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Literature summary extracted from

  • Hayashi, K.; Kawaide, H.; Notomi, M.; Sakigi, Y.; Matsuo, A.; Nozaki, H.
    Identification and functional analysis of bifunctional ent-kaurene synthase from the moss Physcomitrella patens (2006), FEBS Lett., 580, 6175-6181.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
4.2.3.19 into the pTD1 vector as a fusion of copalyl diphosphate synthase and ent-kaurene synthase for in vitro expression Physcomitrium patens
5.5.1.13 expression as myc-fusion protein using Spodoptera frugiperda 21 insect cells. Sequence contains a DVDD motif responsible for copalyl diphosphate synthase activity and a DDYFD motif responsible for ent-kaurene synthase activity Physcomitrium patens

Protein Variants

EC Number Protein Variants Comment Organism
4.2.3.19 D635A/D636A mutation of the DDYFD motif in the kaurene synthase domain Physcomitrium patens
5.5.1.13 additional information sequence contains a DVDD motif responsible for copalyl diphosphate synthase activity and a DDYFD motif responsible for ent-kaurene synthase activity. Mutation of DVDD motif to AVAD leads to loss of function, mutation of DDYFD motif to AAYFD results in accumulation of ent-copalyl diphosphate Physcomitrium patens

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
4.2.3.19 106000
-
 copalyl diphosphate synthase-kaurene synthase fusion protein, determined by SDS-PAGE and Western blot analysis Physcomitrium patens
5.5.1.13 101000
-
 x * 101000, calculated, x * 106000, SDs-PAGE of myc-fusion protein Physcomitrium patens
5.5.1.13 106000
-
 x * 101000, calculated, x * 106000, SDs-PAGE of myc-fusion protein Physcomitrium patens

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
4.2.3.19 ent-copalyl diphosphate Physcomitrium patens
-
 ent-kaurene + diphosphate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
4.2.3.19 Physcomitrium patens A5A8G0
-
-
5.5.1.13 Physcomitrium patens
-
 bifunctional copalyl diphosphate synthase/ent-kaurene synthase
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.2.3.19 ent-copalyl diphosphate
-
 Physcomitrium patens ent-kaurene + diphosphate
-
 ?
4.2.3.19 geranylgeranyl diphosphate PpCPS-KS directly synthesizes the ent-kaurene skeleton from GGDP Physcomitrium patens ent-kaurene + diphosphate
-
 ?
5.5.1.13 copalyl diphosphate bifunctional copalyl diphosphate synthase/ent-kaurene synthase Physcomitrium patens ent-16-alpha-hydroxy-kaurene + ent-kaurene + diphosphate 86% and 14%, resp. ?
5.5.1.13 geranylgeranyl diphosphate
-
 Physcomitrium patens copalyl diphosphate
-
 ?

Subunits

EC Number Subunits Comment Organism
5.5.1.13 ? x * 101000, calculated, x * 106000, SDs-PAGE of myc-fusion protein Physcomitrium patens

Synonyms

EC Number Synonyms Comment Organism
4.2.3.19 ent-kaurene synthase
-
 Physcomitrium patens
4.2.3.19 KS
-
 Physcomitrium patens
4.2.3.19 PpCPS/KS bifunctional cyclase with copalyl disphosphatase and kaurene synthase activity Physcomitrium patens
4.2.3.19 PpKS
-
 Physcomitrium patens