Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Wang, J.H.; Tsai, M.Y.; Chen, J.J.; Lee, G.C.; Shaw, J.F.
    Role of the C-terminal domain of Thermus thermophilus trehalose synthase in the thermophilicity, thermostability, and efficient production of trehalose (2007), J. Agric. Food Chem., 55, 3435-3443.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
5.4.99.16 expression in Escherichia coli Deinococcus radiodurans
5.4.99.16 expression in Escherichia coli Thermus thermophilus

Protein Variants

EC Number Protein Variants Comment Organism
5.4.99.16 additional information deletion mutant lacking 415 amino acids from C-terminus has a lower thermostability and produces more byproducts than wild-type. Fusion protein consisting of Deinococcus radiodurans enzyme N-terminus plus Thermus thermophilus enzyme C-terminus has a higher thermostability than Deinococcus radiodurans wild-type and less byproducts Thermus thermophilus
5.4.99.16 additional information fusion protein consisting of Deinococcus radiodurans enzyme N-terminus plus Thermus thermophilus enzyme C-terminus has a higher thermostability than Deinococcus radiodurans wild-type and less byproducts Deinococcus radiodurans

Inhibitors

EC Number Inhibitors Comment Organism Structure
5.4.99.16 Cu2+
-
Deinococcus radiodurans
5.4.99.16 Hg2+
-
Deinococcus radiodurans
5.4.99.16 Pb2+
-
Deinococcus radiodurans
5.4.99.16 Zn2+
-
Deinococcus radiodurans

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
5.4.99.16 60700
-
x * 60700, wild-type, calculated, x * 61000, wild-type, SDS-PAGE, x * 106000, fusion protein of Deinococcus radiodurans enzyme N-terminus plus Thermus thermophilus enzyme C-terminus, SDS-PAGE Deinococcus radiodurans
5.4.99.16 61000
-
x * 106000, wild-type, x * 61000, deletion mutant lacking 415 amino acids from C-terminus, x * 106000, fusion protein of Deinococcus radiodurans enzyme N-terminus plus Thermus thermophilus enzyme C-terminus, SDS-PAGE Thermus thermophilus
5.4.99.16 61000
-
x * 60700, wild-type, calculated, x * 61000, wild-type, SDS-PAGE, x * 106000, fusion protein of Deinococcus radiodurans enzyme N-terminus plus Thermus thermophilus enzyme C-terminus, SDS-PAGE Deinococcus radiodurans
5.4.99.16 106000
-
x * 106000, wild-type, x * 61000, deletion mutant lacking 415 amino acids from C-terminus, x * 106000, fusion protein of Deinococcus radiodurans enzyme N-terminus plus Thermus thermophilus enzyme C-terminus, SDS-PAGE Thermus thermophilus
5.4.99.16 106000
-
x * 60700, wild-type, calculated, x * 61000, wild-type, SDS-PAGE, x * 106000, fusion protein of Deinococcus radiodurans enzyme N-terminus plus Thermus thermophilus enzyme C-terminus, SDS-PAGE Deinococcus radiodurans

Organism

EC Number Organism UniProt Comment Textmining
5.4.99.16 Deinococcus radiodurans
-
-
-
5.4.99.16 Thermus thermophilus Q7WUI5
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
5.4.99.16 maltose
-
Thermus thermophilus alpha,alpha-trehalose wild-type, 80% yield ?
5.4.99.16 maltose
-
Deinococcus radiodurans alpha,alpha-trehalose wild-type, 92% yield ?

Subunits

EC Number Subunits Comment Organism
5.4.99.16 ? x * 106000, wild-type, x * 61000, deletion mutant lacking 415 amino acids from C-terminus, x * 106000, fusion protein of Deinococcus radiodurans enzyme N-terminus plus Thermus thermophilus enzyme C-terminus, SDS-PAGE Thermus thermophilus
5.4.99.16 ? x * 60700, wild-type, calculated, x * 61000, wild-type, SDS-PAGE, x * 106000, fusion protein of Deinococcus radiodurans enzyme N-terminus plus Thermus thermophilus enzyme C-terminus, SDS-PAGE Deinococcus radiodurans

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
5.4.99.16 15
-
wild-type Deinococcus radiodurans
5.4.99.16 40
-
both deletion mutant lacking 415 amino acids from C-terminus, and fusion protein of Deinococcus radiodurans enzyme N-terminus plus Thermus thermophilus enzyme C-terminus Thermus thermophilus
5.4.99.16 40
-
fusion protein of Deinococcus radiodurans enzyme N-terminus plus Thermus thermophilus enzyme C-terminus Deinococcus radiodurans
5.4.99.16 65
-
wild-type Thermus thermophilus

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
5.4.99.16 60
-
30 min, wild-type, almost complete loss of activity, fusion protein consisting of Deinococcus radiodurans enzyme N-terminus plus Thermus thermophilus enzyme C-terminus, 83% residual activity Deinococcus radiodurans
5.4.99.16 80
-
30 min, wild-type, 88% residual activity, fusion protein consisting of Deinococcus radiodurans enzyme N-terminus plus Thermus thermophilus enzyme C-terminus, 83% residual activity Thermus thermophilus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
5.4.99.16 6.5
-
-
Deinococcus radiodurans
5.4.99.16 6.5
-
-
Thermus thermophilus

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
5.4.99.16 5.5 8 wild-type Deinococcus radiodurans
5.4.99.16 6 9
-
Thermus thermophilus

pI Value

EC Number Organism Comment pI Value Maximum pI Value
5.4.99.16 Deinococcus radiodurans calculated
-
4.9