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Literature summary extracted from

  • Hu, Y.; Morioka, K.; Itoh, Y.
    Hydrolysis of surimi paste from walleye pollock (Theragra chalcogramma) by cysteine proteinase cathepsin L and effect of the proteinase inhibitor (E-64) on gelation (2007), Food Chem., 104, 702-708.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
3.4.22.15 dithiothreitol 325% activity at 2 mM dithiothreitol Gadus chalcogrammus
3.4.22.15 proteinase inhibitor for serine protease 113% activity at 0.1 g/l Gadus chalcogrammus

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.4.22.15 iodoacetate complete inhibition at 1 mM Gadus chalcogrammus
3.4.22.15 L-trans-epoxy-succinyl-leucylamido-(4-guanidino)-butane complete inhibition at 0.1 mM Gadus chalcogrammus
3.4.22.15 leupeptin complete inhibition at 0.1 mM Gadus chalcogrammus
3.4.22.15 additional information not inhibited by EDTA Gadus chalcogrammus

Organism

EC Number Organism UniProt Comment Textmining
3.4.22.15 Gadus chalcogrammus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.4.22.15 Sepharose 6B column chromatography Gadus chalcogrammus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.4.22.15 benzyloxycarbonyl-Arg-7-amido-4-methylcoumarin + H2O
-
 Gadus chalcogrammus benzyloxycarbonyl-Arg + 7-amino-4-methylcoumarin
-
 ?
3.4.22.15 benzyloxycarbonyl-Arg-Arg-7-amido-4-methylcoumarin + H2O
-
 Gadus chalcogrammus benzyloxycarbonyl-Arg-Arg + 7-amino-4-methylcoumarin
-
 ?
3.4.22.15 benzyloxycarbonyl-Phe-Arg-7-amido-4-methylcoumarin + H2O
-
 Gadus chalcogrammus benzyloxycarbonyl-Phe-Arg + 7-amino-4-methylcoumarin
-
 ?
3.4.22.15 myosin heavy chain + H2O
-
 Gadus chalcogrammus ?
-
 ?
3.4.22.15 tert-butyloxycarbonyl-Gln-Ala-Arg-7-amido-4-methylcoumarin + H2O
-
 Gadus chalcogrammus tert-butyloxycarbonyl-Gln-Ala-Arg + 7-amino-4-methylcoumarin
-
 ?

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.4.22.15 25
-
 after incubation at 25°C for 120 min 73-86% of the original activity remain Gadus chalcogrammus

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
3.4.22.15 4 7 at pH 4.0 and 5.0 activity decreases slowly and remains at 38.86% and 48.54%, respectively, after incubation at 45°C for 120 min, at pH 7.0, activity of cathepsin L decreases sharply to about 10% residual activity after incubation at 45°C for 10 min Gadus chalcogrammus