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Literature summary extracted from

  • Versees, W.; Spaepen, S.; Vanderleyden, J.; Steyaert, J.
    The crystal structure of phenylpyruvate decarboxylase from Azospirillum brasilense at 1.5 A resolution. Implications for its catalytic and regulatory mechanism (2007), FEBS J., 274, 2363-2375.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
4.1.1.43 expressed in Escherichia coli Bl21-CodonPlus (DE3)-RP cells Azospirillum brasilense

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
4.1.1.43 hanging drop vapour diffusion method with precipitant solution containing 15% PEG4000 (w /v), 10% glycerol (v /v) in 100 mM HEPES buffer pH 7.5, at 20°C Azospirillum brasilense

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
4.1.1.43 Mg2+ required, PPDC binds two molecules of Mg2+ per dimer Azospirillum brasilense

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
4.1.1.43 58000
-
 4 * 58000, X-ray crystallography, asymmetric dimer of dimers, in the asymmetric unit two subunits of PPDC are tightly packed to form a dimer related by noncrystallographic two-fold symmetry Azospirillum brasilense

Organism

EC Number Organism UniProt Comment Textmining
4.1.1.43 Azospirillum brasilense
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
4.1.1.43 Ni-nitrilotriacetic acid affinity chromatography and Superdex-200 gel filtration Azospirillum brasilense

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.1.1.43 indolepyruvate higher affinity for indolepyruvate than for phenylpyruvate but a 10fold higher specificity for the latter Azospirillum brasilense indoleacetaldehyde + CO2
-
 ?
4.1.1.43 additional information completely inactive toward benzoylformate Azospirillum brasilense ?
-
 ?
4.1.1.43 Phenylpyruvate converts phenylpyruvate with a 10fold higher specificity than indolepyruvate despite a slightly higher affinity for the latter Azospirillum brasilense Phenylacetaldehyde + CO2
-
 ?

Subunits

EC Number Subunits Comment Organism
4.1.1.43 homotetramer 4 * 58000, X-ray crystallography, asymmetric dimer of dimers, in the asymmetric unit two subunits of PPDC are tightly packed to form a dimer related by noncrystallographic two-fold symmetry Azospirillum brasilense

Synonyms

EC Number Synonyms Comment Organism
4.1.1.43 phenyl/indolepyruvate decarboxylase
-
 Azospirillum brasilense
4.1.1.43 PPDC
-
 Azospirillum brasilense

Cofactor

EC Number Cofactor Comment Organism Structure
4.1.1.43 thiamine diphosphate dependent, PPDC binds two molecules of thiamine diphosphate per dimer Azospirillum brasilense