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Literature summary extracted from

  • Takaku, H.; Kimoto, A.; Kodaira, S.; Nashimoto, M.; Takagi, M.
    Isolation of a Gram-positive poly(3-hydroxybutyrate) (PHB)-degrading bacterium from compost, and cloning and characterization of a gene encoding PHB depolymerase of Bacillus megaterium N-18-25-9 (2006), FEMS Microbiol. Lett., 264, 152-159.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.1.1.75 expression in Escherichia coli Priestia megaterium

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.1.1.75 dithiothreitol 70% inhibition at 1 mM and 90% inhibition at 10 mM Priestia megaterium
3.1.1.75 KCl 100 mM, 75% inhibition Priestia megaterium
3.1.1.75 Phenylmethylsulfonylfluoride 10% inhibition at 1 mM and 70% inhibition at 10 mM Priestia megaterium
3.1.1.75 SDS almost complete inhibition at 0.1% Priestia megaterium
3.1.1.75 Triton X-100 almost complete inhibition at 0.1% Priestia megaterium
3.1.1.75 Tween 20 amlnost complete inhibition at 0.1% Priestia megaterium

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.1.1.75 extracellular
-
 Priestia megaterium
-
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.1.1.75 Ca2+ depolymerase activity drastically increases in the presence of 1-100mM Ca2+ Priestia megaterium
3.1.1.75 K+ up to 100 mM, stimulation of depolymerase activity Priestia megaterium
3.1.1.75 Mg2+ up to 10 mM, stimulation of depolymerase activity Priestia megaterium
3.1.1.75 Na+ up to 100 mM, stimulation of depolymerase activity Priestia megaterium

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.1.1.75 poly[(R)-3-hydroxybutanoate]n + H2O Priestia megaterium the addition of PHB to the medium leads to a sixfold increase in PhaZBm mRNA, while the presence of glucose represses phaZBm expression poly[(R)-3-hydroxybutanoate]n-x + poly[(R)-3-hydroxybutanoate]x
-
 ?
3.1.1.75 poly[(R)-3-hydroxybutanoate]n + H2O Priestia megaterium N-18-25-9 the addition of PHB to the medium leads to a sixfold increase in PhaZBm mRNA, while the presence of glucose represses phaZBm expression poly[(R)-3-hydroxybutanoate]n-x + poly[(R)-3-hydroxybutanoate]x
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.1.1.75 Priestia megaterium A0A8W8 N-18-25-9
-
3.1.1.75 Priestia megaterium N-18-25-9 A0A8W8 N-18-25-9
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.1.1.75 expression in Escherichia coli Priestia megaterium

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.1.75 p-nitrophenyl acetate + H2O
-
 Priestia megaterium p-nitrophenol + acetate
-
 ?
3.1.1.75 p-nitrophenyl acetate + H2O
-
 Priestia megaterium N-18-25-9 p-nitrophenol + acetate
-
 ?
3.1.1.75 p-nitrophenyl butyrate + H2O
-
 Priestia megaterium p-nitrophenol + butyrate
-
 ?
3.1.1.75 p-nitrophenyl butyrate + H2O
-
 Priestia megaterium N-18-25-9 p-nitrophenol + butyrate
-
 ?
3.1.1.75 poly[(R)-3-hydroxybutanoate]n + H2O the addition of PHB to the medium leads to a sixfold increase in PhaZBm mRNA, while the presence of glucose represses phaZBm expression Priestia megaterium poly[(R)-3-hydroxybutanoate]n-x + poly[(R)-3-hydroxybutanoate]x
-
 ?
3.1.1.75 poly[(R)-3-hydroxybutanoate]n + H2O the addition of PHB to the medium leads to a sixfold increase in PhaZBm mRNA, while the presence of glucose represses phaZBm expression Priestia megaterium N-18-25-9 poly[(R)-3-hydroxybutanoate]n-x + poly[(R)-3-hydroxybutanoate]x
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.1.1.75 PhaZBm
-
 Priestia megaterium

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.1.1.75 65
-
-
 Priestia megaterium

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
3.1.1.75 50 75 50°C: about 50% of maximal activity, 75°C: about 50% of maximal activity Priestia megaterium

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.1.1.75 9
-
-
 Priestia megaterium

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.1.1.75 8 10 pH 8.0: 70% of maximal activity, pH 10.0: about 85% of maximal activity Priestia megaterium