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Literature summary extracted from

  • Bhatti, H.N.; Asgher, M.; Abbas, A.; Nawaz, R.; Sheikh, M.A.
    Studies on kinetics and thermostability of a novel acid invertase from Fusarium solani (2006), J. Agric. Food Chem., 54, 4617-4623.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.2.1.26 alpha-chymotrypsin 0.5 mg/ml Fusarium solani
3.2.1.26 additional information Pb2+ and Hg2+ do not inhibit the enzyme activity at a concentration of 1 mM Fusarium solani
3.2.1.26 Urea 4 M Fusarium solani

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.2.1.26 3.57
-
 sucrose in MES buffer, pH 5.0, at 40°C Fusarium solani

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.2.1.26 Ba2+ 50% increased activity at 1 mM Fusarium solani
3.2.1.26 Co2+ 69% increased activity at 1 mM Fusarium solani
3.2.1.26 Cu2+ 120% increased activity at 1 mM Fusarium solani
3.2.1.26 K+ 20% increased activity at 1 mM Fusarium solani
3.2.1.26 Mg2+ 105% increased activity at 1 mM Fusarium solani
3.2.1.26 Na+ 60% increased activity at 1 mM Fusarium solani

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.1.26 65000
-
 SDS-PAGE Fusarium solani

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.26 Fusarium solani
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.26 ammonium sulfate fractionation, DEAE-cellulose column chromatography, and Sephadex G-200 gel filtration Fusarium solani

Source Tissue

EC Number Source Tissue Comment Organism Textmining

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.2.1.26 47.92
-
 crude extract, at pH 5.0 and 40 ?C Fusarium solani
3.2.1.26 245.6
-
 after 3.11fold purification, at pH 5.0 and 40 ?C Fusarium solani

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.26 sucrose + H2O
-
 Fusarium solani D-glucose + D-fructose
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.2.1.26 acid invertase
-
 Fusarium solani
3.2.1.26 beta-D-fructofuranosidase
-
 Fusarium solani

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.26 50
-
-
 Fusarium solani

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.26 2.6
-
-
 Fusarium solani

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
3.2.1.26 5
-
 an increase in pH beyond optimum causes a rapid inactivation of the enzyme with 100% loss in activity observed at pH 5.0 Fusarium solani