Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Lim, S.; Kim, G.; Yi, S.; Lee, B.H.
    Characteristics of a Bifidobacterium longum LL04 beta-galactosidase (recombinant) produced in Escherichia coli (2006), Food Sci. Biotechnol., 15, 908-913.
No PubMed abstract available

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.2.1.23 expression in Escherichia coli Bifidobacterium longum

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.2.1.23 additional information strong inhibition by most divalent metal ions Bifidobacterium longum

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.2.1.23 0.51
-
 2-nitrophenyl-beta-D-galactopyranoside pH 7.0, 50°C Bifidobacterium longum
3.2.1.23 0.72
-
 2-nitrophenyl-beta-D-galactopyranoside pH 7.0, 37°C Bifidobacterium longum

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.2.1.23 K+ activates Bifidobacterium longum
3.2.1.23 Na+ activates Bifidobacterium longum

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.23 Bifidobacterium longum
-
-
-
3.2.1.23 Bifidobacterium longum LL04
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.23 recombinant enzyme from Escherichia coli partially by ammonium sulfate fractionation and anion exchange chromatography Bifidobacterium longum

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.2.1.23 additional information
-
-
 Bifidobacterium longum

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.23 2-nitrophenyl beta-D-galactopyranoside + H2O
-
 Bifidobacterium longum 2-nitrophenol + beta-D-galactose
-
 ?
3.2.1.23 2-nitrophenyl beta-D-galactopyranoside + H2O
-
 Bifidobacterium longum LL04 2-nitrophenol + beta-D-galactose
-
 ?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.23 50
-
-
 Bifidobacterium longum

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.2.1.23 40
-
 purified recombinant enzyme, over 60 min, pH 7.0, stable Bifidobacterium longum

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.23 7 8
-
 Bifidobacterium longum

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
3.2.1.23 5 9 purified recombinant enzyme, stable Bifidobacterium longum