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Literature summary extracted from
- Characterization of Mycobacterium tuberculosis nicotinamidase/pyrazinamidase (2008), FEBS J., 275, 753-762.
Application
| EC Number | Application | Comment | Organism |
|---|---|---|---|
| 3.5.1.19 | drug development | enzyme is involved in the activation of the antituberculosis drug pyrazinamide | Mycobacterium tuberculosis |
Cloned(Commentary)
| EC Number | Cloned (Comment) | Organism |
|---|---|---|
| 3.5.1.19 | expression in Escherichia coli | Mycobacterium tuberculosis |
Protein Variants
| EC Number | Protein Variants | Comment | Organism |
|---|---|---|---|
| 3.5.1.19 | C138A | significant decrease in enzyme activity | Mycobacterium tuberculosis |
| 3.5.1.19 | D49A | significant decrease in enzyme activity | Mycobacterium tuberculosis |
| 3.5.1.19 | D8A | significant decrease in enzyme activity | Mycobacterium tuberculosis |
| 3.5.1.19 | H51A | significant decrease in enzyme activity | Mycobacterium tuberculosis |
| 3.5.1.19 | H57A | significant decrease in enzyme activity | Mycobacterium tuberculosis |
| 3.5.1.19 | H71A | significant decrease in enzyme activity | Mycobacterium tuberculosis |
| 3.5.1.19 | K96A | significant decrease in enzyme activity | Mycobacterium tuberculosis |
| 3.5.1.19 | S104A | partial loss of enzyme activity | Mycobacterium tuberculosis |
| 3.5.1.19 | S95A | partial loss of enzyme activity | Mycobacterium tuberculosis |
Metals/Ions
| EC Number | Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|---|
| 3.5.1.19 | Fe2+ | - |
Mycobacterium tuberculosis |  |
| 3.5.1.19 | Mn2+ | - |
Mycobacterium tuberculosis | |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 3.5.1.19 | 20890 | - |
determined by analytical ultracentrifugation and mass spectrometry | Mycobacterium tuberculosis |
Natural Substrates/ Products (Substrates)
| EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.5.1.19 | nicotinamide + H2O | Mycobacterium tuberculosis | - |
NH3 + nicotinic acid | - |
? | |
| 3.5.1.19 | pyrazinamide + H2O | Mycobacterium tuberculosis | - |
NH3 + pyrazinoic acid | - |
? | |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 3.5.1.19 | Mycobacterium tuberculosis | - |
- |
- |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 3.5.1.19 | nickel chelate chromatography and molecular sieve | Mycobacterium tuberculosis |
Specific Activity [micromol/min/mg]
| EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|---|
| 3.5.1.19 | 81.9 | - |
- |
Mycobacterium tuberculosis |
| 3.5.1.19 | 89.6 | - |
- |
Mycobacterium tuberculosis |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.5.1.19 | nicotinamide + H2O | - |
Mycobacterium tuberculosis | NH3 + nicotinic acid | - |
? | |
| 3.5.1.19 | pyrazinamide + H2O | - |
Mycobacterium tuberculosis | NH3 + pyrazinoic acid | - |
? | |
Subunits
| EC Number | Subunits | Comment | Organism |
|---|---|---|---|
| 3.5.1.19 | monomer | - |
Mycobacterium tuberculosis |
Synonyms
| EC Number | Synonyms | Comment | Organism |
|---|---|---|---|
| 3.5.1.19 | nicotinamidase/pyrazinamidase | - |
Mycobacterium tuberculosis |
| 3.5.1.19 | PncA | - |
Mycobacterium tuberculosis |
Temperature Optimum [°C]
| EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 3.5.1.19 | 40 | - |
- |
Mycobacterium tuberculosis |
Temperature Stability [°C]
| EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 3.5.1.19 | 25 | 70 | - |
Mycobacterium tuberculosis |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 3.5.1.19 | 7 | - |
- |
Mycobacterium tuberculosis |
pH Stability
| EC Number | pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|---|
| 3.5.1.19 | 6 | 8 | - |
Mycobacterium tuberculosis |
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Release 2023.1 (January 2023)
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