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Literature summary extracted from

  • van Staden, J.; den Haan, R.; van Zyl, W.H.; Botha, A.; Viljoen-Bloom, M.
    Phytase activity in Cryptococcus laurentii ABO 510 (2007), FEMS Yeast Res., 7, 442-448.
    View publication on PubMed

Application

EC Number Application Comment Organism
3.1.3.8 nutrition may be considered for application as an animal feed additive to assist in the hydrolysis of phytate complexes to improve the bioavailability of phosphorus in plant feedstuff Papiliotrema laurentii

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.1.3.8 Ag+ 77.12% residual activity at 5 mM Papiliotrema laurentii
3.1.3.8 Ba2+ 71.95% residual activity at 5 mM Papiliotrema laurentii
3.1.3.8 Ca2+ 68.64% residual activity at 5 mM Papiliotrema laurentii
3.1.3.8 Cd2+ 40.54% residual activity at 1 mM Papiliotrema laurentii
3.1.3.8 Co2+ 76.08% residual activity at 5 mM Papiliotrema laurentii
3.1.3.8 Cu2+ 14.03% residual activity at 5 mM Papiliotrema laurentii
3.1.3.8 Fe2+ 41.3% residual activity at 5 mM Papiliotrema laurentii
3.1.3.8 Hg2+ 31.17% residual activity at 5 mM Papiliotrema laurentii
3.1.3.8 KH2PO4 complete inhibition at 0.5 mM Papiliotrema laurentii
3.1.3.8 Mn2+ 36.01% residual activity at 5 mM Papiliotrema laurentii
3.1.3.8 myo-inositol hexakisphosphate completely inhibited by 5 mM myo-inositol hexakisphosphate Papiliotrema laurentii
3.1.3.8 Ni(2+) 79.25% residual activity at 5 mM Papiliotrema laurentii
3.1.3.8 phosphate completely inhibited by 0.5 mM phosphate Papiliotrema laurentii
3.1.3.8 Zn2+ 27,34% residual activity at 5 mM Papiliotrema laurentii

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.1.3.8 0.021
-
 myo-inositol hexakisphosphate at 62°C, in 50 mM sodium acetate buffer (pH 5) Papiliotrema laurentii
3.1.3.8 0.07
-
 ATP at 62°C, in 50 mM sodium acetate buffer (pH 5) Papiliotrema laurentii
3.1.3.8 0.14
-
 D-fructose 1,6-diphosphate at 62°C, in 50 mM sodium acetate buffer (pH 5) Papiliotrema laurentii
3.1.3.8 0.17
-
 di-sodium phenyl phosphate dihydrate at 62°C, in 50 mM sodium acetate buffer (pH 5) Papiliotrema laurentii
3.1.3.8 0.27
-
 D-glucose 6-phosphate at 62°C, in 50 mM sodium acetate buffer (pH 5) Papiliotrema laurentii
3.1.3.8 0.28
-
 D-glucose 1-phosphate at 62°C, in 50 mM sodium acetate buffer (pH 5) Papiliotrema laurentii
3.1.3.8 0.52
-
 p-nitrophenyl phosphate at 62°C, in 50 mM sodium acetate buffer (pH 5) Papiliotrema laurentii

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.1.3.8 additional information Li+, Na+, K+ and Mg2+ do not significantly affect the phytase activity Papiliotrema laurentii

Organism

EC Number Organism UniProt Comment Textmining
3.1.3.8 Papiliotrema laurentii
-
-
-
3.1.3.8 Papiliotrema laurentii ABO 510
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.3.8 ATP + H2O 76.67% activity compared to myo-inositol hexakisphosphate Papiliotrema laurentii ADP + phosphate
-
 ?
3.1.3.8 ATP + H2O 76.67% activity compared to myo-inositol hexakisphosphate Papiliotrema laurentii ABO 510 ADP + phosphate
-
 ?
3.1.3.8 D-fructose 1,6-diphosphate + H2O 202.19% activity compared to myo-inositol hexakisphosphate Papiliotrema laurentii ?
-
 ?
3.1.3.8 D-fructose 1,6-diphosphate + H2O 202.19% activity compared to myo-inositol hexakisphosphate Papiliotrema laurentii ABO 510 ?
-
 ?
3.1.3.8 D-glucose 1-phosphate + H2O 76.72% activity compared to myo-inositol hexakisphosphate Papiliotrema laurentii D-glucose + phosphate
-
 ?
3.1.3.8 D-glucose 6-phosphate + H2O 73.75% activity compared to myo-inositol hexakisphosphate Papiliotrema laurentii D-glucose + phosphate
-
 ?
3.1.3.8 D-glucose 6-phosphate + H2O 73.75% activity compared to myo-inositol hexakisphosphate Papiliotrema laurentii ABO 510 D-glucose + phosphate
-
 ?
3.1.3.8 di-sodium phenyl phosphate dihydrate + H2O 111.85% activity compared to myo-inositol hexakisphosphate Papiliotrema laurentii ?
-
 ?
3.1.3.8 myo-inositol hexakisphosphate + H2O preferred substrate, 100% activity Papiliotrema laurentii 1-L-myo-inositol 1,2,4,5,6-pentakisphosphate + myo-inositol-1,2,5,6 tetrakisphosphate + phosphate
-
 ?
3.1.3.8 myo-inositol hexakisphosphate + H2O preferred substrate, 100% activity Papiliotrema laurentii ABO 510 1-L-myo-inositol 1,2,4,5,6-pentakisphosphate + myo-inositol-1,2,5,6 tetrakisphosphate + phosphate
-
 ?
3.1.3.8 p-nitrophenyl phosphate + H2O 152.95% activity compared to myo-inositol hexakisphosphate Papiliotrema laurentii p-nitrophenol + phosphate
-
 ?
3.1.3.8 p-nitrophenyl phosphate + H2O 152.95% activity compared to myo-inositol hexakisphosphate Papiliotrema laurentii ABO 510 p-nitrophenol + phosphate
-
 ?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.1.3.8 62
-
-
 Papiliotrema laurentii

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
3.1.3.8 24 80
-
 Papiliotrema laurentii

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.1.3.8 4 70 thermostable between 4°C and 70°C Papiliotrema laurentii
3.1.3.8 70 90 approximately 40% of the original activity is lost after exposure to 70°C for 180 min, with no residual activity being seen after exposure to 80°C for 60 min or 90°C for 5 min Papiliotrema laurentii

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.1.3.8 5
-
-
 Papiliotrema laurentii

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.1.3.8 2.5 7.5
-
 Papiliotrema laurentii