EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
3.2.1.22 | Ag+ | no activity at 1 mM | Rhizopus sp. | |
3.2.1.22 | Al3+ | 66.7% activity at 1 mM | Rhizopus sp. | |
3.2.1.22 | Ca2+ | 82.2% activity at 1 mM | Rhizopus sp. | |
3.2.1.22 | Co2+ | 96.6% activity at 1 mM | Rhizopus sp. | |
3.2.1.22 | Cr3+ | 63.9% activity at 1 mM | Rhizopus sp. | |
3.2.1.22 | Cu2+ | 78.1% activity at 1 mM | Rhizopus sp. | |
3.2.1.22 | EDTA | 98.1% activity at 1 mM | Rhizopus sp. | |
3.2.1.22 | Fe2+ | 75.1% activity at 1 mM | Rhizopus sp. | |
3.2.1.22 | Hg2+ | no activity at 1 mM | Rhizopus sp. | |
3.2.1.22 | Li+ | 96.7% activity at 1 mM | Rhizopus sp. | |
3.2.1.22 | Mg2+ | 98.2% activity at 1 mM | Rhizopus sp. | |
3.2.1.22 | Mn2+ | 49.5% activity at 1 mM | Rhizopus sp. | |
3.2.1.22 | additional information | not inhibited by Na+ and Zn2+ | Rhizopus sp. | |
3.2.1.22 | Ni(2+) | 92.7% activity at 1 mM | Rhizopus sp. | |
3.2.1.22 | SDS | no activity at 1 mM | Rhizopus sp. |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
3.2.1.22 | 2.9 | - |
p-nitrophenyl alpha-D-galactopyranoside | in 0.1 M McIlvaine buffer (pH 4.8) at 37°C | Rhizopus sp. | |
3.2.1.22 | 33 | - |
melibiose | in 0.1 M McIlvaine buffer (pH 4.8) at 37°C | Rhizopus sp. |
EC Number | Metals/Ions | Comment | Organism | Structure |
---|---|---|---|---|
3.2.1.22 | K+ | 9.5% increased activity at 1 mM | Rhizopus sp. |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
3.2.1.22 | 78000 | - |
SDS-PAGE | Rhizopus sp. |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
3.2.1.22 | Rhizopus sp. | - |
18S rRNA sequence; ACCC 30795 | - |
3.2.1.22 | Rhizopus sp. F78 | - |
18S rRNA sequence; ACCC 30795 | - |
EC Number | Purification (Comment) | Organism |
---|---|---|
3.2.1.22 | ammonium sulfate precipitation, HiTrap Q Sepharose XL column chromatography, and Sephacryl S-200 HR gel filtration | Rhizopus sp. |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
3.2.1.22 | 1.2 | - |
crude filtrate | Rhizopus sp. |
3.2.1.22 | 74.6 | - |
after 60.4fold purification | Rhizopus sp. |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
3.2.1.22 | melibiose + H2O | - |
Rhizopus sp. | D-galactose + D-glucose | - |
? | |
3.2.1.22 | melibiose + H2O | - |
Rhizopus sp. F78 | D-galactose + D-glucose | - |
? | |
3.2.1.22 | additional information | no detectable activity towards guar gum | Rhizopus sp. | ? | - |
? | |
3.2.1.22 | additional information | no detectable activity towards guar gum | Rhizopus sp. F78 | ? | - |
? | |
3.2.1.22 | p-nitrophenyl alpha-D-galactopyranoside + H2O | - |
Rhizopus sp. | p-nitrophenol + alpha-D-galactose | - |
? | |
3.2.1.22 | p-nitrophenyl alpha-D-galactopyranoside + H2O | - |
Rhizopus sp. F78 | p-nitrophenol + alpha-D-galactose | - |
? | |
3.2.1.22 | raffinose + H2O | - |
Rhizopus sp. | D-galactose + sucrose | - |
? | |
3.2.1.22 | raffinose + H2O | - |
Rhizopus sp. F78 | D-galactose + sucrose | - |
? | |
3.2.1.22 | stachyose + 2 H2O | - |
Rhizopus sp. | 2 alpha-D-galactose + sucrose | - |
? | |
3.2.1.22 | stachyose + 2 H2O | - |
Rhizopus sp. F78 | 2 alpha-D-galactose + sucrose | - |
? |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
3.2.1.22 | Aga-F78 | - |
Rhizopus sp. |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
3.2.1.22 | 50 | - |
- |
Rhizopus sp. |
EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|---|
3.2.1.22 | 40 | 60 | activity is 80% of maximum between 40°C and 55°C, the enzyme retains less than 25% of its activity at above 60°C and is stable at 50°C, no activity is lost after 30 min incubation, after 10, 20 and 30 min incubation at 60°C, 38%, 18% and 7% of activity remains, respectively | Rhizopus sp. |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
3.2.1.22 | 4.8 | - |
- |
Rhizopus sp. |
EC Number | pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|---|
3.2.1.22 | 3 | 10 | activity is not detected when the enzyme is reacted in buffers under pH 3.0 or above pH 10.0, activity remains above 80% between pH 4.6-5.2, the enzyme retains more than 80% of its activity between pH 5.0 and 10.0 | Rhizopus sp. |