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Literature summary extracted from

  • Latha, G.M.; Muralikrishna, G.
    Purification and partial characterization of acetic acid esterase from malted finger millet (Eleusine coracana, Indaf-15) (2007), J. Agric. Food Chem., 55, 895-902.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
3.1.1.6 Citric acid
-
 Eleusine coracana
3.1.1.6 EDTA
-
 Eleusine coracana

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.1.1.6 Al3+
-
 Eleusine coracana
3.1.1.6 Ba2+
-
 Eleusine coracana
3.1.1.6 Ca2+
-
 Eleusine coracana
3.1.1.6 Co2+
-
 Eleusine coracana
3.1.1.6 eserine competitive, complete inhibition at 25 mM Eleusine coracana
3.1.1.6 Mg2+
-
 Eleusine coracana
3.1.1.6 Mn2+
-
 Eleusine coracana
3.1.1.6 additional information no inhibition by iodoacetamide Eleusine coracana
3.1.1.6 Ni2+
-
 Eleusine coracana
3.1.1.6 PCMB complete inhibition at 25 mM Eleusine coracana
3.1.1.6 Zn2+
-
 Eleusine coracana

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.1.1.6 Cu+ activates Eleusine coracana
3.1.1.6 Cu2+ activates Eleusine coracana
3.1.1.6 Fe3+ activates Eleusine coracana
3.1.1.6 Hg2+ activates Eleusine coracana

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.1.1.6 19700
-
 4 * 19700, SDS-PAGE Eleusine coracana
3.1.1.6 79400
-
 gel filtration Eleusine coracana

Organism

EC Number Organism UniProt Comment Textmining
3.1.1.6 Eleusine coracana
-
 finger millet, also known as ragi, indigenous minor millet in india
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.1.1.6 native enzyme from 72 h ragi malt 34fold by ammonium sulfate precipitation, anion exchange chromatography, gel filtration, and hydrophobic interaction chromatography, to homogeneity Eleusine coracana

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.1.1.6 additional information acetic acid esterase from 72 h ragi malt Eleusine coracana
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.1.1.6 0.027
-
 purified native enzyme, substrate ragi Eleusine coracana
3.1.1.6 0.061
-
 purified native enzyme, substrate larch wood xylan Eleusine coracana
3.1.1.6 0.282
-
 purified native enzyme, substrate gum karaya Eleusine coracana
3.1.1.6 3.38
-
 purified native enzyme, substrate alpha-naphthyl acetate Eleusine coracana
3.1.1.6 4.44
-
 purified native enzyme, substrate wheat Eleusine coracana
3.1.1.6 5.39
-
 purified native enzyme, substrate 4-nitrophenyl acetate Eleusine coracana

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.1.6 1-naphthyl acetate + H2O
-
 Eleusine coracana 1-naphthol + acetate product determination by NMR and mass spectroscopy ?
3.1.1.6 4-nitrophenyl acetate + H2O
-
 Eleusine coracana 4-nitrophenol + acetate product determination by NMR and mass spectroscopy ?
3.1.1.6 additional information the enzyme deacetylates cereal products like water soluble polysaccharides such as ragi, wheat, larch wood xylan, and gum karaya, the enzyme cleaves the acetyl groups substituted at O-2/O-3 of the xylan backbone of arabinoxylans and is known to modulate their functional properties Eleusine coracana ?
-
 ?

Subunits

EC Number Subunits Comment Organism
3.1.1.6 tetramer 4 * 19700, SDS-PAGE Eleusine coracana

Synonyms

EC Number Synonyms Comment Organism
3.1.1.6 acetic acid esterase
-
 Eleusine coracana
3.1.1.6 ragi acetic acid esterase
-
 Eleusine coracana

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.1.1.6 45
-
-
 Eleusine coracana

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.1.1.6 30 40 stable Eleusine coracana

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.1.1.6 7.5
-
-
 Eleusine coracana

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
3.1.1.6 6 9 stable Eleusine coracana