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Literature summary extracted from

  • Benech, R.; Li, X.; Patton, D.; Powlowski, J.; Storms, R.; Bourbonnais, R.; Paice, M.; Tsang, A.
    Recombinant expression, characterization, and pulp prebleaching property of a Phanerochaete chrysosporium endo-beta -1,4-mannanase (2007), Enzyme Microb. Technol., 41, 740-747.
No PubMed abstract available

Application

EC Number Application Comment Organism
3.2.1.78 industry when used to treat softwood pulp, enzyme hydrolyzes mainly glucomannan and exhibits a positive effect as a prebleaching agent Phanerodontia chrysosporium

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.2.1.78 expression in Aspergillus niger Phanerodontia chrysosporium

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.2.1.78 additional information
-
 additional information apparent Km values of the mannanase for locust bean gum, glucomannan, glactomannan and mannan are 3, 2.3, 1.6 and 0.54 mg/ml, respectively Phanerodontia chrysosporium

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.2.1.78 extracellular
-
 Phanerodontia chrysosporium
-
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.1.78 65000
-
 x * 65000, SDS-PAGE Phanerodontia chrysosporium

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.78 Phanerodontia chrysosporium Q0PQY8
-
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
3.2.1.78 proteolytic modification sequence contains a secretory signal peptide Phanerodontia chrysosporium

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.2.1.78 culture fluid
-
 Phanerodontia chrysosporium
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.78 galactomannan + H2O
-
 Phanerodontia chrysosporium hydrolyzed galactomannan
-
 ?
3.2.1.78 glucomannan + H2O
-
 Phanerodontia chrysosporium oligosaccharides
-
 ?
3.2.1.78 locust bean gum + H2O
-
 Phanerodontia chrysosporium ?
-
 ?
3.2.1.78 mannan + H2O
-
 Phanerodontia chrysosporium oligosaccharides
-
 ?
3.2.1.78 additional information enzyme exhibits substantial transglycosylase activity Phanerodontia chrysosporium ?
-
 ?

Subunits

EC Number Subunits Comment Organism
3.2.1.78 ? x * 65000, SDS-PAGE Phanerodontia chrysosporium

Synonyms

EC Number Synonyms Comment Organism
3.2.1.78 man5D
-
 Phanerodontia chrysosporium

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.78 60
-
-
 Phanerodontia chrysosporium

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.2.1.78 60
-
 stable up to Phanerodontia chrysosporium

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.78 4 6 broad Phanerodontia chrysosporium

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
3.2.1.78 4 8
-
 Phanerodontia chrysosporium