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Literature summary extracted from

  • Jorge, C.D.; Sampaio, M.M.; Hreggvidsson, G.O.; Kristjanson, J.K.; Santos, H.
    A highly thermostable trehalase from the thermophilic bacterium Rhodothermus marinus (2007), Extremophiles, 11, 115-122.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.2.1.28 expressed in Escherichia coli DH5alpha cells Rhodothermus marinus

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.2.1.28 Co2+ 71% inhibition at 5 mM Rhodothermus marinus
3.2.1.28 D-glucose competitive inhibitor Rhodothermus marinus
3.2.1.28 EDTA moderate inhibitory level at 5 mM Rhodothermus marinus
3.2.1.28 Mg2+ moderate inhibitory level at 5 mM Rhodothermus marinus
3.2.1.28 Mn2+ moderate inhibitory level at 5 mM Rhodothermus marinus
3.2.1.28 additional information Ca2+ has no significant inhibitory effect at 5 mM Rhodothermus marinus
3.2.1.28 Zn2+ 95% inhibition at 5 mM Rhodothermus marinus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.2.1.28 0.16
-
 trehalose at 65°C, in 50 mM Bis/Tris/propane-HCl buffer (pH 6.5) Rhodothermus marinus
3.2.1.28 0.27
-
 trehalose at 88°C, in 50 mM Bis/Tris/propane-HCl buffer (pH 6.5) Rhodothermus marinus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.2.1.28 periplasm
-
 Rhodothermus marinus
-
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.1.28 59000
-
 SDS-PAGE Rhodothermus marinus
3.2.1.28 59270
-
 calculated from amino acid sequence Rhodothermus marinus
3.2.1.28 77000
-
 gel filtration Rhodothermus marinus

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.28 Rhodothermus marinus A1XBT9
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.28 ammonium sulfate precipitation, DEAE-Sepharose column chromatography, and Q Sepharose column chromatography Rhodothermus marinus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.28 trehalose + H2O high specificity for trehalose Rhodothermus marinus D-glucose
-
 ?

Subunits

EC Number Subunits Comment Organism
3.2.1.28 monomer 1 * 59000, SDS-PAGE Rhodothermus marinus

Synonyms

EC Number Synonyms Comment Organism
3.2.1.28 Alpha,alpha-trehalose glucohydrolase
-
 Rhodothermus marinus
3.2.1.28 trehalase
-
 Rhodothermus marinus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.28 88
-
-
 Rhodothermus marinus

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.2.1.28 70 90 the enzyme exhibits approximately a half-life of 6 h at 70°C, and of 2.5 h at 80°C, incubation of 10 min at 87°C results in 70% loss of the enzyme activity, and after 10 min at 90°C no residual activity is detected Rhodothermus marinus
3.2.1.28 80
-
 the recombinant trehalase with a histidine tag at the N-terminus loses 50% of the activity after 10 min incubation at 80°C in 50 mM Bis/Tris/propane-HCl buffer (pH 6.5) Rhodothermus marinus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.28 6.5
-
-
 Rhodothermus marinus

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
3.2.1.28 7
-
 D-glucose at 65°C, in 50 mM Bis/Tris/propane-HCl buffer (pH 6.5) Rhodothermus marinus