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Literature summary extracted from

  • Ebel, F.; Schwienbacher, M.; Beyer, J.; Heesemann, J.; Brakhage, A.A.; Brock, M.
    Analysis of the regulation, expression, and localisation of the isocitrate lyase from Aspergillus fumigatus, a potential target for antifungal drug development (2006), Fungal Genet. Biol., 43, 476-489.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
4.1.3.1 3-nitropropionate 0.133 mM, complete inhibition Aspergillus fumigatus
4.1.3.1 succinate 33% inhibition at 10 mM Aspergillus fumigatus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
4.1.3.1 0.51
-
 isocitrate 50 mM MOPS buffer, pH 7.5, with 1 mM MgCl2, 10 mM 2-mercaptoethanol, and 2.7 mM O-nitrophenyl-beta-D-galactopyranoside Aspergillus fumigatus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
4.1.3.1 60230
-
 calculated from amino acid sequence Aspergillus fumigatus
4.1.3.1 61000
-
 SDS-PAGE Aspergillus fumigatus

Organism

EC Number Organism UniProt Comment Textmining
4.1.3.1 Aspergillus fumigatus
-
 strain ATCC46645
-
4.1.3.1 Aspergillus fumigatus ATCC 46645
-
 strain ATCC46645
-

Purification (Commentary)

EC Number Purification (Comment) Organism
4.1.3.1 ammonium sulfate precipitation, phenyl-Sepharose column chromatography, and Resource Q column chromatography Aspergillus fumigatus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
4.1.3.1 mycelium
-
 Aspergillus fumigatus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
4.1.3.1 0.22
-
 crude cell extract Aspergillus fumigatus
4.1.3.1 8.4
-
 after 38.2fold purification Aspergillus fumigatus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.1.3.1 isocitrate
-
 Aspergillus fumigatus succinate + glyoxylate
-
 ?
4.1.3.1 isocitrate
-
 Aspergillus fumigatus ATCC 46645 succinate + glyoxylate
-
 ?

Synonyms

EC Number Synonyms Comment Organism
4.1.3.1 ICL
-
 Aspergillus fumigatus

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
4.1.3.1 8.43
-
 isocitrate in 50 mM MOPS buffer, pH 7.5, with 1 mM MgCl2, 10 mM 2-mercaptoethanol, and 2.7 mM O-nitrophenyl-beta-D-galactopyranoside Aspergillus fumigatus

IC50 Value

EC Number IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
4.1.3.1 5
-
 in 50 mM MOPS buffer, pH 7.5, with 1 mM MgCl2, 10 mM 2-mercaptoethanol, and 2.7 mM O-nitrophenyl-beta-D-galactopyranoside Aspergillus fumigatus succinate