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Literature summary extracted from

  • Matvienko, N.I.; Kramarov, V.M.; Pachkunov, D.M.
    Isolation and some properties of the site-specific endonuclease and methylase Bme2161 from Bacillus megaterium 216 (1987), Eur. J. Biochem., 165, 565-570.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.1.21.4 Co2+ complete inhibition at 1 mM Priestia megaterium
3.1.21.4 Mn2+ complete inhibition at 3 mM Priestia megaterium
3.1.21.4 NaCl inhibitory above 0.2 mM Priestia megaterium
3.1.21.4 Ni2+ complete inhibition at 1 mM Priestia megaterium
3.1.21.4 Zn2+
-
 Priestia megaterium

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.1.21.4 Co2+ can replace Mn2+ but yields a much lower activity than Mg2+, highest activity at 0.1 mM, inhibitory at high concentrations Priestia megaterium
3.1.21.4 Mg2+ highest activity at 5-7 mM Priestia megaterium
3.1.21.4 Mn2+ can replace Mn2+ but yields a much lower activity than Mg2+, highest activity at 0.3 mM, inhibitory at high concentrations Priestia megaterium
3.1.21.4 Ni2+ can replace Mn2+ but yields a much lower activity than Mg2+, highest activity at 0.1 mM, inhibitory at high concentrations Priestia megaterium

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.1.21.4 30000
-
 x * 30000, SDS-PAGE, native mass by gel filtration Priestia megaterium
3.1.21.4 60000
-
 gel filtration Priestia megaterium

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.1.21.4 dsDNA + H2O Priestia megaterium recognition sequence is 5’-GGACC-3’/3’-CCTGG-5’, enzyme cleaves between the guanosin residues at both strands ? sticky end fragments ?
3.1.21.4 dsDNA + H2O Priestia megaterium 216 recognition sequence is 5’-GGACC-3’/3’-CCTGG-5’, enzyme cleaves between the guanosin residues at both strands ? sticky end fragments ?

Organism

EC Number Organism UniProt Comment Textmining
3.1.21.4 Priestia megaterium
-
-
-
3.1.21.4 Priestia megaterium 216
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.1.21.4
-
 Priestia megaterium

Storage Stability

EC Number Storage Stability Organism
3.1.21.4 -20°C, 10 mM phosphate buffer, pH 7.4, 0.1 mM EDTA, 2 mM dithiothreitol, 0.2 M NaCl, 50% glycerol, fully stable for at least 1 year Priestia megaterium

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.21.4 dsDNA + H2O recognition sequence is 5’-GGACC-3’/3’-CCTGG-5’, enzyme cleaves between the guanosin residues at both strands Priestia megaterium ? sticky end fragments ?
3.1.21.4 dsDNA + H2O recognition sequence is 5’-GGACC-3’/3’-CCTGG-5’, enzyme cleaves between the guanosin residues at both strands Priestia megaterium 216 ? sticky end fragments ?
3.1.21.4 pBR322 DNA + H2O
-
 Priestia megaterium ?
-
 ?
3.1.21.4 pBR322 DNA + H2O
-
 Priestia megaterium 216 ?
-
 ?

Subunits

EC Number Subunits Comment Organism
3.1.21.4 dimer x * 30000, SDS-PAGE, native mass by gel filtration Priestia megaterium

Synonyms

EC Number Synonyms Comment Organism
3.1.21.4 site-specific endonuclease Bme216I recognition site is identical with the endonuclease AvaII from Anabaena variabilis Priestia megaterium

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.1.21.4 6 9
-
 Priestia megaterium