EC Number | Cloned (Comment) | Organism |
---|---|---|
5.4.99.16 | expression in Escherichia coli | Deinococcus radiodurans |
5.4.99.16 | expression in Escherichia coli | Thermus thermophilus |
EC Number | Protein Variants | Comment | Organism |
---|---|---|---|
5.4.99.16 | additional information | deletion mutant lacking 415 amino acids from C-terminus has a lower thermostability and produces more byproducts than wild-type. Fusion protein consisting of Deinococcus radiodurans enzyme N-terminus plus Thermus thermophilus enzyme C-terminus has a higher thermostability than Deinococcus radiodurans wild-type and less byproducts | Thermus thermophilus |
5.4.99.16 | additional information | fusion protein consisting of Deinococcus radiodurans enzyme N-terminus plus Thermus thermophilus enzyme C-terminus has a higher thermostability than Deinococcus radiodurans wild-type and less byproducts | Deinococcus radiodurans |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
5.4.99.16 | Cu2+ | - |
Deinococcus radiodurans | |
5.4.99.16 | Hg2+ | - |
Deinococcus radiodurans | |
5.4.99.16 | Pb2+ | - |
Deinococcus radiodurans | |
5.4.99.16 | Zn2+ | - |
Deinococcus radiodurans |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
5.4.99.16 | 60700 | - |
x * 60700, wild-type, calculated, x * 61000, wild-type, SDS-PAGE, x * 106000, fusion protein of Deinococcus radiodurans enzyme N-terminus plus Thermus thermophilus enzyme C-terminus, SDS-PAGE | Deinococcus radiodurans |
5.4.99.16 | 61000 | - |
x * 106000, wild-type, x * 61000, deletion mutant lacking 415 amino acids from C-terminus, x * 106000, fusion protein of Deinococcus radiodurans enzyme N-terminus plus Thermus thermophilus enzyme C-terminus, SDS-PAGE | Thermus thermophilus |
5.4.99.16 | 61000 | - |
x * 60700, wild-type, calculated, x * 61000, wild-type, SDS-PAGE, x * 106000, fusion protein of Deinococcus radiodurans enzyme N-terminus plus Thermus thermophilus enzyme C-terminus, SDS-PAGE | Deinococcus radiodurans |
5.4.99.16 | 106000 | - |
x * 106000, wild-type, x * 61000, deletion mutant lacking 415 amino acids from C-terminus, x * 106000, fusion protein of Deinococcus radiodurans enzyme N-terminus plus Thermus thermophilus enzyme C-terminus, SDS-PAGE | Thermus thermophilus |
5.4.99.16 | 106000 | - |
x * 60700, wild-type, calculated, x * 61000, wild-type, SDS-PAGE, x * 106000, fusion protein of Deinococcus radiodurans enzyme N-terminus plus Thermus thermophilus enzyme C-terminus, SDS-PAGE | Deinococcus radiodurans |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
5.4.99.16 | Deinococcus radiodurans | - |
- |
- |
5.4.99.16 | Thermus thermophilus | Q7WUI5 | - |
- |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
5.4.99.16 | maltose | - |
Thermus thermophilus | alpha,alpha-trehalose | wild-type, 80% yield | ? | |
5.4.99.16 | maltose | - |
Deinococcus radiodurans | alpha,alpha-trehalose | wild-type, 92% yield | ? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
5.4.99.16 | ? | x * 106000, wild-type, x * 61000, deletion mutant lacking 415 amino acids from C-terminus, x * 106000, fusion protein of Deinococcus radiodurans enzyme N-terminus plus Thermus thermophilus enzyme C-terminus, SDS-PAGE | Thermus thermophilus |
5.4.99.16 | ? | x * 60700, wild-type, calculated, x * 61000, wild-type, SDS-PAGE, x * 106000, fusion protein of Deinococcus radiodurans enzyme N-terminus plus Thermus thermophilus enzyme C-terminus, SDS-PAGE | Deinococcus radiodurans |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
5.4.99.16 | 15 | - |
wild-type | Deinococcus radiodurans |
5.4.99.16 | 40 | - |
both deletion mutant lacking 415 amino acids from C-terminus, and fusion protein of Deinococcus radiodurans enzyme N-terminus plus Thermus thermophilus enzyme C-terminus | Thermus thermophilus |
5.4.99.16 | 40 | - |
fusion protein of Deinococcus radiodurans enzyme N-terminus plus Thermus thermophilus enzyme C-terminus | Deinococcus radiodurans |
5.4.99.16 | 65 | - |
wild-type | Thermus thermophilus |
EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|---|
5.4.99.16 | 60 | - |
30 min, wild-type, almost complete loss of activity, fusion protein consisting of Deinococcus radiodurans enzyme N-terminus plus Thermus thermophilus enzyme C-terminus, 83% residual activity | Deinococcus radiodurans |
5.4.99.16 | 80 | - |
30 min, wild-type, 88% residual activity, fusion protein consisting of Deinococcus radiodurans enzyme N-terminus plus Thermus thermophilus enzyme C-terminus, 83% residual activity | Thermus thermophilus |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
5.4.99.16 | 6.5 | - |
- |
Deinococcus radiodurans |
5.4.99.16 | 6.5 | - |
- |
Thermus thermophilus |
EC Number | pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|---|
5.4.99.16 | 5.5 | 8 | wild-type | Deinococcus radiodurans |
5.4.99.16 | 6 | 9 | - |
Thermus thermophilus |
EC Number | Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|---|
5.4.99.16 | Deinococcus radiodurans | calculated | - |
4.9 |