EC Number | Activating Compound | Comment | Organism | Structure |
---|---|---|---|---|
3.4.21.75 | Ca2+ | parasite expresses a Ca2+ dependent, furin-like protease activity | Cryptosporidium parvum |
EC Number | Application | Comment | Organism |
---|---|---|---|
3.4.21.75 | medicine | furin-like protease activity may be involved in mediating host-parasite interactions. Inhibition of infection is due to an effect on the parasite-derived furin activity rather than the host cell furin | Cryptosporidium parvum |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
3.4.21.75 | 1,10-phenanthroline | - |
Cryptosporidium parvum | |
3.4.21.75 | 4-(2-aminoethyl)benzenesulphonyl fluoride | - |
Cryptosporidium parvum | |
3.4.21.75 | decanoyl-Arg-Val-Lys-Arg-chloromethylketone | most inhibitory, inhibits Cryptosporidium parvum infection of HCT-8 cells in a dose-dependent manner, with ca. 50% inhibition occurring at a concentration of 0.01 mM | Cryptosporidium parvum | |
3.4.21.75 | EDTA | 97% inhibition, Ca2+ can reverse the inhibitory effect | Cryptosporidium parvum | |
3.4.21.75 | EDTA | completely inhibits | Homo sapiens | |
3.4.21.75 | EGTA | - |
Cryptosporidium parvum | |
3.4.21.75 | Glu-Gly-Arg-chloromethylketone | poor inhibitor | Cryptosporidium parvum | |
3.4.21.75 | leupeptin | - |
Cryptosporidium parvum | |
3.4.21.75 | additional information | aprotinin has no effect on furin-like protease activity | Cryptosporidium parvum | |
3.4.21.75 | N-tosyl-L-lysine chloromethyl ketone | - |
Cryptosporidium parvum | |
3.4.21.75 | Phe-Pro-Arg-chloromethylketone | poor inhibitor | Cryptosporidium parvum | |
3.4.21.75 | phenylmethylsulfonyl fluoride | - |
Cryptosporidium parvum |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
3.4.21.75 | 0.01517 | - |
t-butyloxycarbonyl-Arg-Val-Arg-Arg-7-amido-4-methylcoumarin | - |
Cryptosporidium parvum |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
3.4.21.75 | Cryptosporidium parvum | - |
- |
- |
3.4.21.75 | Homo sapiens | - |
- |
- |
EC Number | Source Tissue | Comment | Organism | Textmining |
---|---|---|---|---|
3.4.21.75 | HCT-8 cell | - |
Homo sapiens | - |
3.4.21.75 | additional information | lysate | Cryptosporidium parvum | - |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
3.4.21.75 | gp40/15 + H2O | cleaves recombinant Cryptosporidium parvum and Cryptosporidium hominis gp40/15. Putative furin cleavage site RSRR | Homo sapiens | gp40 + gp15 | - |
? | |
3.4.21.75 | gp40/15 + H2O | putative furin cleavage site RSRR | Cryptosporidium parvum | gp40 + gp15 | - |
? | |
3.4.21.75 | gp40/15 subtype 1e + H2O | RSRR sequence is replaced by ISKR, has an alternative furin cleavage site at KSISKR2 | Homo sapiens | gp40 + gp15 | - |
? | |
3.4.21.75 | gp40/15 subtype 1e + H2O | RSRR sequence is replaced by ISKR, has an alternative furin cleavage site at KSISKR2 | Cryptosporidium parvum | gp40 + gp15 | - |
? | |
3.4.21.75 | additional information | mutation in the RSRR cleavage site prevents processing of gp40/15 | Homo sapiens | ? | - |
? | |
3.4.21.75 | additional information | mutation in the RSRR cleavage site prevents processing of gp40/15 | Cryptosporidium parvum | ? | - |
? | |
3.4.21.75 | t-butyloxycarbonyl-Arg-Val-Arg-Arg-7-amido-4-methylcoumarin + H2O | - |
Homo sapiens | ? | - |
? | |
3.4.21.75 | t-butyloxycarbonyl-Arg-Val-Arg-Arg-7-amido-4-methylcoumarin + H2O | - |
Cryptosporidium parvum | ? | - |
? |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
3.4.21.75 | furin-like protease | - |
Cryptosporidium parvum |
3.4.21.75 | subtilisin-like proprotein convertase | - |
Homo sapiens |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
3.4.21.75 | 7 | - |
- |
Cryptosporidium parvum |
EC Number | pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|---|
3.4.21.75 | 4 | 8 | - |
Cryptosporidium parvum |