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Literature summary extracted from

  • Mendoza-Palomares, C.; Biteau, N.; Giroud, C.; Coustou, V.; Coetzer, T.; Authie, E.; Boulange, A.; Baltz, T.
    Molecular and biochemical characterization of a cathepsin B-like protease family unique to Trypanosoma congolense (2008), Eukaryot. Cell, 7, 684-697.
    View publication on PubMedView publication on EuropePMC

Activating Compound

EC Number Activating Compound Comment Organism Structure
3.4.22.B6 DTT no activity in buffer lacking DTT Trypanosoma congolense

Application

EC Number Application Comment Organism
3.4.22.B6 medicine TcoCBc1 elicits an important immune response in experimentally infected cattle. The protein is a potential therapeutic target and is a plausible antigen for Trypanosoma congolense diagnosis Trypanosoma congolense

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.4.22.B6 six-His-tagged protein is expressed in Pichia pastoris strain X-33 Trypanosoma congolense

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.4.22.B6 acetyl-Leu-Val-lysinal 350 nM, 37°C, pH 4.5, 1 h, 15% inhibition Trypanosoma congolense
3.4.22.B6 CA074 350 nM, 37°C, pH 4.5, 1 h, complete inhibition Trypanosoma congolense
3.4.22.B6 CBZ-Phe-Gly-NHO-Bz-pMe 350 nM, 37°X, pH 4.5, 1 h, 87% inhibition Trypanosoma congolense
3.4.22.B6 cystatin 350 nM, 37°C, pH 4.5, 1 h, 23% inhibition Trypanosoma congolense
3.4.22.B6 E-64 350 nM, 37°C, pH 4.5, 1 h, complete inhibition Trypanosoma congolense

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.4.22.B6 0.1234
-
 Nalpha-benzyloxycarbonyl-Phe-Arg-7-amido-4-methylcoumarin
-
 Trypanosoma congolense
3.4.22.B6 0.1733
-
 N2-benzyloxycarbonyl-Arg-Arg-7-amido-4-methylcoumarin
-
 Trypanosoma congolense

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.4.22.B6 lysosome
-
 Trypanosoma congolense 5764
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.4.22.B6 additional information
-
 SDS-PAGE analysis of the eluted recombinant protein reveals a prominent band of 39000 Da and a minor protein of 40000 Da. These bands correspond to glycosylated forms of a 35000 Da protein, the expected size of the protease Trypanosoma congolense
3.4.22.B6 additional information
-
 SDS-PAGE analysis of the eluted recombinant protein reveals a prominent band of 40000 Da and a minor protein of 50000 Da. These bands correspond to glycosylated forms of a 36000 Da protein, the expected size of the protease Trypanosoma congolense

Organism

EC Number Organism UniProt Comment Textmining
3.4.22.B6 Trypanosoma congolense
-
-
-
3.4.22.B6 Trypanosoma congolense B2C323
-
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
3.4.22.B6 proteolytic modification TcoCBc1 is able to autocatalyze its maturation 21 residues upstream of the predicted start of the catalytic domain Trypanosoma congolense
3.4.22.B6 proteolytic modification TcoCBc6 is able to autocatalyze its maturation 31 residues, upstream of the predicted start of the catalytic domain Trypanosoma congolense

Purification (Commentary)

EC Number Purification (Comment) Organism
3.4.22.B6
-
 Trypanosoma congolense

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.4.22.B6 bloodstream form
-
 Trypanosoma congolense
-
3.4.22.B6 bloodstream form expressed mainly in bloodstream form Trypanosoma congolense
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.4.22.B6 2-aminobenzoyl-Phe-Arg-Ala-(2,4-dinitrophenyl)-epsilon-NH2-lysine + H2O
-
 Trypanosoma congolense ?
-
 ?
3.4.22.B6 benzyloxycarbonyl-Arg-Arg-7-amido-4-methylcoumarin + H2O
-
 Trypanosoma congolense CBZ-Arg-Arg + 7-amino-4-methylcoumarin
-
 ?
3.4.22.B6 benzyloxycarbonyl-Phe-Arg-7-amido-4-methylcoumarin + H2O
-
 Trypanosoma congolense benzyloxycarbonyl-Phe-Arg + 7-amino-4-methylcoumarin
-
 ?
3.4.22.B6 additional information no hydrolysis of benzoyloxycarbonyl-Phe-Arg-7-amido-4-methylcoumarin and benzoyloxycarbonyl-Arg-Arg-7-amido-4-methylcoumarin Trypanosoma congolense ?
-
 ?
3.4.22.B6 Nalpha-benzyloxycarbonyl-Phe-Arg-7-amido-4-methylcoumarin + H2O
-
 Trypanosoma congolense Nalpha-benzyloxycarbonyl-Phe-Arg + 7-amino-4-methylcoumarin
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.4.22.B6 CBc1
-
 Trypanosoma congolense
3.4.22.B6 CBc6
-
 Trypanosoma congolense
3.4.22.B6 TcoCBc1
-
 Trypanosoma congolense
3.4.22.B6 TcoCBc6
-
 Trypanosoma congolense

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.4.22.B6 1.09
-
 Nalpha-benzyloxycarbonyl-Phe-Arg-7-amido-4-methylcoumarin
-
 Trypanosoma congolense
3.4.22.B6 3 6 CBZ-Arg-Arg-7-amido-4-methylcoumarin
-
 Trypanosoma congolense
3.4.22.B6 3.49
-
 N2-benzyloxycarbonyl-Arg-Arg-7-amido-4-methylcoumarin
-
 Trypanosoma congolense

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.4.22.B6 4.5
-
-
 Trypanosoma congolense

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.4.22.B6 4 6.5 pH 4.0: about 30% of maximal activity, pH 6.5: about 75% of maximal activity Trypanosoma congolense