BRENDA - Enzyme Database

Tissue expression and biochemical characterization of human 2-amino 3-carboxymuconate 6-semialdehyde decarboxylase, a key enzyme in tryptophan catabolism

Pucci, L.; Perozzi, S.; Cimadamore, F.; Orsomando, G.; Raffaelli, N.; FEBS J. 274, 827-840 (2007)

Data extracted from this reference:

Application
EC Number
Application
Commentary
Organism
4.1.1.45
medicine
the enzyme is an therapeutic target for treating disorders associated with increased levels of tryptophan metabolites
Homo sapiens
Cloned(Commentary)
EC Number
Commentary
Organism
4.1.1.45
expressed in Pichia pastoris GS115 cells
Homo sapiens
Engineering
EC Number
Amino acid exchange
Commentary
Organism
4.1.1.45
H6A
activity decreases by about 82%
Homo sapiens
4.1.1.45
H8A
activity decreases by about 50%
Homo sapiens
Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
4.1.1.45
Cd2+
0.5 mM, reduces the enzymatic activity to 25%
Homo sapiens
4.1.1.45
Cr3+
0.5 mM, reduces the enzymatic activity to 35%
Homo sapiens
4.1.1.45
Fe3+
0.1 mM, reduces the enzymatic activity to 15%
Homo sapiens
4.1.1.45
Kynurenic acid
1 mM, 59% residual activity
Homo sapiens
4.1.1.45
Picolinic acid
1 mM, 47% residual activity
Homo sapiens
4.1.1.45
quinolinic acid
1 mM, 61% residual activity
Homo sapiens
4.1.1.45
Zn2+
0.1 mM, reduces the enzymatic activity to 6%
Homo sapiens
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
4.1.1.45
0.0065
-
2-amino-3-(3-oxoprop-2-enyl)-but-2-enedioate
in 50 mM 4-morpholinepropanesulfonic acid, pH 6.0, at 25°C
Homo sapiens
Metals/Ions
EC Number
Metals/Ions
Commentary
Organism
Structure
4.1.1.45
Co2+
1.0 mM, increases activity to 130%
Homo sapiens
4.1.1.45
Fe2+
0.1 mM, increases activity to 124%
Homo sapiens
4.1.1.45
additional information
not influenced by 1 mM Mg2+, Mn2+, Ni2+ or Ca2+; the pure enzyme is 100% active in the absence of any metal ion
Homo sapiens
Molecular Weight [Da]
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
4.1.1.45
40000
-
SDS-PAGE
Homo sapiens
4.1.1.45
50000
-
gel filtration
Homo sapiens
Natural Substrates/ Products (Substrates)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
4.1.1.45
additional information
Homo sapiens
plays a key role in tryptophan catabolism, the enzyme regulates NAD biosynthesis from the amino acid, directly affecting quinolinate and picolinate formation
?
-
-
-
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
4.1.1.45
Homo sapiens
-
-
-
Purification (Commentary)
EC Number
Commentary
Organism
4.1.1.45
hydroxyapatite column chromatography, MonoQ column chromatography and Superose 12 gel filtration
Homo sapiens
Source Tissue
EC Number
Source Tissue
Commentary
Organism
Textmining
4.1.1.45
brain
-
Homo sapiens
-
4.1.1.45
kidney
-
Homo sapiens
-
4.1.1.45
liver
-
Homo sapiens
-
Specific Activity [micromol/min/mg]
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
4.1.1.45
0.006
-
crude cell extract, in 50 mM 4-morpholinepropanesulfonic acid, pH 6.0, at 25°C
Homo sapiens
4.1.1.45
1.39
-
after 231fold purification, in 50 mM 4-morpholinepropanesulfonic acid, pH 6.0, at 25°C
Homo sapiens
Storage Stability
EC Number
Storage Stability
Organism
4.1.1.45
4°C, 10 mM Tris-HCl, pH 8.0, 0.13 m NaCl, several weeks, remains stable
Homo sapiens
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
4.1.1.45
2-amino-3-(3-oxoprop-2-enyl)-but-2-enedioate
-
679800
Homo sapiens
2-aminomuconate-6-semialdehyde + CO2
-
-
-
?
4.1.1.45
additional information
plays a key role in tryptophan catabolism, the enzyme regulates NAD biosynthesis from the amino acid, directly affecting quinolinate and picolinate formation
679800
Homo sapiens
?
-
-
-
-
Subunits
EC Number
Subunits
Commentary
Organism
4.1.1.45
monomer
-
Homo sapiens
Temperature Stability [°C]
EC Number
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
4.1.1.45
-20
-
the purified protein is sensitive to freezing at -20°C
Homo sapiens
Turnover Number [1/s]
EC Number
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
4.1.1.45
1
-
2-amino-3-(3-oxoprop-2-enyl)-but-2-enedioate
in 50 mM 4-morpholinepropanesulfonic acid, pH 6.0, at 25°C
Homo sapiens
pH Optimum
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
4.1.1.45
6.5
8
-
Homo sapiens
Application (protein specific)
EC Number
Application
Commentary
Organism
4.1.1.45
medicine
the enzyme is an therapeutic target for treating disorders associated with increased levels of tryptophan metabolites
Homo sapiens
Cloned(Commentary) (protein specific)
EC Number
Commentary
Organism
4.1.1.45
expressed in Pichia pastoris GS115 cells
Homo sapiens
Engineering (protein specific)
EC Number
Amino acid exchange
Commentary
Organism
4.1.1.45
H6A
activity decreases by about 82%
Homo sapiens
4.1.1.45
H8A
activity decreases by about 50%
Homo sapiens
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
4.1.1.45
Cd2+
0.5 mM, reduces the enzymatic activity to 25%
Homo sapiens
4.1.1.45
Cr3+
0.5 mM, reduces the enzymatic activity to 35%
Homo sapiens
4.1.1.45
Fe3+
0.1 mM, reduces the enzymatic activity to 15%
Homo sapiens
4.1.1.45
Kynurenic acid
1 mM, 59% residual activity
Homo sapiens
4.1.1.45
Picolinic acid
1 mM, 47% residual activity
Homo sapiens
4.1.1.45
quinolinic acid
1 mM, 61% residual activity
Homo sapiens
4.1.1.45
Zn2+
0.1 mM, reduces the enzymatic activity to 6%
Homo sapiens
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
4.1.1.45
0.0065
-
2-amino-3-(3-oxoprop-2-enyl)-but-2-enedioate
in 50 mM 4-morpholinepropanesulfonic acid, pH 6.0, at 25°C
Homo sapiens
Metals/Ions (protein specific)
EC Number
Metals/Ions
Commentary
Organism
Structure
4.1.1.45
Co2+
1.0 mM, increases activity to 130%
Homo sapiens
4.1.1.45
Fe2+
0.1 mM, increases activity to 124%
Homo sapiens
4.1.1.45
additional information
not influenced by 1 mM Mg2+, Mn2+, Ni2+ or Ca2+; the pure enzyme is 100% active in the absence of any metal ion
Homo sapiens
Molecular Weight [Da] (protein specific)
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
4.1.1.45
40000
-
SDS-PAGE
Homo sapiens
4.1.1.45
50000
-
gel filtration
Homo sapiens
Natural Substrates/ Products (Substrates) (protein specific)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
4.1.1.45
additional information
Homo sapiens
plays a key role in tryptophan catabolism, the enzyme regulates NAD biosynthesis from the amino acid, directly affecting quinolinate and picolinate formation
?
-
-
-
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
4.1.1.45
hydroxyapatite column chromatography, MonoQ column chromatography and Superose 12 gel filtration
Homo sapiens
Source Tissue (protein specific)
EC Number
Source Tissue
Commentary
Organism
Textmining
4.1.1.45
brain
-
Homo sapiens
-
4.1.1.45
kidney
-
Homo sapiens
-
4.1.1.45
liver
-
Homo sapiens
-
Specific Activity [micromol/min/mg] (protein specific)
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
4.1.1.45
0.006
-
crude cell extract, in 50 mM 4-morpholinepropanesulfonic acid, pH 6.0, at 25°C
Homo sapiens
4.1.1.45
1.39
-
after 231fold purification, in 50 mM 4-morpholinepropanesulfonic acid, pH 6.0, at 25°C
Homo sapiens
Storage Stability (protein specific)
EC Number
Storage Stability
Organism
4.1.1.45
4°C, 10 mM Tris-HCl, pH 8.0, 0.13 m NaCl, several weeks, remains stable
Homo sapiens
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
4.1.1.45
2-amino-3-(3-oxoprop-2-enyl)-but-2-enedioate
-
679800
Homo sapiens
2-aminomuconate-6-semialdehyde + CO2
-
-
-
?
4.1.1.45
additional information
plays a key role in tryptophan catabolism, the enzyme regulates NAD biosynthesis from the amino acid, directly affecting quinolinate and picolinate formation
679800
Homo sapiens
?
-
-
-
-
Subunits (protein specific)
EC Number
Subunits
Commentary
Organism
4.1.1.45
monomer
-
Homo sapiens
Temperature Stability [°C] (protein specific)
EC Number
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
4.1.1.45
-20
-
the purified protein is sensitive to freezing at -20°C
Homo sapiens
Turnover Number [1/s] (protein specific)
EC Number
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
4.1.1.45
1
-
2-amino-3-(3-oxoprop-2-enyl)-but-2-enedioate
in 50 mM 4-morpholinepropanesulfonic acid, pH 6.0, at 25°C
Homo sapiens
pH Optimum (protein specific)
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
4.1.1.45
6.5
8
-
Homo sapiens