Literature summary extracted from

Chiaraluce, R.; Florio, R.; Angelaccio, S.; Gianese, G.; van Lieshout, J.F.; van der Oost, J.; Consalvi, V.
Tertiary structure in 7.9 M guanidinium chloride--the role of Glu53 and Asp287 in Pyrococcus furiosus endo-beta-1,3-glucanase (2007), FEBS J., 274, 6167-6179.

Cloned(Commentary)

EC Number	Cloned (Comment)	Organism
3.2.1.39	expressed in Escherichia coli strain BL21(DE3)	Pyrococcus furiosus

Protein Variants

EC Number	Protein Variants	Comment	Organism
3.2.1.39	D287A	decreased activity	Pyrococcus furiosus
3.2.1.39	E53A	decreased activity	Pyrococcus furiosus
3.2.1.39	E53A/D287A	decreased activity	Pyrococcus furiosus

Organism

EC Number	Organism	UniProt	Comment	Textmining
3.2.1.39	Pyrococcus furiosus	-	-	-

Purification (Commentary)

EC Number	Purification (Comment)	Organism
3.2.1.39	-	Pyrococcus furiosus

Renatured (Commentary)

EC Number	Renatured (Comment)	Organism
3.2.1.39	refolding is started by 20fold dilution of the unfolding mixture, at 20°C, into solutions of buffer containing decreasing guanidinium chloride concentrations	Pyrococcus furiosus

Substrates and Products (Substrate)

EC Number	Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
3.2.1.39	beta-glucan + H2O	-	Pyrococcus furiosus	?	-	?
3.2.1.39	laminarin + H2O	-	Pyrococcus furiosus	D-glucose + ?	-	?

Synonyms

EC Number	Synonyms	Comment	Organism
3.2.1.39	endo-beta-1,3-glucanase	-	Pyrococcus furiosus

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Release 2023.1 (January 2023)