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Literature summary extracted from

  • Fang, T.Y.; Tseng, W.C.; Guo, M.S.; Shih, T.Y.; Hung, X.G.
    Expression, purification, and characterization of the maltooligosyltrehalose trehalohydrolase from the thermophilic archaeon Sulfolobus solfataricus ATCC 35092 (2006), J. Agric. Food Chem., 54, 7105-7112.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
3.2.1.141 additional information EDTA does not activate. Expression levels of both wild-type and His-tagged MTHases are higher in the absence of IPTG than in the presence of 0.5 mM IPTG induction Saccharolobus solfataricus

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.2.1.141 pET-15b-deltaH-treZ vector expressed in Escherichia coli BL21-CodonPlus (DE3)-RIL Saccharolobus solfataricus

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.2.1.141 Cu2+ inhibits at 4 mM Saccharolobus solfataricus
3.2.1.141 Hg2+ 0.4 and 4 mM strongly inhibit Saccharolobus solfataricus
3.2.1.141 additional information EDTA, Na+, K+, Mg2+, Ca2+, and Zn2+ do not inhibit Saccharolobus solfataricus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.2.1.141 2.63
-
 maltoheptaose
-
 Saccharolobus solfataricus
3.2.1.141 5.63
-
 maltohexaose
-
 Saccharolobus solfataricus
3.2.1.141 5.66
-
 maltotetraosyl trehalose
-
 Saccharolobus solfataricus
3.2.1.141 5.86
-
 maltopentaose
-
 Saccharolobus solfataricus
3.2.1.141 5.89
-
 maltopentaosyl trehalose
-
 Saccharolobus solfataricus
3.2.1.141 7.22
-
 maltotriosyl trehalose
-
 Saccharolobus solfataricus
3.2.1.141 10.9
-
 maltotetraose
-
 Saccharolobus solfataricus
3.2.1.141 11.1
-
 maltosyl trehalose
-
 Saccharolobus solfataricus

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.2.1.141 additional information Na+, K+, Mg2+, Ca2+, and Zn2+ do not activate Saccharolobus solfataricus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.1.141 64000
-
 2 * 64000, SDS-PAGE, 2 * 64240, electrospray ionization mass spectrometry, wild-type enzyme Saccharolobus solfataricus
3.2.1.141 64240
-
 2 * 64000, SDS-PAGE, 2 * 64240, electrospray ionization mass spectrometry, wild-type enzyme Saccharolobus solfataricus
3.2.1.141 66000
-
 1 * 66000, SDS-PAGE, His-tagged enzyme Saccharolobus solfataricus
3.2.1.141 68700
-
 gel filtration, His-tagged enzyme Saccharolobus solfataricus
3.2.1.141 128800
-
 gel filtration, wild-type enzyme Saccharolobus solfataricus

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.141 Saccharolobus solfataricus P95867 ATCC 35092
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.141 wild-type enzyme purified by heat treatment, nucleic acid precipitation, and ion-exchange chromatography, 12fold. His-tagged MTHase partially purified from the cell-free extract directly by metal chelating chromatography, 14.7fold Saccharolobus solfataricus

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.2.1.141 8 12 wild-type, 12fold purified Saccharolobus solfataricus
3.2.1.141 21.1
-
 His-tagged enzyme, crude extract Saccharolobus solfataricus
3.2.1.141 67.5
-
 wild-type, crude extract Saccharolobus solfataricus
3.2.1.141 310
-
 His-tagged enzyme, 14.7fold purified Saccharolobus solfataricus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.141 maltoheptaose + H2O
-
 Saccharolobus solfataricus ?
-
 ?
3.2.1.141 maltohexaose + H2O for glucose formation, maltohexaose exhibits the highest formation rate Saccharolobus solfataricus ?
-
 ?
3.2.1.141 maltopentaose + H2O
-
 Saccharolobus solfataricus ?
-
 ?
3.2.1.141 maltopentaosyltrehalose + H2O trehalose formation rate increases as the degree of polymerization value of the substrate increases. maltopentaosyl trehalose is the most preferred substrate for trehalose formation Saccharolobus solfataricus maltopentaose + alpha,alpha'-trehalose
-
 ?
3.2.1.141 maltosyltrehalose + H2O
-
 Saccharolobus solfataricus maltose + alpha,alpha'-trehalose
-
 ?
3.2.1.141 maltotetraose + H2O
-
 Saccharolobus solfataricus ?
-
 ?
3.2.1.141 maltotetraosyltrehalose + H2O
-
 Saccharolobus solfataricus maltotetraose + alpha,alpha'-trehalose
-
 ?
3.2.1.141 maltotriosyltrehalose + H2O
-
 Saccharolobus solfataricus maltotriose + alpha,alpha'-trehalose
-
 ?

Subunits

EC Number Subunits Comment Organism
3.2.1.141 homodimer 2 * 64000, SDS-PAGE, 2 * 64240, electrospray ionization mass spectrometry, wild-type enzyme Saccharolobus solfataricus
3.2.1.141 monomer 1 * 66000, SDS-PAGE, His-tagged enzyme Saccharolobus solfataricus

Synonyms

EC Number Synonyms Comment Organism
3.2.1.141 Maltooligosyl trehalose trehalohydrolase
-
 Saccharolobus solfataricus
3.2.1.141 MTHase
-
 Saccharolobus solfataricus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.141 85
-
-
 Saccharolobus solfataricus

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
3.2.1.141 45 85
-
 Saccharolobus solfataricus

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.2.1.141 85
-
 retains all of its activity for 2 h Saccharolobus solfataricus

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.2.1.141 additional information
-
 maltoheptaose
-
 Saccharolobus solfataricus
3.2.1.141 5.49
-
 maltotetraose
-
 Saccharolobus solfataricus
3.2.1.141 6.01
-
 maltoheptaose
-
 Saccharolobus solfataricus
3.2.1.141 17.7
-
 maltopentaose
-
 Saccharolobus solfataricus
3.2.1.141 18.2
-
 maltohexaose
-
 Saccharolobus solfataricus
3.2.1.141 193
-
 maltosyl trehalose
-
 Saccharolobus solfataricus
3.2.1.141 1030
-
 maltotriosyl trehalose
-
 Saccharolobus solfataricus
3.2.1.141 1190
-
 maltotetraosyl trehalose
-
 Saccharolobus solfataricus
3.2.1.141 1230
-
 maltopentaosyl trehalose
-
 Saccharolobus solfataricus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.141 5
-
-
 Saccharolobus solfataricus

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.2.1.141 3.5 11
-
 Saccharolobus solfataricus