EC Number | Cloned (Comment) | Organism |
---|---|---|
3.2.1.20 | gene aglA, expression analysis and mapping of transcription start sites, the gene is clustered in the genome and shows colinear orientation and a small intergenic space with gene manA encoding alpha-mannosidase, the genes are independently transcribed, both producing leaderless mRNA, phylogenetic tree, overexpression in Escherichia coli strain BL21, subcloning in Escherichia coli strain XL-1 Blue | Picrophilus torridus |
3.2.1.24 | overexpressed in Escherichia coli | Picrophilus torridus |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
3.2.1.24 | Ag+ | strong inhibitor at concentrations of 1 mM or 10 mM | Picrophilus torridus | |
3.2.1.24 | Cu2+ | strong inhibitor at concentrations of 1 mM or 10 mM | Picrophilus torridus | |
3.2.1.24 | EDTA | supplied in equimolar amounts or in excess, neutralizes also effect of resistance of ManA to heat inactivation | Picrophilus torridus | |
3.2.1.24 | Fe3+ | strong inhibitor at concentrations of 1 mM or 10 mM | Picrophilus torridus | |
3.2.1.24 | additional information | 1-deoxymannojirimycin has no effect on ManA at concentrations up to 500 microM | Picrophilus torridus | |
3.2.1.24 | Ni2+ | strong inhibitor at concentrations of 1 mM or 10 mM | Picrophilus torridus | |
3.2.1.24 | swainsonine | shows competitive inhibition when p-nitrophenyl-Man is used as a substrate | Picrophilus torridus |
EC Number | Metals/Ions | Comment | Organism | Structure |
---|---|---|---|---|
3.2.1.24 | Cd2+ | potent activator at concentration of 1 mM, 12.5fold activation compared to the apoenzyme | Picrophilus torridus | |
3.2.1.24 | Co2+ | 8.3fold activation at a concentration of 10 mM, resistance of ManA to heat inactivation is increased by presence of Co2+ | Picrophilus torridus | |
3.2.1.24 | Fe2+ | 4.5fold activation at a concentration of 10 mM | Picrophilus torridus | |
3.2.1.24 | Mn2+ | 3.8fold activation at a concentration of 10 mM | Picrophilus torridus | |
3.2.1.24 | additional information | Zn2+, Ca2+, Mg2+, Ba2+, K+, and Li+ had no significant effect on ManA activity at a concentration of 1 mM or 10 mM | Picrophilus torridus |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
3.2.1.20 | 72000 | - |
6 * 72000, recombinant AglA, SDS-PAGE | Picrophilus torridus |
3.2.1.20 | 440000 | - |
recombinant AglA, gel filtration | Picrophilus torridus |
3.2.1.24 | 111500 | - |
2 * 111500, gel filtration | Picrophilus torridus |
3.2.1.24 | 120000 | - |
small fraction of ManA activity, reflecting single subunits | Picrophilus torridus |
3.2.1.24 | 220000 | - |
gel filtration | Picrophilus torridus |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
3.2.1.20 | maltose + H2O | Picrophilus torridus | probable physiological role of the enzyme in the utilization of exogenous glycoproteins and/or in the turnover of the organisms own glycoproteins, overview | alpha-D-glucose + D-glucose | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
3.2.1.20 | Picrophilus torridus | Q6L2X4 | strain DSM 9790, gene aglA | - |
3.2.1.24 | Picrophilus torridus | Q6L2X5 | extremophilic organism belonging to the euryarchaeal phylum | - |
EC Number | Purification (Comment) | Organism |
---|---|---|
3.2.1.20 | recombinant AglA 39.3fold from Escherichia coli strain BL21 by heat treatment for 15 min at 67°C, hydrophobic interaction and anion exchange chromatography, and gel filtration in presence of 150 mM NaCl | Picrophilus torridus |
3.2.1.24 | SDS-PAGE, anion-exchange, size exclusion chromatography | Picrophilus torridus |
EC Number | Source Tissue | Comment | Organism | Textmining |
---|---|---|---|---|
3.2.1.20 | additional information | 60°C is the optimal, 67°C the maximal growth temperature | Picrophilus torridus | - |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
3.2.1.24 | 6.6 | - |
ManA is equally active with mannobiose substrates, released mannose is quantified, resulting in a specific activity of approximately 6.6 U/mg | Picrophilus torridus |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
3.2.1.20 | 4-nitrophenyl alpha-D-glucopyranoside + H2O | low activity | Picrophilus torridus | 4-nitrophenol + alpha-D-glucose | - |
? | |
3.2.1.20 | dextrin 10 + H2O | 27% of the activity with maltose | Picrophilus torridus | alpha-D-glucose | - |
? | |
3.2.1.20 | maltohexaose + H2O | - |
Picrophilus torridus | maltopentaose + D-glucose | - |
? | |
3.2.1.20 | maltopentaose + H2O | - |
Picrophilus torridus | alpha-D-glucose + ? | - |
? | |
3.2.1.20 | maltose + H2O | probable physiological role of the enzyme in the utilization of exogenous glycoproteins and/or in the turnover of the organisms own glycoproteins, overview | Picrophilus torridus | alpha-D-glucose + D-glucose | - |
? | |
3.2.1.20 | maltose + H2O | preferred substrate, the enzyme shows strict glucose substrate specificity hydrolyzing maltose, as well as longer alpha-1,4-linked maltooligosaccharides | Picrophilus torridus | alpha-D-glucose + D-glucose | - |
? | |
3.2.1.20 | maltotetraose + H2O | 54% of the activity with maltose | Picrophilus torridus | alpha-D-glucose | - |
? | |
3.2.1.20 | maltotriose + H2O | - |
Picrophilus torridus | maltose + alpha-D-glucose | - |
? | |
3.2.1.24 | 1,2-alpha-D-mannobiose + H2O | - |
Picrophilus torridus | alpha-D-mannose | - |
? | |
3.2.1.24 | 1,3-alpha-D-mannobiose + H2O | - |
Picrophilus torridus | alpha-D-mannose | - |
? | |
3.2.1.24 | 1,6-alpha-D-mannobiose + H2O | - |
Picrophilus torridus | alpha-D-mannose | - |
? | |
3.2.1.24 | alpha3,alpha6-D-mannopentaose + H2O | - |
Picrophilus torridus | alpha-D-mannose | - |
? | |
3.2.1.24 | methyl-O-1,4-alpha-D-mannobiose + H2O | - |
Picrophilus torridus | alpha-D-mannose | - |
? | |
3.2.1.24 | p-nitrophenyl-alpha-D-mannopyranoside + H2O | - |
Picrophilus torridus | 4-nitrophenol + alpha-D-mannose | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
3.2.1.20 | hexamer | 6 * 72000, recombinant AglA, SDS-PAGE | Picrophilus torridus |
3.2.1.24 | dimer | 2 * 111500, gel filtration | Picrophilus torridus |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
3.2.1.20 | AglA | - |
Picrophilus torridus |
3.2.1.24 | alpha-mannosidase | - |
Picrophilus torridus |
3.2.1.24 | ManA | - |
Picrophilus torridus |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
3.2.1.20 | 87 | - |
recombinant AglA | Picrophilus torridus |
3.2.1.24 | 70 | - |
maximum activity of ManA | Picrophilus torridus |
EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|---|
3.2.1.20 | 100 | - |
15 min, inactivation | Picrophilus torridus |
3.2.1.24 | 80 | - |
resistance of ManA to heat inactivation (at 80°C) | Picrophilus torridus |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
3.2.1.20 | 5 | - |
- |
Picrophilus torridus |
3.2.1.24 | 5.2 | - |
maximum activity of ManA | Picrophilus torridus |
EC Number | Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|---|
3.2.1.24 | 0.00122 | - |
swainsonine | - |
Picrophilus torridus |