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Literature summary extracted from

  • Costa, J.; Empadinhas, N.; Goncalves, L.; Lamosa, P.; Santos, H.; da Costa, M.S.
    Characterization of the biosynthetic pathway of glucosylglycerate in the archaeon Methanococcoides burtonii (2006), J. Bacteriol., 188, 1022-1030.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.4.1.266 expressed in Escherichia coli Methanococcoides burtonii
3.1.3.85 expressed in Escherichia coli Methanococcoides burtonii

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.4.1.266 0.54
-
 3-phospho-D-glycerate pH 7.6, 30°C Methanococcoides burtonii
2.4.1.266 1.6
-
 3-phospho-D-glycerate pH 7.6, 50°C Methanococcoides burtonii
2.4.1.266 1.75
-
 GDP-glucose pH 7.6, 30°C Methanococcoides burtonii
2.4.1.266 3.7
-
 GDP-glucose pH 7.6, 50°C Methanococcoides burtonii
3.1.3.85 0.08
-
 2-O-(alpha-D-glucopyranosyl)-3-phospho-D-glycerate pH 7.6, 30°C Methanococcoides burtonii
3.1.3.85 0.1
-
 2-O-(alpha-D-glucopyranosyl)-3-phospho-D-glycerate pH 7.6, 30°C Thermus thermophilus
3.1.3.85 0.11
-
 2-O-(alpha-D-mannopyranosyl)-3-phospho-D-glycerate pH 7.6, 30°C Thermus thermophilus
3.1.3.85 0.27
-
 2-O-(alpha-D-glucopyranosyl)-3-phospho-D-glycerate pH 7.6, 50°C Methanococcoides burtonii
3.1.3.85 0.28
-
 2-O-(alpha-D-mannopyranosyl)-3-phospho-D-glycerate pH 7.6, 50°C Methanococcoides burtonii
3.1.3.85 0.36
-
 2-O-(alpha-D-glucopyranosyl)-3-phospho-D-glycerate pH 7.6, 50°C Thermus thermophilus
3.1.3.85 0.37
-
 2-O-(alpha-D-mannopyranosyl)-3-phospho-D-glycerate pH 7.6, 50°C Thermus thermophilus
3.1.3.85 0.79
-
 2-O-(alpha-D-glucopyranosyl)-3-phospho-D-glycerate pH 7.6, 70°C Thermus thermophilus
3.1.3.85 0.83
-
 2-O-(alpha-D-mannopyranosyl)-3-phospho-D-glycerate pH 7.6, 70°C Thermus thermophilus
3.1.3.85 0.9
-
 2-O-(alpha-D-mannopyranosyl)-3-phospho-D-glycerate pH 7.6, 90°C Thermus thermophilus
3.1.3.85 1.1
-
 2-O-(alpha-D-glucopyranosyl)-3-phospho-D-glycerate pH 7.6, 90°C Thermus thermophilus

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.4.1.266 Co2+ strictly dependent on divalent cations in the following order of efficiency: Mn2+, Co2+, Mg2+. Maximal activity at 1 mM Methanococcoides burtonii
2.4.1.266 Mg2+ strictly dependent on divalent cations in the following order of efficiency: Mn2+, Co2+, Mg2+ Methanococcoides burtonii
2.4.1.266 Mn2+ strictly dependent on divalent cations in the following order of efficiency: Mn2+, Co2+, Mg2+. Maximal activity at 1 mM Methanococcoides burtonii
2.4.1.266 additional information K+, Ca2+, Sr2+, Cu2+, Ba2+, and Zn2+, do not stimulate GpgS activity at any concentration Methanococcoides burtonii
3.1.3.85 Co2+ at 50°C, divalent cations are absolutely required for GpgP activity with 2-O-(alpha-D-glucosyl)-3-phospho-D-glycerate as the substrate. However, when 2-O-(alpha-D-mannosyl)-3-phospho-D-glycerate is the substrate, 3% activity is detected without cations, and the preferred metal ion with both substrates is Co2+ (10 mM). At 30°C, GpgP retains 37% of its enzyme activity towards 2-O-(alpha-D-glucosyl)-3-phospho-D-glycerate and 12% activity towards 2-O-(alpha-D-mannosyl)-3-phospho-D-glycerate in a reaction mixture without cations compared to the activity with 10 mM K+ and Co2+ Methanococcoides burtonii
3.1.3.85 K+ at 30°C, GpgP retains 37% of its enzyme activity towards 2-O-(alpha-D-glucosyl)-3-phospho-D-glycerate and 12% activity towards 2-O-(alpha-D-mannosyl)-3-phospho-D-glycerate in a reaction mixture without cations compared to the activity with 10 mM K+ and Co2+. Potassium chloride, in the concentration range of 10 to 100 mM, stimulates enzyme activity about 40% at 50°C, but only when 2-O-(alpha-D-mannosyl)-3-phospho-D-glycerate is the substrate. However, at 30°C, K+ has a stimulatory effect towards 2-O-(alpha-D-glucosyl)-3-phospho-D-glycerate, but not with 2-O-(alpha-D-mannosyl)-3-phospho-D-glycerate as the substrate Methanococcoides burtonii
3.1.3.85 Mg2+ at 50°C, divalent cations are absolutely required for GpgP activity with 2-O-(alpha-D-glucosyl)-3-phospho-D-glycerate as the substrate. Mg2+ and, to a lesser extent, Ni2+ can replace Co2+ Methanococcoides burtonii
3.1.3.85 Mn2+ enzyme activity is not detected with Mn2+ when 2-O-(alpha-D-glucosyl)-3-phospho-D-glycerate is used, while Mn2+ had a strong stimulatory effect (85% of the maximal activity with Co2+) with 2-O-(alpha-D-mannosyl)-3-phospho-D-glycerate Methanococcoides burtonii
3.1.3.85 additional information Ca2+, Sr2+, Ba2+, Cu2+, and Zn2+, have no effect on GpgP activity at any concentration Methanococcoides burtonii
3.1.3.85 Ni2+ at 50°C, divalent cations are absolutely required for GpgP activity with 2-O-(alpha-D-glucosyl)-3-phospho-D-glycerate as the substrate. Mg2+ and, to a lesser extent, Ni2+ can replace Co2+ Methanococcoides burtonii

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.4.1.266 43000
-
 x * 43000, calculated from sequence Methanococcoides burtonii

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.4.1.266 GDP-glucose + 3-phospho-D-glycerate Methanococcoides burtonii synthesis of the solute glucosylglycerate proceeds via a two-step pathway involving glucosyl-3-phosphoglycerate synthase (GpgS) and glucosyl-3-phosphoglycerate phosphatase (GpgP). An mpgS gene coding for mannosyl-3-phosphoglycerate synthase (MpgS) is absent GDP + 2-O-(alpha-D-glucopyranosyl)-3-phospho-D-glycerate
-
 ?
3.1.3.85 2-O-(alpha-D-glucopyranosyl)-3-phospho-D-glycerate + H2O Methanococcoides burtonii synthesis of the solute glucosylglycerate proceeds via a two-step pathway involving glucosyl-3-phosphoglycerate synthase (GpgS) and glucosyl-3-phosphoglycerate phosphatase (GpgP) 2-O-(alpha-D-glucopyranosyl)-D-glycerate + phosphate
-
 ?
3.1.3.85 2-O-(alpha-D-glucopyranosyl)-3-phospho-D-glycerate + H2O Thermus thermophilus synthesis of the solute glucosylglycerate proceeds via a two-step pathway involving glucosyl-3-phosphoglycerate synthase (GpgS) and glucosyl-3-phosphoglycerate phosphatase (GpgP) 2-O-(alpha-D-glucopyranosyl)-D-glycerate + phosphate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.4.1.266 Methanococcoides burtonii Q12XX4
-
-
3.1.3.85 Methanococcoides burtonii
-
-
-
3.1.3.85 Thermus thermophilus Q84B23
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.4.1.266 recombinant enzyme Methanococcoides burtonii
3.1.3.85 recombinant enzyme Methanococcoides burtonii

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.4.1.266 0.0116
-
 pH 7.6, 30°C Methanococcoides burtonii

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.4.1.266 GDP-glucose + 3-phospho-D-glycerate
-
 Methanococcoides burtonii GDP + 2-O-(alpha-D-glucopyranosyl)-3-phospho-D-glycerate
-
 ?
2.4.1.266 GDP-glucose + 3-phospho-D-glycerate synthesis of the solute glucosylglycerate proceeds via a two-step pathway involving glucosyl-3-phosphoglycerate synthase (GpgS) and glucosyl-3-phosphoglycerate phosphatase (GpgP). An mpgS gene coding for mannosyl-3-phosphoglycerate synthase (MpgS) is absent Methanococcoides burtonii GDP + 2-O-(alpha-D-glucopyranosyl)-3-phospho-D-glycerate
-
 ?
2.4.1.266 additional information no activity with: ADP-ribose, UDP-acetylgalactosamine, UDP-glucuronic acid, UDP-galactose, GDP-fucose, GDP-mannose, UDP-mannose, ADP-mannose, UDP-glucose, TDP-glucose, and ADP-glucose as sugar donors and glycerol, D-2-phosphoglycerate, L-glycerol-3-phosphate, 2,3-diphospho-D-glycerate, and phosphoenolpyruvate Methanococcoides burtonii ?
-
 ?
3.1.3.85 2-O-(alpha-D-glucopyranosyl)-3-phospho-D-glycerate + H2O synthesis of the solute glucosylglycerate proceeds via a two-step pathway involving glucosyl-3-phosphoglycerate synthase (GpgS) and glucosyl-3-phosphoglycerate phosphatase (GpgP) Methanococcoides burtonii 2-O-(alpha-D-glucopyranosyl)-D-glycerate + phosphate
-
 ?
3.1.3.85 2-O-(alpha-D-glucopyranosyl)-3-phospho-D-glycerate + H2O synthesis of the solute glucosylglycerate proceeds via a two-step pathway involving glucosyl-3-phosphoglycerate synthase (GpgS) and glucosyl-3-phosphoglycerate phosphatase (GpgP) Thermus thermophilus 2-O-(alpha-D-glucopyranosyl)-D-glycerate + phosphate
-
 ?
3.1.3.85 2-O-(alpha-D-glucopyranosyl)-3-phospho-D-glycerate + H2O the Vmax/Km-value for 2-O-(alpha-D-glucopyranosyl)-3-phospho-D-glycerate is about 2fold higher than the value for 2-O-(alpha-D-mannopyranosyl)-3-phospho-D-glycerate at 30°C and at 50°C. The Vmax/Km-value for 2-O-(alpha-D-glucopyranosyl)-3-phospho-D-glycerate is similar to the value for 2-O-(alpha-D-mannopyranosyl)-3-phospho-D-glycerate at 70°C and at 90°C Thermus thermophilus 2-O-(alpha-D-glucopyranosyl)-D-glycerate + phosphate
-
 ?
3.1.3.85 2-O-(alpha-D-glucopyranosyl)-3-phospho-D-glycerate + H2O the Vmax/Km-value for 2-O-(alpha-D-glucosyl)-3-phospho-D-glycerate is about 2fold higher than the value for 2-O-(alpha-D-mannosyl)-3-phospho-D-glycerate Methanococcoides burtonii 2-O-(alpha-D-glucopyranosyl)-D-glycerate + phosphate
-
 ?
3.1.3.85 2-O-(alpha-D-mannopyranosyl)-3-phospho-D-glycerate + H2O the Vmax/Km-value for 2-O-(alpha-D-glucopyranosyl)-3-phospho-D-glycerate is about 2fold higher than the value for 2-O-(alpha-D-mannopyranosyl)-3-phospho-D-glycerate at 30°C and at 50°C. The Vmax/Km-value for 2-O-(alpha-D-glucopyranosyl)-3-phospho-D-glycerate is similar to the value for 2-O-(alpha-D-mannopyranosyl)-3-phospho-D-glycerate at 70°C and at 90°C Thermus thermophilus 2-O-(alpha-D-mannopyranosyl)-D-glycerate + phosphate
-
 ?
3.1.3.85 2-O-(alpha-D-mannopyranosyl)-3-phospho-D-glycerate + H2O the Vmax/Km-value for 2-O-(alpha-D-glucosyl)-3-phospho-D-glycerate is about 2fold higher than the value for 2-O-(alpha-D-mannopyranosyl)-3-phospho-D-glycerate Methanococcoides burtonii 2-O-(alpha-D-mannopyranosyl)-D-glycerate + phosphate
-
 ?

Subunits

EC Number Subunits Comment Organism
2.4.1.266 ? x * 43000, calculated from sequence Methanococcoides burtonii

Synonyms

EC Number Synonyms Comment Organism
2.4.1.266 GpgS
-
 Methanococcoides burtonii
3.1.3.85 GpgP
-
 Methanococcoides burtonii
3.1.3.85 GpgP
-
 Thermus thermophilus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.4.1.266 50
-
-
 Methanococcoides burtonii
3.1.3.85 50
-
 substrate: 2-O-(alpha-D-glucopyranosyl)-3-phospho-D-glycerate Methanococcoides burtonii
3.1.3.85 60
-
 substrate: 2-O-(alpha-D-mannopyranosyl)-3-phospho-D-glycerate Methanococcoides burtonii

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
2.4.1.266 30 70 30°C: about 50% of maximal activity, 70°C: about 50% of maximal activity Methanococcoides burtonii
3.1.3.85 30 70 30°C: about 55% of maximal activity, 70°C: about 50% of maximal activity, substrate: 2-O-(alpha-D-glucopyranosyl)-3-phospho-D-glycerate Methanococcoides burtonii
3.1.3.85 50 70 50°C: about 60% of maximal activity, 70°C: about 60% of maximal activity, substrate: 2-O-(alpha-D-mannopyranosyl)-3-phospho-D-glycerate Methanococcoides burtonii

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
2.4.1.266 4
-
 half-life: 51.3 h Methanococcoides burtonii
2.4.1.266 23
-
 half-life: 11.5 h Methanococcoides burtonii
2.4.1.266 50
-
 half-life: 13.8 h Methanococcoides burtonii
3.1.3.85 4
-
 half-life: 198 h Methanococcoides burtonii
3.1.3.85 23
-
 half-life: 165 h Methanococcoides burtonii
3.1.3.85 50
-
 half-life: 12 min Methanococcoides burtonii

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.4.1.266 7.5 8.5
-
 Methanococcoides burtonii
3.1.3.85 5.5
-
 substrate: 2-O-(alpha-D-glucopyranosyl)-3-phospho-D-glycerate Methanococcoides burtonii
3.1.3.85 6.5
-
 substrate: 2-O-(alpha-D-mannopyranosyl)-3-phospho-D-glycerate Methanococcoides burtonii

pH Range

EC Number pH Minimum pH Maximum Comment Organism
2.4.1.266 6.5 9.5 pH 6.5: about 55% of maximal activity, pH 9.5: about 70% of maximal activity Methanococcoides burtonii
3.1.3.85 4.5 9 pH 4.5: about 40% of maximal activity, pH 9.0: about 45% of maximal activity, substrate: 2-O-(alpha-D-glucopyranosyl)-3-phospho-D-glycerate Methanococcoides burtonii

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
3.1.3.85 additional information
-
 additional information the Vmax/Km-value for 2-O-(alpha-D-glucopyranisyl)-3-phospho-D-glycerate is about 2fold higher than the value for 2-O-(alpha-D-mannopyranosyl)-3-phospho-D-glycerate at 30°C and at 50°C. The Vmax/Km-value for 2-O-(alpha-D-glucopyranosyl)-3-phospho-D-glycerate is similat to the value for 2-O-(alpha-D-mannopyranosyl)-3-phospho-D-glycerate at 70°C and at 90°C Thermus thermophilus
3.1.3.85 additional information
-
 additional information the Vmax/Km-value for 2-O-(alpha-D-glucopyranosyl)-3-phospho-D-glycerate is about 2fold higher than the value for 2-O-(alpha-D-mannopyranosyl)-3-phospho-D-glycerate Methanococcoides burtonii