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Cloning, characterization and expression analysis of alpha -glucuronidase from the thermophilic fungus Talaromyces emersonii

Heneghan, M.N.; McLoughlin, L.; Murray, P.G.; Tuohy, M.G.; Enzyme Microb. Technol. 41, 677-682 (2007)
No PubMed abstract available

Data extracted from this reference:

Activating Compound
EC Number
Activating Compound
Commentary
Organism
Structure
3.2.1.139
solka floc
low levels of activity
Rasamsonia emersonii
3.2.1.139
xylan
induces activity, maximum activity on beechwood xylan, activity also on oat spelt and birchwood xylan, low levels of activity on wheat arabinoxylan and larchwood xylan
Rasamsonia emersonii
Cloned(Commentary)
EC Number
Commentary
Organism
3.2.1.139
into pGEM-T Easy vector, plasmids maintained in Escherichia coli JM109
Rasamsonia emersonii
Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
3.2.1.139
D-glucose
represses
Rasamsonia emersonii
3.2.1.139
D-glucuronic acid
represses
Rasamsonia emersonii
Molecular Weight [Da]
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
3.2.1.139
91600
-
sequence analysis
Rasamsonia emersonii
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
3.2.1.139
Rasamsonia emersonii
-
-
-
3.2.1.139
Rasamsonia emersonii CBS 814.70
-
-
-
Posttranslational Modification
EC Number
Posttranslational Modification
Commentary
Organism
3.2.1.139
additional information
twenty putative N-glycosylation sites and four O-glycosylation sites
Rasamsonia emersonii
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
3.2.1.139
aldopentaouronic acid + H2O
-
679583
Rasamsonia emersonii
?
-
-
-
?
3.2.1.139
aldopentaouronic acid + H2O
-
679583
Rasamsonia emersonii CBS 814.70
?
-
-
-
?
3.2.1.139
aldotetraouronic acid + H2O
-
679583
Rasamsonia emersonii
?
-
-
-
?
3.2.1.139
aldotetraouronic acid + H2O
-
679583
Rasamsonia emersonii CBS 814.70
?
-
-
-
?
Temperature Optimum [°C]
EC Number
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
3.2.1.139
50
-
-
Rasamsonia emersonii
pH Optimum
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
3.2.1.139
5
-
-
Rasamsonia emersonii
pI Value
EC Number
Organism
Commentary
pI Value Maximum
pI Value
3.2.1.139
Rasamsonia emersonii
sequence analysis
-
6.1
Activating Compound (protein specific)
EC Number
Activating Compound
Commentary
Organism
Structure
3.2.1.139
solka floc
low levels of activity
Rasamsonia emersonii
3.2.1.139
xylan
induces activity, maximum activity on beechwood xylan, activity also on oat spelt and birchwood xylan, low levels of activity on wheat arabinoxylan and larchwood xylan
Rasamsonia emersonii
Cloned(Commentary) (protein specific)
EC Number
Commentary
Organism
3.2.1.139
into pGEM-T Easy vector, plasmids maintained in Escherichia coli JM109
Rasamsonia emersonii
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
3.2.1.139
D-glucose
represses
Rasamsonia emersonii
3.2.1.139
D-glucuronic acid
represses
Rasamsonia emersonii
Molecular Weight [Da] (protein specific)
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
3.2.1.139
91600
-
sequence analysis
Rasamsonia emersonii
Posttranslational Modification (protein specific)
EC Number
Posttranslational Modification
Commentary
Organism
3.2.1.139
additional information
twenty putative N-glycosylation sites and four O-glycosylation sites
Rasamsonia emersonii
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
3.2.1.139
aldopentaouronic acid + H2O
-
679583
Rasamsonia emersonii
?
-
-
-
?
3.2.1.139
aldopentaouronic acid + H2O
-
679583
Rasamsonia emersonii CBS 814.70
?
-
-
-
?
3.2.1.139
aldotetraouronic acid + H2O
-
679583
Rasamsonia emersonii
?
-
-
-
?
3.2.1.139
aldotetraouronic acid + H2O
-
679583
Rasamsonia emersonii CBS 814.70
?
-
-
-
?
Temperature Optimum [°C] (protein specific)
EC Number
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
3.2.1.139
50
-
-
Rasamsonia emersonii
pH Optimum (protein specific)
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
3.2.1.139
5
-
-
Rasamsonia emersonii
pI Value (protein specific)
EC Number
Organism
Commentary
pI Value Maximum
pI Value
3.2.1.139
Rasamsonia emersonii
sequence analysis
-
6.1