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Literature summary extracted from

  • Genta, F.A.; Blanes, L.; Cristofoletti, P.T.; do Lago, C.L.; Terra, W.R.; Ferreira, C.
    Purification, characterization and molecular cloning of the major chitinase from Tenebrio molitor larval midgut (2006), Insect Biochem. Mol. Biol., 36, 789-800.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.2.1.14
-
 Tenebrio molitor

General Stability

EC Number General Stability Organism
3.2.1.14 unstable in the presence of midgut proteinases Tenebrio molitor

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.2.1.14 chitobiose
-
 Tenebrio molitor
3.2.1.14 chitotriose
-
 Tenebrio molitor
3.2.1.14 methylumbelliferyl-beta-N',N',N'-triacetyl-chitotrioside
-
 Tenebrio molitor
3.2.1.14 methylumbelliferyl-beta-N',N'-diacetyl-chitobioside
-
 Tenebrio molitor
3.2.1.14 N-(3-dimethylaminopropyl)-N'-ethylcarbodiimide
-
 Tenebrio molitor
3.2.1.14 N-bromosuccinimide
-
 Tenebrio molitor
3.2.1.14 p-hydroxymercuribenzoate
-
 Tenebrio molitor

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.2.1.14 0.014
-
 methylumbelliferyl-beta-N',N'-diacetyl-chitobioside pH 5.0, 30°C Tenebrio molitor
3.2.1.14 0.023
-
 N,N',N'',N''',N''''-pentaacetylchitopentaose pH 5.0, 30°C Tenebrio molitor
3.2.1.14 0.026
-
 methylumbelliferyl-beta-N',N',N'-triacetyl-chitotrioside pH 5.0, 30°C Tenebrio molitor
3.2.1.14 0.05
-
 methylumbelliferyl-beta-N'-acetylglucosamine pH 5.0, 30°C Tenebrio molitor
3.2.1.14 0.18
-
 chitohexaose pH 5.0, 30°C Tenebrio molitor
3.2.1.14 0.98
-
 chitotetraose pH 5.0, 30°C Tenebrio molitor

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.1.14 40000
-
 gel filtration Tenebrio molitor
3.2.1.14 44000
-
 1 * 44000, SDS-PAGE Tenebrio molitor

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.14 Tenebrio molitor
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.14
-
 Tenebrio molitor

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.2.1.14 larva midgut Tenebrio molitor
-
3.2.1.14 midgut larva Tenebrio molitor
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.2.1.14 11.9
-
-
 Tenebrio molitor

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.14 chitin + H2O low activity with colloidal chitin Tenebrio molitor ?
-
 ?
3.2.1.14 chitohexaose + H2O
-
 Tenebrio molitor chitobiose + chitotriose
-
 ?
3.2.1.14 chitopentaose + H2O best substrate Tenebrio molitor chitobiose + chitotriose
-
 ?
3.2.1.14 chitotetraose + H2O
-
 Tenebrio molitor 2 chitobiose
-
 ?
3.2.1.14 methylumbelliferyl-beta-N',N',N'-triacetyl-chitotrioside + H2O
-
 Tenebrio molitor ?
-
 ?
3.2.1.14 methylumbelliferyl-beta-N',N'-diacetyl-chitobioside + H2O
-
 Tenebrio molitor ?
-
 ?
3.2.1.14 methylumbelliferyl-beta-N'-acetylglucosamine + H2O
-
 Tenebrio molitor ?
-
 ?
3.2.1.14 additional information TmChi does not hydrolyze chitobiose, and chitotriose Tenebrio molitor ?
-
 ?
3.2.1.14 N,N',N'',N''',N''''-pentaacetylchitopentaose + 4 H2O
-
 Tenebrio molitor 5 N-acetyl-D-glucosamine
-
 ?

Subunits

EC Number Subunits Comment Organism
3.2.1.14 monomer 1 * 44000, SDS-PAGE Tenebrio molitor

Synonyms

EC Number Synonyms Comment Organism
3.2.1.14 TmChi
-
 Tenebrio molitor

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.2.1.14 300
-
 methylumbelliferyl-beta-N'-acetylglucosamine pH 5.0, 30°C Tenebrio molitor
3.2.1.14 350
-
 methylumbelliferyl-beta-N',N'-diacetyl-chitobioside pH 5.0, 30°C Tenebrio molitor
3.2.1.14 1500
-
 methylumbelliferyl-beta-N',N',N'-triacetyl-chitotrioside pH 5.0, 30°C Tenebrio molitor

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.14 6.5
-
-
 Tenebrio molitor

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
3.2.1.14 0.031
-
 methylumbelliferyl-beta-N',N'-diacetyl-chitobioside pH 5.0, 30°C Tenebrio molitor
3.2.1.14 0.035
-
 methylumbelliferyl-beta-N',N',N'-triacetyl-chitotrioside pH 5.0, 30°C Tenebrio molitor
3.2.1.14 0.04
-
 chitobiose pH 5.0, 30° Tenebrio molitor
3.2.1.14 0.13
-
 chitotriose pH 5.0, 30° Tenebrio molitor