Any feedback?
Literature summary extracted from
- Tissue expression and biochemical characterization of human 2-amino 3-carboxymuconate 6-semialdehyde decarboxylase, a key enzyme in tryptophan catabolism (2007), FEBS J., 274, 827-840.
Application
| EC Number | Application | Comment | Organism |
|---|---|---|---|
| 4.1.1.45 | medicine | the enzyme is an therapeutic target for treating disorders associated with increased levels of tryptophan metabolites | Homo sapiens |
Cloned(Commentary)
| EC Number | Cloned (Comment) | Organism |
|---|---|---|
| 4.1.1.45 | expressed in Pichia pastoris GS115 cells | Homo sapiens |
Protein Variants
| EC Number | Protein Variants | Comment | Organism |
|---|---|---|---|
| 4.1.1.45 | H6A | activity decreases by about 82% | Homo sapiens |
| 4.1.1.45 | H8A | activity decreases by about 50% | Homo sapiens |
Inhibitors
| EC Number | Inhibitors | Comment | Organism | Structure |
|---|---|---|---|---|
| 4.1.1.45 | Cd2+ | 0.5 mM, reduces the enzymatic activity to 25% | Homo sapiens |  |
| 4.1.1.45 | Cr3+ | 0.5 mM, reduces the enzymatic activity to 35% | Homo sapiens | |
| 4.1.1.45 | Fe3+ | 0.1 mM, reduces the enzymatic activity to 15% | Homo sapiens | |
| 4.1.1.45 | Kynurenic acid | 1 mM, 59% residual activity | Homo sapiens | |
| 4.1.1.45 | Picolinic acid | 1 mM, 47% residual activity | Homo sapiens | |
| 4.1.1.45 | quinolinic acid | 1 mM, 61% residual activity | Homo sapiens | |
| 4.1.1.45 | Zn2+ | 0.1 mM, reduces the enzymatic activity to 6% | Homo sapiens | |
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 4.1.1.45 | 0.0065 | - |
2-amino-3-(3-oxoprop-2-enyl)-but-2-enedioate | in 50 mM 4-morpholinepropanesulfonic acid, pH 6.0, at 25°C | Homo sapiens | |
Metals/Ions
| EC Number | Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|---|
| 4.1.1.45 | Co2+ | 1.0 mM, increases activity to 130% | Homo sapiens | |
| 4.1.1.45 | Fe2+ | 0.1 mM, increases activity to 124% | Homo sapiens | |
| 4.1.1.45 | additional information | not influenced by 1 mM Mg2+, Mn2+, Ni2+ or Ca2+ | Homo sapiens | |
| 4.1.1.45 | additional information | the pure enzyme is 100% active in the absence of any metal ion | Homo sapiens |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 4.1.1.45 | 40000 | - |
SDS-PAGE | Homo sapiens |
| 4.1.1.45 | 50000 | - |
gel filtration | Homo sapiens |
Natural Substrates/ Products (Substrates)
| EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 4.1.1.45 | additional information | Homo sapiens | plays a key role in tryptophan catabolism, the enzyme regulates NAD biosynthesis from the amino acid, directly affecting quinolinate and picolinate formation | ? | - |
? | |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 4.1.1.45 | Homo sapiens | - |
- |
- |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 4.1.1.45 | hydroxyapatite column chromatography, MonoQ column chromatography and Superose 12 gel filtration | Homo sapiens |
Source Tissue
| EC Number | Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|---|
| 4.1.1.45 | brain | - |
Homo sapiens | - |
| 4.1.1.45 | kidney | - |
Homo sapiens | - |
| 4.1.1.45 | liver | - |
Homo sapiens | - |
Specific Activity [micromol/min/mg]
| EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|---|
| 4.1.1.45 | 0.006 | - |
crude cell extract, in 50 mM 4-morpholinepropanesulfonic acid, pH 6.0, at 25°C | Homo sapiens |
| 4.1.1.45 | 1.39 | - |
after 231fold purification, in 50 mM 4-morpholinepropanesulfonic acid, pH 6.0, at 25°C | Homo sapiens |
Storage Stability
| EC Number | Storage Stability | Organism |
|---|---|---|
| 4.1.1.45 | 4°C, 10 mM Tris-HCl, pH 8.0, 0.13 m NaCl, several weeks, remains stable | Homo sapiens |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 4.1.1.45 | 2-amino-3-(3-oxoprop-2-enyl)-but-2-enedioate | - |
Homo sapiens | 2-aminomuconate-6-semialdehyde + CO2 | - |
? | |
| 4.1.1.45 | additional information | plays a key role in tryptophan catabolism, the enzyme regulates NAD biosynthesis from the amino acid, directly affecting quinolinate and picolinate formation | Homo sapiens | ? | - |
? | |
Subunits
| EC Number | Subunits | Comment | Organism |
|---|---|---|---|
| 4.1.1.45 | monomer | - |
Homo sapiens |
Synonyms
| EC Number | Synonyms | Comment | Organism |
|---|---|---|---|
| 4.1.1.45 | 2-amino 3-carboxymuconate 6-semialdehyde decarboxylase | - |
Homo sapiens |
| 4.1.1.45 | ACMS decarboxylase | - |
Homo sapiens |
| 4.1.1.45 | ACMSD I | enzymatically active form | Homo sapiens |
Temperature Stability [°C]
| EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 4.1.1.45 | -20 | - |
the purified protein is sensitive to freezing at -20°C | Homo sapiens |
Turnover Number [1/s]
| EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 4.1.1.45 | 1 | - |
2-amino-3-(3-oxoprop-2-enyl)-but-2-enedioate | in 50 mM 4-morpholinepropanesulfonic acid, pH 6.0, at 25°C | Homo sapiens | |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 4.1.1.45 | 6.5 | 8 | - |
Homo sapiens |
Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.
Release 2023.1 (January 2023)
Legal
Curated at
Member of
