conditions to metabolize exogenously supplied cyanate, depending on proteins of the Cyn-ABDS operon, light, and on activity of the CO2-concentrating mechanism (CCM), low internal pools of HCO3- and CO2 result in an insufficient supply of bicarbonate Synechococcus elongatus
4.2.1.104 molecular biology analyses of conditions to metabolize exogenously supplied cyanate, depending on proteins of the CynABDS operon, light, and on activity of the CO2-concentrating mechanism (CCM), inactivation of the cynS gene leads to inability of decomposition of external cyanate Synechococcus elongatus
4.2.1.104 molecular biology analyses of conditions to metabolize exogenously supplied cyanate, depending on proteins of the CynABDS operon, light, and on activity of the CO2-concentrating mechanism (CCM), mutagensis of a periplasmatic binding protein of a multicomponent ABC-transporter (CynA), leads to inability of decomposition of external cyanate due to impaired cyanate uptake, cyanase function is not affected Synechococcus elongatus
4.2.1.104 molecular biology analyses of conditions to metabolize exogenously supplied cyanate, depending on proteins of the operon CynABDS, light and internal pools of HCO3- and CO2 Synechococcus sp.

Cloned(Commentary)

EC Number Cloned (Comment) Organism
4.2.1.104 expressed in Escherichia coli Synechococcus elongatus
4.2.1.104 expressed in Escherichia coli Synechococcus sp.
4.2.1.104 expressed in Escherichia coli, cyanase gene cynS not arranged in a three-gene cluster Synechocystis sp.

Protein Variants

EC Number Protein Variants Comment Organism
4.2.1.104 additional information targeted mutants of Synechococcus elongatus strain PCC7942 disrupted in the coding sequences of the gene encoding the cytosoliyc cyanase (CynS) to study metabolism of exogenously supplied cyanate Synechococcus elongatus
4.2.1.104 additional information targeted mutants of Synechococcus elongatus strain PCC7942 impaired in the CO2-concentrating mechanism CCM to study metabolism of exogenously supplied cyanate in cyanobacteria, inactivation of CCM impairs metabolism of external cyanate by insufficient internal pools of HCO3- and CO2 resulting in low levels of the co-substrate bicarbonate that is required for cyanase activity Synechococcus elongatus
4.2.1.104 additional information targeted mutants of Synechococcus elongatus strain PCC7942, disruption of the cynA gene, encoding a binding protein of a multicomponent ABC-transporter, inactivation of CynA does not affect cyanase function but inhibits decomposition of external cyanate Synechococcus elongatus

Inhibitors

EC Number Inhibitors Comment Organism Structure
4.2.1.104 oxalate the membrane-impermeant inhibitor of cyanobacterial cyanase has minor effects on acid-labile or acid-stable fractions indicating the absence of cyanase from the periplasm Synechococcus elongatus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
4.2.1.104 cytosol
-
 Synechococcus sp. 5829
-
4.2.1.104 cytosol
-
 Synechococcus elongatus 5829
-
4.2.1.104 cytosol the cytosolic cyanase is encoded by the cynS gene, part of the operon CynABDS Synechococcus elongatus 5829
-

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
4.2.1.104 cyanate + HCO3- + 2 H+ Synechococcus sp.
-
 NH3 + 2 CO2
-
 ?
4.2.1.104 cyanate + HCO3- + 2 H+ Synechococcus elongatus
-
 NH3 + 2 CO2
-
 ?
4.2.1.104 cyanate + HCO3- + 2 H+ Synechococcus elongatus PCC 7942
-
 NH3 + 2 CO2
-
 ?
4.2.1.104 cyanate + HCO3- + 2 H+ Synechococcus sp. UTEX625
-
 NH3 + 2 CO2
-
 ?
4.2.1.104 NCO- + HCO3- + H+ Synechococcus elongatus utilization of exogenous cyanate as a niche source of C and N in cyanobacteria NH3 + CO2
-
 ?
4.2.1.104 NCO- + HCO3- + H+ Synechococcus sp. utilization of exogenous cyanate as a niche source of C and N in cyanobacteria NH3 + CO2
-
 ?
4.2.1.104 NCO- + HCO3- + H+ Synechocystis sp. utilization of exogenous cyanate as a niche source of C and N in cyanobacteria NH3 + CO2
-
 ?
4.2.1.104 NCO- + HCO3- + H+ Synechococcus elongatus utilization of exogenous cyanate as a niche source of C and N in cyanobacteria NH4+ + CO2
-
 ?
4.2.1.104 NCO- + HCO3- + H+ Synechococcus elongatus PCC 7942 utilization of exogenous cyanate as a niche source of C and N in cyanobacteria NH3 + CO2
-
 ?
4.2.1.104 NCO- + HCO3- + H+ Synechococcus elongatus PCC 7942 utilization of exogenous cyanate as a niche source of C and N in cyanobacteria NH4+ + CO2
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
4.2.1.104 Synechococcus elongatus
-
 strain PCC7942
-
4.2.1.104 Synechococcus elongatus Q59948 strain PCC7942, CynA mutant, the cynA gene encodes a binding protein of an ABC transporter, part of the CynABDS operon
-
4.2.1.104 Synechococcus elongatus Q59948 strain PCC7942, CynS mutant, disruption of the cynS gene encoding cyanase
-
4.2.1.104 Synechococcus elongatus Q59948 strain PCC7942, mutant DELTA-chpX-DELTA-chpY, disturbed activity of the CO2-concentrating mechanism (CCM)
-
4.2.1.104 Synechococcus elongatus PCC 7942
-
 strain PCC7942
-
4.2.1.104 Synechococcus elongatus PCC 7942 Q59948 strain PCC7942, CynA mutant, the cynA gene encodes a binding protein of an ABC transporter, part of the CynABDS operon
-
4.2.1.104 Synechococcus elongatus PCC 7942 Q59948 strain PCC7942, CynS mutant, disruption of the cynS gene encoding cyanase
-
4.2.1.104 Synechococcus elongatus PCC 7942 Q59948 strain PCC7942, mutant DELTA-chpX-DELTA-chpY, disturbed activity of the CO2-concentrating mechanism (CCM)
-
4.2.1.104 Synechococcus sp.
-
-
-
4.2.1.104 Synechococcus sp. Q5N0J1 strain UTEX625, also known as Synechococcus sp. strain PCC6301
-
4.2.1.104 Synechococcus sp. UTEX625
-
-
-
4.2.1.104 Synechocystis sp. Q55367 strain PCC6803
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
4.2.1.104 additional information
-
 cyanase activity shown in whole-cell extracts of Synechocystis, high level of cyanase activity when grown with nitrate as N source but unable to utilize exogenous cyanate to support O2 evolution in the light Synechocystis sp.
4.2.1.104 additional information
-
 cyanase activity shown in whole-cell extracts of the photoautotrophic Synechococcus sp. strain UTEX 625, ability to utilize cyanate-derived CO2 to support photosynthesis affected Synechococcus sp.

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.2.1.104 cyanate + HCO3- + 2 H+
-
 Synechococcus sp. NH3 + 2 CO2
-
 ?
4.2.1.104 cyanate + HCO3- + 2 H+
-
 Synechococcus elongatus NH3 + 2 CO2
-
 ?
4.2.1.104 cyanate + HCO3- + 2 H+
-
 Synechococcus elongatus PCC 7942 NH3 + 2 CO2
-
 ?
4.2.1.104 cyanate + HCO3- + 2 H+
-
 Synechococcus sp. UTEX625 NH3 + 2 CO2
-
 ?
4.2.1.104 NCO- + HCO3- + H+ utilization of exogenous cyanate as a niche source of C and N in cyanobacteria Synechococcus elongatus NH3 + CO2
-
 ?
4.2.1.104 NCO- + HCO3- + H+ utilization of exogenous cyanate as a niche source of C and N in cyanobacteria Synechococcus sp. NH3 + CO2
-
 ?
4.2.1.104 NCO- + HCO3- + H+ utilization of exogenous cyanate as a niche source of C and N in cyanobacteria Synechocystis sp. NH3 + CO2
-
 ?
4.2.1.104 NCO- + HCO3- + H+ studies on decomposition of externally supplied cyanate in cyanobacteria Synechocystis sp. NH3 + CO2
-
 ?
4.2.1.104 NCO- + HCO3- + H+ studies on decomposition of externally supplied cyanate in photoautotroph cyanobacteria Synechococcus sp. NH3 + CO2
-
 ?
4.2.1.104 NCO- + HCO3- + H+ studies on decomposition of externally supplied cyanate, depending on the CynABDS operon, light and internal pools of HCO3- and CO2 Synechococcus elongatus NH3 + CO2
-
 ?
4.2.1.104 NCO- + HCO3- + H+ utilization of exogenous cyanate as a niche source of C and N in cyanobacteria Synechococcus elongatus PCC 7942 NH3 + CO2
-
 ?
4.2.1.104 NCO- + HCO3- + H+ studies on decomposition of externally supplied cyanate, depending on the CynABDS operon, light and internal pools of HCO3- and CO2 Synechococcus elongatus PCC 7942 NH3 + CO2
-
 ?
4.2.1.104 NCO- + HCO3- + H+ utilization of exogenous cyanate as a niche source of C and N in cyanobacteria Synechococcus elongatus NH4+ + CO2
-
 ?
4.2.1.104 NCO- + HCO3- + H+ utilization of exogenous cyanate as a niche source of C and N in cyanobacteria Synechococcus elongatus PCC 7942 NH4+ + CO2
-
 ?
4.2.1.104 NCO- + HCO3- + H+ studies on decomposition of externally supplied cyanate, depending on the CynABDS operon, light and internal pools of HCO3- and CO2 Synechococcus elongatus NH4+ + 2 CO2
-
 ?
4.2.1.104 NCO- + HCO3- + H+ studies on decomposition of externally supplied cyanate, depending on the CynABDS operon, light and internal pools of HCO3- and CO2 Synechococcus elongatus PCC 7942 NH4+ + 2 CO2
-
 ?

Synonyms

EC Number Synonyms Comment Organism
4.2.1.104 cyanase
-
 Synechococcus elongatus
4.2.1.104 cyanase
-
 Synechococcus sp.
4.2.1.104 cyanase
-
 Synechocystis sp.