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Literature summary extracted from

  • Chen, Y.S.; Lee, G.C.; Shaw, J.F.
    Gene cloning, expression, and biochemical characterization of a recombinant trehalose synthase from Picrophilus torridus in Escherichia coli (2006), J. Agric. Food Chem., 54, 7098-7104.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
5.4.99.16 expression in Escherichia coli Picrophilus torridus

Inhibitors

EC Number Inhibitors Comment Organism Structure
5.4.99.16 Al3+ 1 mM, complete inhibition Picrophilus torridus
5.4.99.16 Hg2+ 1 mM, complete inhibition Picrophilus torridus
5.4.99.16 sodium dodecylsulfate 1 mM, complete inhibition Picrophilus torridus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
5.4.99.16 42.4
-
 maltose 45°C, pH 6.0 Picrophilus torridus
5.4.99.16 137.8
-
 maltose 45°C, pH 6.0, presence of 10 mM glucose Picrophilus torridus
5.4.99.16 210.3
-
 trehalose 45°C, pH 6.0 Picrophilus torridus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
5.4.99.16 65000
-
 x * 65000, SDS-PAGE Picrophilus torridus

Organism

EC Number Organism UniProt Comment Textmining
5.4.99.16 Picrophilus torridus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
5.4.99.16 recombinant enzyme Picrophilus torridus

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
5.4.99.16 80
-
 45°C, pH 6.0 Picrophilus torridus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
5.4.99.16 alpha,alpha-trehalose ratio of kcat to Km value is 2.5fold higher for maltose than for trehalose Picrophilus torridus maltose
-
 r
5.4.99.16 maltose ratio of kcat to Km value is 2.5fold higher for maltose than for trehalose. Maximum conversion rate for maltose into trehalose is independent of substrate concentration and reaches 71% at 20°C Picrophilus torridus alpha,alpha-trehalose
-
 r
5.4.99.16 sucrose low activity on sucrose Picrophilus torridus D-glucose + D-fructose + [alpha-D-glucopyranosyl-(1,1)-D-fructofuranose]
-
 ?

Subunits

EC Number Subunits Comment Organism
5.4.99.16 ? x * 65000, SDS-PAGE Picrophilus torridus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
5.4.99.16 45
-
-
 Picrophilus torridus

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
5.4.99.16 60
-
 pH 6.0, stable for 20 min Picrophilus torridus

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
5.4.99.16 95
-
 maltose 45°C, pH 6.0 Picrophilus torridus
5.4.99.16 97.8
-
 maltose 45°C, pH 6.0, presence of 10 mM glucose Picrophilus torridus
5.4.99.16 187.6
-
 trehalose 45°C, pH 6.0 Picrophilus torridus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
5.4.99.16 6
-
-
 Picrophilus torridus

pH Range

EC Number pH Minimum pH Maximum Comment Organism
5.4.99.16 5 7.5 more than 80% of maximum activity within this range Picrophilus torridus