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Literature summary extracted from

  • Reis, F.C.; Costa, T.F.; Sulea, T.; Mezzetti, A.; Scharfstein, J.; Broemme, D.; Menard, R.; Lima, A.P.
    The propeptide of cruzipain--a potent selective inhibitor of the trypanosomal enzymes cruzipain and brucipain, and of the human enzyme cathepsin F (2007), FEBS J., 274, 1224-1234.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.4.22.51 full-length propeptide of cruzipain is cloned by PCR and expressed in Escherichia coli as a fusion protein with a 6xHis tag at the N-terminus Trypanosoma cruzi

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.4.22.51 propeptide of cruzipain PCZ, is also a good inhibitor of brucipain from Trypanosoma brucei. The propeptide of cruzipain does not inhibit human cathepsins S, K or B or papain at the tested concentrations, and moderately inhibits human cathepsin V. Human cathepsin F is very efficiently inhibited (Ki of 32 picomol/L) Trypanosoma cruzi

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.4.22.51 14020
-
 MALDI-TOF Trypanosoma cruzi

Organism

EC Number Organism UniProt Comment Textmining
3.4.22.51 Trypanosoma cruzi
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.4.22.51 using an Ni-nitrilotriacetic acid-agarose resin Trypanosoma cruzi

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.4.22.51 Z-Phe-Arg-7-amido-4-methylcoumarin + H2O
-
 Trypanosoma cruzi Z-Phe-Arg + 7-amino-4-methylcoumarin
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.4.22.51 cruzipain
-
 Trypanosoma cruzi

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.4.22.51 6.5
-
 complete inhibition by the propeptide of cruzipain Trypanosoma cruzi

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
3.4.22.51 0.018
-
 propeptide of cruzipain at pH 6.5 the propeptide of cruzipain inactivates cruzipain competitively Trypanosoma cruzi
3.4.22.51 0.027
-
 propeptide of cruzipain at pH 6.0 Trypanosoma cruzi
3.4.22.51 0.034
-
 propeptide of cruzipain at pH 7.0 Trypanosoma cruzi
3.4.22.51 0.0565
-
 propeptide of cruzipain at pH 5.5 Trypanosoma cruzi
3.4.22.51 0.2637
-
 propeptide of cruzipain at pH5.0 Trypanosoma cruzi