BRENDA - Enzyme Database show

Purification and characterization of a Bacillus megaterium disulfide reductase specific for disulfides containing pantethine 4',4'-diphosphate

Swerdlow, R.D.; Setlow, P.; J. Bacteriol. 153, 475-484 (1983)

Data extracted from this reference:

Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
1.8.1.8
arsenite
-
Bacillus megaterium
1.8.1.8
N-ethylmaleimide
-
Bacillus megaterium
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
1.8.1.8
0.65
-
pantethine 4',4''-diphosphate
-
Bacillus megaterium
1.8.1.8
7.4
-
CoA-SS-CoA
-
Bacillus megaterium
Molecular Weight [Da]
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
1.8.1.8
55000
-
2 * 55000, SDS-PAGE
Bacillus megaterium
1.8.1.8
122000
-
gel filtration
Bacillus megaterium
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
1.8.1.8
Bacillus megaterium
-
-
-
Purification (Commentary)
EC Number
Commentary
Organism
1.8.1.8
-
Bacillus megaterium
Source Tissue
EC Number
Source Tissue
Commentary
Organism
Textmining
1.8.1.8
cell culture
the enzyme is at a low level in log-phase cells but increases up to 10fold early in the stationary phase and has a similar specific activity in both the mother cell and the forespore compartment. The enzyme activity falls only slowly during spore germination and outgrowth
Bacillus megaterium
-
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1.8.1.8
additional information
the enzyme is active only on disulfides containing pantethine 4',4"-diphosphate moieties, including pantethine 4',4"-diphosphate, oxidized coenzyme A, and coenzyme A in disulfide linkage to acyl carrier protein
680424
Bacillus megaterium
?
-
-
-
-
1.8.1.8
NADH + CoA-SS-CoA + H+
-
680424
Bacillus megaterium
NAD+ + CoA
-
-
-
?
1.8.1.8
NADH + pantethine 4',4''-diphosphate
-
680424
Bacillus megaterium
NAD+ + 4'-phosphopantetheine
-
-
-
?
Subunits
EC Number
Subunits
Commentary
Organism
1.8.1.8
dimer
2 * 55000, SDS-PAGE
Bacillus megaterium
pH Optimum
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
1.8.1.8
6.8
-
substrate: pantethine 4',4''-diphosphate
Bacillus megaterium
pH Range
EC Number
pH Minimum
pH Maximum
Commentary
Organism
1.8.1.8
additional information
-
seven times as active in phosphate buffer as in Tris at the same pH
Bacillus megaterium
Cofactor
EC Number
Cofactor
Commentary
Organism
Structure
1.8.1.8
FAD
contains one molecule of flavin adenine dinucleotide per subunit
Bacillus megaterium
Cofactor (protein specific)
EC Number
Cofactor
Commentary
Organism
Structure
1.8.1.8
FAD
contains one molecule of flavin adenine dinucleotide per subunit
Bacillus megaterium
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
1.8.1.8
arsenite
-
Bacillus megaterium
1.8.1.8
N-ethylmaleimide
-
Bacillus megaterium
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
1.8.1.8
0.65
-
pantethine 4',4''-diphosphate
-
Bacillus megaterium
1.8.1.8
7.4
-
CoA-SS-CoA
-
Bacillus megaterium
Molecular Weight [Da] (protein specific)
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
1.8.1.8
55000
-
2 * 55000, SDS-PAGE
Bacillus megaterium
1.8.1.8
122000
-
gel filtration
Bacillus megaterium
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
1.8.1.8
-
Bacillus megaterium
Source Tissue (protein specific)
EC Number
Source Tissue
Commentary
Organism
Textmining
1.8.1.8
cell culture
the enzyme is at a low level in log-phase cells but increases up to 10fold early in the stationary phase and has a similar specific activity in both the mother cell and the forespore compartment. The enzyme activity falls only slowly during spore germination and outgrowth
Bacillus megaterium
-
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1.8.1.8
additional information
the enzyme is active only on disulfides containing pantethine 4',4"-diphosphate moieties, including pantethine 4',4"-diphosphate, oxidized coenzyme A, and coenzyme A in disulfide linkage to acyl carrier protein
680424
Bacillus megaterium
?
-
-
-
-
1.8.1.8
NADH + CoA-SS-CoA + H+
-
680424
Bacillus megaterium
NAD+ + CoA
-
-
-
?
1.8.1.8
NADH + pantethine 4',4''-diphosphate
-
680424
Bacillus megaterium
NAD+ + 4'-phosphopantetheine
-
-
-
?
Subunits (protein specific)
EC Number
Subunits
Commentary
Organism
1.8.1.8
dimer
2 * 55000, SDS-PAGE
Bacillus megaterium
pH Optimum (protein specific)
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
1.8.1.8
6.8
-
substrate: pantethine 4',4''-diphosphate
Bacillus megaterium
pH Range (protein specific)
EC Number
pH Minimum
pH Maximum
Commentary
Organism
1.8.1.8
additional information
-
seven times as active in phosphate buffer as in Tris at the same pH
Bacillus megaterium