BRENDA - Enzyme Database

Purification and characterisation of an archaebacterial succinate dehydrogenase complex from the plasma membrane of the thermoacidophile Sulfolobus acidocaldarius.

Moll, R.; Schäfer, G.; Eur. J. Biochem. 201, 593-600 (1991)

Data extracted from this reference:

Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
1.3.5.1
malonate
-
Sulfolobus acidocaldarius
1.3.5.1
oxaloacetate
-
Sulfolobus acidocaldarius
1.3.5.1
tetrachlorobenzoquinone
strong inhibition of succinate-phenazine methosulfate-(1,4-dichloroindophenol) oxidoreductase
Sulfolobus acidocaldarius
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
1.3.5.1
0.0654
-
oxidized 2,6-dichlorophenolindophenol
pH 6.5, 55°C
Sulfolobus acidocaldarius
1.3.5.1
0.0991
-
oxidized N,N,N',N'-tetramethyl-4-phenylenediamine
pH 6.5, 55°C
Sulfolobus acidocaldarius
1.3.5.1
0.18
-
caldariellaquinone
pH 6.5, 55°C
Sulfolobus acidocaldarius
1.3.5.1
0.295
-
oxidized phenazine methosulfate
pH 6.5, 55°C
Sulfolobus acidocaldarius
1.3.5.1
1.14
-
ferrocyanide
pH 6.5, 55°C
Sulfolobus acidocaldarius
1.3.5.1
1.42
-
succinate
pH 6.5, 55°C
Sulfolobus acidocaldarius
Localization
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
1.3.5.1
plasma membrane
-
Sulfolobus acidocaldarius
5886
-
Metals/Ions
EC Number
Metals/Ions
Commentary
Organism
Structure
1.3.5.1
Iron
the enzyme complex contains 102.4 nmol/mg iron
Sulfolobus acidocaldarius
Molecular Weight [Da]
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
1.3.5.1
12800
-
x * 66000 (subunit a) + x * 31000 (subunit b) + x * 28000 (subunit c) + x * 12800 (subunit d), the four subunits are present in an equimolar stoichiometry, SDS-PAGE
Sulfolobus acidocaldarius
1.3.5.1
28000
-
x * 66000 (subunit a) + x * 31000 (subunit b) + x * 28000 (subunit c) + x * 12800 (subunit d), the four subunits are present in an equimolar stoichiometry, SDS-PAGE
Sulfolobus acidocaldarius
1.3.5.1
31000
-
x * 66000 (subunit a) + x * 31000 (subunit b) + x * 28000 (subunit c) + x * 12800 (subunit d), the four subunits are present in an equimolar stoichiometry, SDS-PAGE
Sulfolobus acidocaldarius
1.3.5.1
66000
-
x * 66000 (subunit a) + x * 31000 (subunit b) + x * 28000 (subunit c) + x * 12800 (subunit d), the four subunits are present in an equimolar stoichiometry, SDS-PAGE
Sulfolobus acidocaldarius
1.3.5.1
141000
-
gel filtration
Sulfolobus acidocaldarius
Natural Substrates/ Products (Substrates)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
1.3.5.1
succinate + caldariellaquinone
Sulfolobus acidocaldarius
-
fumarate + caldariellaquinol
-
-
?
1.3.5.1
succinate + caldariellaquinone
Sulfolobus acidocaldarius DSM 639
-
fumarate + caldariellaquinol
-
-
?
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
1.3.5.1
Sulfolobus acidocaldarius
P77943 and P77944 and P77945 and P77946
P77943: subunit sdhA (flavoprotein subunit), P77944: subunit sdhB, P77945: subunit sdhC, P77946: subunit sdhD
-
1.3.5.1
Sulfolobus acidocaldarius DSM 639
P77943 and P77944 and P77945 and P77946
P77943: subunit sdhA (flavoprotein subunit), P77944: subunit sdhB, P77945: subunit sdhC, P77946: subunit sdhD
-
Purification (Commentary)
EC Number
Commentary
Organism
1.3.5.1
-
Sulfolobus acidocaldarius
Source Tissue
EC Number
Source Tissue
Commentary
Organism
Textmining
Specific Activity [micromol/min/mg]
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
1.3.5.1
7.5
-
pH 6.5, 55°C
Sulfolobus acidocaldarius
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1.3.5.1
succinate + caldariellaquinone
-
679618
Sulfolobus acidocaldarius
fumarate + caldariellaquinol
-
-
-
?
1.3.5.1
succinate + caldariellaquinone
caldariellaquinone, the physiologically acting electron mediator in Sulfolobus membranes is slowly reduced as compared to water-soluble dyes
679618
Sulfolobus acidocaldarius
fumarate + caldariellaquinol
-
-
-
?
1.3.5.1
succinate + caldariellaquinone
-
679618
Sulfolobus acidocaldarius DSM 639
fumarate + caldariellaquinol
-
-
-
?
1.3.5.1
succinate + caldariellaquinone
caldariellaquinone, the physiologically acting electron mediator in Sulfolobus membranes is slowly reduced as compared to water-soluble dyes
679618
Sulfolobus acidocaldarius DSM 639
fumarate + caldariellaquinol
-
-
-
?
1.3.5.1
succinate + ferrocyanide
-
679618
Sulfolobus acidocaldarius
fumarate + ferricyanide
-
-
-
?
1.3.5.1
succinate + ferrocyanide
-
679618
Sulfolobus acidocaldarius DSM 639
fumarate + ferricyanide
-
-
-
?
1.3.5.1
succinate + oxidized 2,6-dichlorophenolindophenol
-
679618
Sulfolobus acidocaldarius
fumarate + reduced 2,6-dichlorophenolindophenol
-
-
-
?
1.3.5.1
succinate + oxidized 2,6-dichlorophenolindophenol
-
679618
Sulfolobus acidocaldarius DSM 639
fumarate + reduced 2,6-dichlorophenolindophenol
-
-
-
?
1.3.5.1
succinate + oxidized N,N,N',N'-tetramethyl-4-phenylenediamine
-
679618
Sulfolobus acidocaldarius
fumarate + reduced N,N,N',N'-tetramethyl-4-phenylenediamine
-
-
-
?
1.3.5.1
succinate + oxidized N,N,N',N'-tetramethyl-4-phenylenediamine
-
679618
Sulfolobus acidocaldarius DSM 639
fumarate + reduced N,N,N',N'-tetramethyl-4-phenylenediamine
-
-
-
?
1.3.5.1
succinate + oxidized phenazine methosulfate
-
679618
Sulfolobus acidocaldarius
fumarate + reduced phenazine methosulfate
-
-
-
?
Subunits
EC Number
Subunits
Commentary
Organism
1.3.5.1
tetramer
x * 66000 (subunit a) + x * 31000 (subunit b) + x * 28000 (subunit c) + x * 12800 (subunit d), the four subunits are present in an equimolar stoichiometry, SDS-PAGE
Sulfolobus acidocaldarius
Temperature Optimum [°C]
EC Number
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
1.3.5.1
81
-
-
Sulfolobus acidocaldarius
Temperature Range [°C]
EC Number
Temperature Minimum [°C]
Temperature Maximum [°C]
Commentary
Organism
1.3.5.1
60
87
60°C: about 40% of maximal activity, 87°C: about 55% of maximal activity
Sulfolobus acidocaldarius
Temperature Stability [°C]
EC Number
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
1.3.5.1
55
-
1 h, stable
Sulfolobus acidocaldarius
pH Optimum
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
1.3.5.1
6.5
-
-
Sulfolobus acidocaldarius
pH Range
EC Number
pH Minimum
pH Maximum
Commentary
Organism
1.3.5.1
5.5
7.5
pH 5.5: about 40% of maximal activity, pH 7.5: about 55% of maximal activity
Sulfolobus acidocaldarius
Cofactor
EC Number
Cofactor
Commentary
Organism
Structure
1.3.5.1
flavin
the enzyme complex contains non-extractable flavin. The purified complex contains 4.6 nmol/mg acid non-extractable flavin
Sulfolobus acidocaldarius
Cofactor (protein specific)
EC Number
Cofactor
Commentary
Organism
Structure
1.3.5.1
flavin
the enzyme complex contains non-extractable flavin. The purified complex contains 4.6 nmol/mg acid non-extractable flavin
Sulfolobus acidocaldarius
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
1.3.5.1
malonate
-
Sulfolobus acidocaldarius
1.3.5.1
oxaloacetate
-
Sulfolobus acidocaldarius
1.3.5.1
tetrachlorobenzoquinone
strong inhibition of succinate-phenazine methosulfate-(1,4-dichloroindophenol) oxidoreductase
Sulfolobus acidocaldarius
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
1.3.5.1
0.0654
-
oxidized 2,6-dichlorophenolindophenol
pH 6.5, 55°C
Sulfolobus acidocaldarius
1.3.5.1
0.0991
-
oxidized N,N,N',N'-tetramethyl-4-phenylenediamine
pH 6.5, 55°C
Sulfolobus acidocaldarius
1.3.5.1
0.18
-
caldariellaquinone
pH 6.5, 55°C
Sulfolobus acidocaldarius
1.3.5.1
0.295
-
oxidized phenazine methosulfate
pH 6.5, 55°C
Sulfolobus acidocaldarius
1.3.5.1
1.14
-
ferrocyanide
pH 6.5, 55°C
Sulfolobus acidocaldarius
1.3.5.1
1.42
-
succinate
pH 6.5, 55°C
Sulfolobus acidocaldarius
Localization (protein specific)
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
1.3.5.1
plasma membrane
-
Sulfolobus acidocaldarius
5886
-
Metals/Ions (protein specific)
EC Number
Metals/Ions
Commentary
Organism
Structure
1.3.5.1
Iron
the enzyme complex contains 102.4 nmol/mg iron
Sulfolobus acidocaldarius
Molecular Weight [Da] (protein specific)
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
1.3.5.1
12800
-
x * 66000 (subunit a) + x * 31000 (subunit b) + x * 28000 (subunit c) + x * 12800 (subunit d), the four subunits are present in an equimolar stoichiometry, SDS-PAGE
Sulfolobus acidocaldarius
1.3.5.1
28000
-
x * 66000 (subunit a) + x * 31000 (subunit b) + x * 28000 (subunit c) + x * 12800 (subunit d), the four subunits are present in an equimolar stoichiometry, SDS-PAGE
Sulfolobus acidocaldarius
1.3.5.1
31000
-
x * 66000 (subunit a) + x * 31000 (subunit b) + x * 28000 (subunit c) + x * 12800 (subunit d), the four subunits are present in an equimolar stoichiometry, SDS-PAGE
Sulfolobus acidocaldarius
1.3.5.1
66000
-
x * 66000 (subunit a) + x * 31000 (subunit b) + x * 28000 (subunit c) + x * 12800 (subunit d), the four subunits are present in an equimolar stoichiometry, SDS-PAGE
Sulfolobus acidocaldarius
1.3.5.1
141000
-
gel filtration
Sulfolobus acidocaldarius
Natural Substrates/ Products (Substrates) (protein specific)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
1.3.5.1
succinate + caldariellaquinone
Sulfolobus acidocaldarius
-
fumarate + caldariellaquinol
-
-
?
1.3.5.1
succinate + caldariellaquinone
Sulfolobus acidocaldarius DSM 639
-
fumarate + caldariellaquinol
-
-
?
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
1.3.5.1
-
Sulfolobus acidocaldarius
Source Tissue (protein specific)
EC Number
Source Tissue
Commentary
Organism
Textmining
Specific Activity [micromol/min/mg] (protein specific)
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
1.3.5.1
7.5
-
pH 6.5, 55°C
Sulfolobus acidocaldarius
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1.3.5.1
succinate + caldariellaquinone
-
679618
Sulfolobus acidocaldarius
fumarate + caldariellaquinol
-
-
-
?
1.3.5.1
succinate + caldariellaquinone
caldariellaquinone, the physiologically acting electron mediator in Sulfolobus membranes is slowly reduced as compared to water-soluble dyes
679618
Sulfolobus acidocaldarius
fumarate + caldariellaquinol
-
-
-
?
1.3.5.1
succinate + caldariellaquinone
-
679618
Sulfolobus acidocaldarius DSM 639
fumarate + caldariellaquinol
-
-
-
?
1.3.5.1
succinate + caldariellaquinone
caldariellaquinone, the physiologically acting electron mediator in Sulfolobus membranes is slowly reduced as compared to water-soluble dyes
679618
Sulfolobus acidocaldarius DSM 639
fumarate + caldariellaquinol
-
-
-
?
1.3.5.1
succinate + ferrocyanide
-
679618
Sulfolobus acidocaldarius
fumarate + ferricyanide
-
-
-
?
1.3.5.1
succinate + ferrocyanide
-
679618
Sulfolobus acidocaldarius DSM 639
fumarate + ferricyanide
-
-
-
?
1.3.5.1
succinate + oxidized 2,6-dichlorophenolindophenol
-
679618
Sulfolobus acidocaldarius
fumarate + reduced 2,6-dichlorophenolindophenol
-
-
-
?
1.3.5.1
succinate + oxidized 2,6-dichlorophenolindophenol
-
679618
Sulfolobus acidocaldarius DSM 639
fumarate + reduced 2,6-dichlorophenolindophenol
-
-
-
?
1.3.5.1
succinate + oxidized N,N,N',N'-tetramethyl-4-phenylenediamine
-
679618
Sulfolobus acidocaldarius
fumarate + reduced N,N,N',N'-tetramethyl-4-phenylenediamine
-
-
-
?
1.3.5.1
succinate + oxidized N,N,N',N'-tetramethyl-4-phenylenediamine
-
679618
Sulfolobus acidocaldarius DSM 639
fumarate + reduced N,N,N',N'-tetramethyl-4-phenylenediamine
-
-
-
?
1.3.5.1
succinate + oxidized phenazine methosulfate
-
679618
Sulfolobus acidocaldarius
fumarate + reduced phenazine methosulfate
-
-
-
?
Subunits (protein specific)
EC Number
Subunits
Commentary
Organism
1.3.5.1
tetramer
x * 66000 (subunit a) + x * 31000 (subunit b) + x * 28000 (subunit c) + x * 12800 (subunit d), the four subunits are present in an equimolar stoichiometry, SDS-PAGE
Sulfolobus acidocaldarius
Temperature Optimum [°C] (protein specific)
EC Number
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
1.3.5.1
81
-
-
Sulfolobus acidocaldarius
Temperature Range [°C] (protein specific)
EC Number
Temperature Minimum [°C]
Temperature Maximum [°C]
Commentary
Organism
1.3.5.1
60
87
60°C: about 40% of maximal activity, 87°C: about 55% of maximal activity
Sulfolobus acidocaldarius
Temperature Stability [°C] (protein specific)
EC Number
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
1.3.5.1
55
-
1 h, stable
Sulfolobus acidocaldarius
pH Optimum (protein specific)
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
1.3.5.1
6.5
-
-
Sulfolobus acidocaldarius
pH Range (protein specific)
EC Number
pH Minimum
pH Maximum
Commentary
Organism
1.3.5.1
5.5
7.5
pH 5.5: about 40% of maximal activity, pH 7.5: about 55% of maximal activity
Sulfolobus acidocaldarius