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Literature summary extracted from

  • Tanaka, T.; Fukui, T.; Atomi, H.; Imanaka, T.
    Characterization of an exo-beta-D-glucosaminidase involved in a novel chitinolytic pathway from the hyperthermophilic archaeon Thermococcus kodakaraensis KOD1 (2003), J. Bacteriol., 185, 5175-5181.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.2.1.165 expression in Escherichia coli BL21 Thermococcus kodakarensis

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.2.1.165 0.0778
-
 chitopentaose pH 6.0, 80°C Thermococcus kodakarensis
3.2.1.165 0.27
-
 chitotriose pH 6.0, 80°C Thermococcus kodakarensis
3.2.1.165 0.295
-
 chitotetraose pH 6.0, 80°C Thermococcus kodakarensis
3.2.1.165 0.365
-
 chitohexaose pH 6.0, 80°C Thermococcus kodakarensis
3.2.1.165 1.37
-
 chitobiose pH 6.0, 80°C Thermococcus kodakarensis

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.1.165 86000
-
 2 * 86000, SDS-PAGE Thermococcus kodakarensis
3.2.1.165 193000
-
 gel filtration Thermococcus kodakarensis

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.165 Thermococcus kodakarensis Q76HN4
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.165 recombinant enzyme Thermococcus kodakarensis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.165 4-methylumbelliferyl beta-D-glucoside + H2O
-
 Thermococcus kodakarensis 4-methylumbelliferol + D-glucose
-
 ?
3.2.1.165 chitobiose + H2O
-
 Thermococcus kodakarensis D-glucosamine
-
 ?
3.2.1.165 chitohexaose + H2O at the early stages of the reaction, chitohexaose is converted to D-glucosamine and chitopentaose Thermococcus kodakarensis D-glucosamine
-
 ?
3.2.1.165 chitopentaose + H2O at the early stages of the reaction, chitopentaose is converted to D-glucosamine and chitotetraose Thermococcus kodakarensis D-glucosamine
-
 ?
3.2.1.165 chitotetraose + H2O at the early stages of the reaction, chitotetraose is converted to D-glucosamine and chitotriose Thermococcus kodakarensis D-glucosamine
-
 ?
3.2.1.165 chitotriose + H2O
-
 Thermococcus kodakarensis D-glucosamine
-
 ?

Subunits

EC Number Subunits Comment Organism
3.2.1.165 dimer 2 * 86000, SDS-PAGE Thermococcus kodakarensis

Synonyms

EC Number Synonyms Comment Organism
3.2.1.165 exo-beta-D-glucosaminidase
-
 Thermococcus kodakarensis

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.165 80
-
-
 Thermococcus kodakarensis

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
3.2.1.165 37 100 37°C: about 20% of maximal activity, 100°C: about 20% of maximal activity Thermococcus kodakarensis

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.165 6
-
-
 Thermococcus kodakarensis