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Literature summary extracted from
- Purification of serine carboxypeptidase from the hepatopancreas of Japanese common squid Todarodes pacificus and its application for elimination of bitterness from bitter peptides (2007), Fish. Sci., 73, 404-411.
Activating Compound
| EC Number | Activating Compound | Comment | Organism | Structure |
|---|---|---|---|---|
| 3.4.16.5 | 1-chloro-3-tosylamido-2-heptanone | 10% increase of activity at 10 mM | Todarodes pacificus |  |
| 3.4.16.5 | antipain | 15 increase of activity at 0.1 mM | Todarodes pacificus | |
| 3.4.16.5 | EDTA | 25% increase of activity at 10 mM | Todarodes pacificus | |
| 3.4.16.5 | leupeptin | 20% increase of activity at 0.1 mM | Todarodes pacificus | |
| 3.4.16.5 | N-ethylmaleimide | 8% increase of activity at 10 mM | Todarodes pacificus | |
| 3.4.16.5 | o-phenanthroline | 4% increase of activity at 5 mM | Todarodes pacificus | |
| 3.4.16.5 | pepstatin A | 5% increase of activity at 0.1 mM | Todarodes pacificus | |
Protein Variants
| EC Number | Protein Variants | Comment | Organism |
|---|---|---|---|
| 3.4.16.5 | nutrition | the enzyme can be used to eliminate the bitterness of bitter peptides | Todarodes pacificus |
Inhibitors
| EC Number | Inhibitors | Comment | Organism | Structure |
|---|---|---|---|---|
| 3.4.16.5 | (S)-1-chloro-4-phenyl-3-tosylamido-2-butanone | 37% residual activity at 1 mM | Todarodes pacificus | |
| 3.4.16.5 | CaCl2 | 73% residual activity at 10 mM | Todarodes pacificus | |
| 3.4.16.5 | chymostatin | 5% residual activity at 1 mM | Todarodes pacificus | |
| 3.4.16.5 | CoSO4 | 62% residual activity at 10 mM | Todarodes pacificus | |
| 3.4.16.5 | CuSO4 | 4% residual activity at 10 mM | Todarodes pacificus | |
| 3.4.16.5 | diisopropylfluorophosphate | complete inhibition at 5 mM, at 30°C and pH 3.1 | Todarodes pacificus | |
| 3.4.16.5 | HgCl2 | complete inhibition at 1 mM, at 30°C and pH 3.1 | Todarodes pacificus | |
| 3.4.16.5 | Monoiodoacetic acid | complete inhibition at 1 mM, at 30°C and pH 3.1 | Todarodes pacificus | |
| 3.4.16.5 | N-(N-[1-trans-3-carboxyoxiran-2-carbonyl]-L-leucyl)-agmatine | 93% residual activity at 1 mM | Todarodes pacificus | |
| 3.4.16.5 | p-chloromercuribenzoic acid | complete inhibition at 1 mM, at 30°C and pH 3.1 | Todarodes pacificus | |
| 3.4.16.5 | Pb(CH3COO)2 | 60% residual activity at 10 mM | Todarodes pacificus | |
| 3.4.16.5 | Phenylmethylsulfonylfluoride | 48% residual activity at 1 mM | Todarodes pacificus | |
| 3.4.16.5 | ZnSO4 | 69% residual activity at 10 mM | Todarodes pacificus | |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 3.4.16.5 | 36000 | - |
SDS-PAGE | Todarodes pacificus |
| 3.4.16.5 | 42000 | - |
gel filtration | Todarodes pacificus |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 3.4.16.5 | Todarodes pacificus | - |
- |
- |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 3.4.16.5 | Mono S cation exchange column chromatography and Superdex 75 gel filtration | Todarodes pacificus |
Source Tissue
| EC Number | Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|---|
| 3.4.16.5 | hepatopancreas | - |
Todarodes pacificus | - |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.4.16.5 | benzyloxycarbonyl-Glu-Phe + H2O | 150% activity compared to benzyloxycarbonyl-Glu-Tyr | Todarodes pacificus | benzyloxycarbonyl-Glu + Phe | - |
? | |
| 3.4.16.5 | Benzyloxycarbonyl-Glu-Tyr + H2O | 100% activity | Todarodes pacificus | Benzyloxycarbonyl-Glu + Tyr | - |
? | |
| 3.4.16.5 | Benzyloxycarbonyl-Gly-Leu + H2O | 17% activity compared to benzyloxycarbonyl-Glu-Tyr | Todarodes pacificus | Benzyloxycarbonyl-Gly + Leu | - |
? | |
| 3.4.16.5 | benzyloxycarbonyl-Gly-Met + H2O | 19% activity compared to benzyloxycarbonyl-Glu-Tyr | Todarodes pacificus | benzyloxycarbonyl-Gly + Met | - |
? | |
| 3.4.16.5 | Benzyloxycarbonyl-Gly-Phe + H2O | 21% activity compared to benzyloxycarbonyl-Glu-Tyr | Todarodes pacificus | Benzyloxycarbonyl-Gly + Phe | - |
? | |
| 3.4.16.5 | benzyloxycarbonyl-Gly-Pro-Leu-Gly + H2O | 309% activity compared to benzyloxycarbonyl-Glu-Tyr | Todarodes pacificus | benzyloxycarbonyl-Gly-Pro-Leu + Gly | - |
? | |
| 3.4.16.5 | benzyloxycarbonyl-Gly-Pro-Leu-Gly-Pro + H2O | 3% activity compared to benzyloxycarbonyl-Glu-Tyr | Todarodes pacificus | benzyloxycarbonyl-Gly-Pro-Leu-Gly + Pro | - |
? | |
| 3.4.16.5 | Benzyloxycarbonyl-Gly-Val + H2O | 24% activity compared to benzyloxycarbonyl-Glu-Tyr | Todarodes pacificus | Benzyloxycarbonyl-Gly + Val | - |
? | |
| 3.4.16.5 | benzyloxycarbonyl-Phe-Ala + H2O | 219% activity compared to benzyloxycarbonyl-Glu-Tyr | Todarodes pacificus | benzyloxycarbonyl-Phe + Ala | - |
? | |
| 3.4.16.5 | benzyloxycarbonyl-Phe-Leu + H2O | 483% activity compared to benzyloxycarbonyl-Glu-Tyr | Todarodes pacificus | benzyloxycarbonyl-Phe + Leu | - |
? | |
| 3.4.16.5 | benzyloxycarbonyl-Phe-Pro + H2O | 12% activity compared to benzyloxycarbonyl-Glu-Tyr | Todarodes pacificus | benzyloxycarbonyl-Phe + Pro | - |
? | |
| 3.4.16.5 | Benzyloxycarbonyl-Phe-Tyr + H2O | 114% activity compared to benzyloxycarbonyl-Glu-Tyr | Todarodes pacificus | Benzyloxycarbonyl-Phe + Tyr | - |
? | |
| 3.4.16.5 | benzyloxycarbonyl-Phe-Tyr-Gly + H2O | 172% activity compared to benzyloxycarbonyl-Glu-Tyr | Todarodes pacificus | benzyloxycarbonyl-Phe-Tyr + Gly | - |
? | |
| 3.4.16.5 | benzyloxycarbonyl-Phe-Tyr-Leu + H2O | 120% activity compared to benzyloxycarbonyl-Glu-Tyr | Todarodes pacificus | benzyloxycarbonyl-Phe-Tyr + Leu | - |
? | |
| 3.4.16.5 | benzyloxycarbonyl-Tyr-Gly + H2O | 188% activity compared to benzyloxycarbonyl-Glu-Tyr | Todarodes pacificus | benzyloxycarbonyl-Tyr + Gly | - |
? | |
| 3.4.16.5 | benzyloxycarbonyl-Tyr-Phe + H2O | 201% activity compared to benzyloxycarbonyl-Glu-Tyr | Todarodes pacificus | benzyloxycarbonyl-Tyr + Phe | - |
? | |
| 3.4.16.5 | corn gluten + H2O | - |
Todarodes pacificus | ? | - |
? | |
| 3.4.16.5 | additional information | no activity towards benzyloxycarbonyl-Gly-Pro, benzoyl-Gly-Lys, and benzyloxycarbonyl-Pro-Pro | Todarodes pacificus | ? | - |
? | |
| 3.4.16.5 | pepsin-digested soy protein + H2O | - |
Todarodes pacificus | ? | - |
? | |
| 3.4.16.5 | trypsin-digested casein + H2O | - |
Todarodes pacificus | ? | - |
? | |
Subunits
| EC Number | Subunits | Comment | Organism |
|---|---|---|---|
| 3.4.16.5 | monomer | 1 * 36000, SDS-PAGE | Todarodes pacificus |
| 3.4.16.5 | monomer | 1 * 42000, gel filtration | Todarodes pacificus |
Synonyms
| EC Number | Synonyms | Comment | Organism |
|---|---|---|---|
| 3.4.16.5 | acidic serine carboxypeptidase | - |
Todarodes pacificus |
| 3.4.16.5 | CPase | - |
Todarodes pacificus |
Temperature Optimum [°C]
| EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 3.4.16.5 | 40 | - |
- |
Todarodes pacificus |
Temperature Stability [°C]
| EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 3.4.16.5 | 45 | 50 | the enzyme activity remained intact at temperatures below 45°C, but declined rapidly at temperatures exceeding 50°C | Todarodes pacificus |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 3.4.16.5 | 4 | - |
- |
Todarodes pacificus |
pH Stability
| EC Number | pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|---|
| 3.4.16.5 | 2 | 6 | at pH 3.1, its relative activity is maintained (70%), the enzyme remains stable in the pH range of 2-6 at 30°C for 30 min | Todarodes pacificus |
pI Value
| EC Number | Organism | Comment | pI Value Maximum | pI Value |
|---|---|---|---|---|
| 3.4.16.5 | Todarodes pacificus | isoelectric focusing | - |
6 |
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