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Literature summary extracted from

  • Ludwig, H.C.; Pardo, F.N.; Asenjo, J.L.; Maureira, M.A.; Yanez, A.J.; Slebe, J.C.
    Unraveling multistate unfolding of pig kidney fructose-1,6-bisphosphatase using single tryptophan mutants (2007), FEBS J., 274, 5337-5349.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.1.3.11
-
 Sus scrofa

Protein Variants

EC Number Protein Variants Comment Organism
3.1.3.11 F16W mutant FBPases exhibits identical electrophoretic mobility as FBPase isolated from pig kidney. Mutation does not affect catalytic properties significantly, except the loss of AMP cooperativity Sus scrofa
3.1.3.11 F219W mutant FBPases exhibits identical electrophoretic mobility as FBPase isolated from pig kidney. Mutation does not affect catalytic properties significantly Sus scrofa
3.1.3.11 F232W mutant FBPases exhibits identical electrophoretic mobility as FBPase isolated from pig kidney. Mutation does not affect catalytic properties significantly Sus scrofa
3.1.3.11 F89W mutant FBPases exhibits identical electrophoretic mobility as FBPase isolated from pig kidney. Mutation does not affect catalytic properties significantly Sus scrofa

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.1.3.11 AMP
-
 Sus scrofa
3.1.3.11 D-fructose 2,6-bisphosphate
-
 Sus scrofa

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.1.3.11 0.0043
-
 D-fructose 1,6-bisphosphate 30°C, pH 7.5, mutant enzyme F89W Sus scrofa
3.1.3.11 0.0046
-
 D-fructose 1,6-bisphosphate 30°C, pH 7.5, mutant enzyme F232W Sus scrofa
3.1.3.11 0.0048
-
 D-fructose 1,6-bisphosphate 30°C, pH 7.5, recombinant wild-type enzyme Sus scrofa
3.1.3.11 0.005
-
 D-fructose 1,6-bisphosphate 30°C, pH 7.5, mutant enzyme F16W Sus scrofa
3.1.3.11 0.0059
-
 D-fructose 1,6-bisphosphate 30°C, pH 7.5, nonrecombinant enzyme Sus scrofa
3.1.3.11 0.006
-
 D-fructose 1,6-bisphosphate 30°C, pH 7.5, mutant enzyme F219W Sus scrofa

Organism

EC Number Organism UniProt Comment Textmining
3.1.3.11 Sus scrofa P00636
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.1.3.11
-
 Sus scrofa

Renatured (Commentary)

EC Number Renatured (Comment) Organism
3.1.3.11 the unfolded FBPase mutants in 3.5 M guanidinium chloride are diluted to a concentration of 0.1 M guanidinium chloride the recoveries of enzymatic activity are as follows: 60.9% for F16W FBPase, 57.2% for F89W FBPase, 59.8% for F219W FBPase, and 63.6% for F232W FBPase. These results indicate that the unfolding process is not completely reversible. The reduced reversibility is similar to that observed for nonrecombinant FBPase (65.8%) Sus scrofa

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.1.3.11 kidney
-
 Sus scrofa
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.3.11 D-fructose 1,6-bisphosphate + H2O
-
 Sus scrofa D-fructose 6-phosphate + phosphate
-
 ?

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.1.3.11 14.2
-
 D-fructose 1,6-bisphosphate 30°C, pH 7.5, mutant enzyme F232W Sus scrofa
3.1.3.11 18.1
-
 D-fructose 1,6-bisphosphate 30°C, pH 7.5, mutant enzyme F16W Sus scrofa
3.1.3.11 18.7
-
 D-fructose 1,6-bisphosphate 30°C, pH 7.5, mutant enzyme F219W Sus scrofa
3.1.3.11 19.2
-
 D-fructose 1,6-bisphosphate 30°C, pH 7.5, mutant enzyme F89W Sus scrofa
3.1.3.11 19.7
-
 D-fructose 1,6-bisphosphate 30°C, pH 7.5, recombinant wild-type enzyme Sus scrofa
3.1.3.11 20.7
-
 D-fructose 1,6-bisphosphate 30°C, pH 7.5, nonrecombinant enzyme Sus scrofa

IC50 Value

EC Number IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
3.1.3.11 0.0007
-
 30°C, pH 7.5, mutant enzyme F16W Sus scrofa D-fructose 2,6-bisphosphate
3.1.3.11 0.0007
-
 30°C, pH 7.5, mutant enzyme F89W Sus scrofa D-fructose 2,6-bisphosphate
3.1.3.11 0.0009
-
 30°C, pH 7.5, nonrecombinant enzyme Sus scrofa D-fructose 2,6-bisphosphate
3.1.3.11 0.001
-
 30°C, pH 7.5, recombinant wild-type enzyme Sus scrofa D-fructose 2,6-bisphosphate
3.1.3.11 0.0016
-
 30°C, pH 7.5, mutant enzyme F219W Sus scrofa D-fructose 2,6-bisphosphate
3.1.3.11 0.0019
-
 30°C, pH 7.5, mutant enzyme F232W Sus scrofa D-fructose 2,6-bisphosphate
3.1.3.11 0.0031
-
 30°C, pH 7.5, mutant enzyme F89W Sus scrofa AMP
3.1.3.11 0.0034
-
 30°C, pH 7.5, mutant enzyme F219W Sus scrofa AMP
3.1.3.11 0.0046
-
 30°C, pH 7.5, mutant enzyme F232W Sus scrofa AMP
3.1.3.11 0.0057
-
 30°C, pH 7.5, mutant enzyme F16W Sus scrofa AMP
3.1.3.11 0.0071
-
 30°C, pH 7.5, recombinant wild-type enzyme Sus scrofa AMP
3.1.3.11 0.0102
-
 30°C, pH 7.5, nonrecombinant enzyme Sus scrofa AMP