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Literature summary extracted from

  • Li, H.; Chi, Z.; Wang, X.; Duan, X.; Ma, L.; Gao, L.
    Purification and characterization of extracellular amylase from the marine yeast Aureobasidium pullulans N13d and its raw potato starch digestion (2007), Enzyme Microb. Technol., 40, 1006-1012.
No PubMed abstract available

General Stability

EC Number General Stability Organism
3.2.1.3 CaCl2 at 1 mM stabilizes the purified native enzyme Aureobasidium pullulans

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.2.1.3 Ag+
-
 Aureobasidium pullulans
3.2.1.3 EDTA
-
 Aureobasidium pullulans
3.2.1.3 EGTA
-
 Aureobasidium pullulans
3.2.1.3 Hg2+
-
 Aureobasidium pullulans
3.2.1.3 additional information no inhibition by iodoacetic acid and PMSF Aureobasidium pullulans
3.2.1.3 SDS
-
 Aureobasidium pullulans

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.2.1.3 5.75
-
 soluble starch pH 4.5, 60°C Aureobasidium pullulans

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.2.1.3 extracellular
-
 Aureobasidium pullulans
-
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.2.1.3 Ba2+ activates Aureobasidium pullulans
3.2.1.3 Ca2+ activates Aureobasidium pullulans
3.2.1.3 Co2+ activates Aureobasidium pullulans
3.2.1.3 Cu2+ activates Aureobasidium pullulans
3.2.1.3 Mg2+ activates Aureobasidium pullulans
3.2.1.3 Na+ activates Aureobasidium pullulans

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.1.3 33000
-
 1 * 65000 + 1 * 33000, SDS-PAGE Aureobasidium pullulans
3.2.1.3 65000
-
 1 * 65000 + 1 * 33000, SDS-PAGE Aureobasidium pullulans
3.2.1.3 98000
-
 native extracellular enzyme, native PAGE Aureobasidium pullulans

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.2.1.3 starch + H2O Aureobasidium pullulans
-
 D-glucose + ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.3 Aureobasidium pullulans
-
 marine yeast, strain N13d isolated from deep sea of Pacific Ocean
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.3 native extracellular enzyme from culture supernatant 7.3fold to homogeneity by ammonium sulfate fractionation and gel filtration Aureobasidium pullulans

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.2.1.3 cell culture
-
 Aureobasidium pullulans
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.2.1.3 297.3
-
 purified native enzyme Aureobasidium pullulans

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.3 additional information the enzyme has debranching activity Aureobasidium pullulans ?
-
 ?
3.2.1.3 soluble starch + H2O
-
 Aureobasidium pullulans beta-D-glucose + soluble starch
-
 ?
3.2.1.3 starch + H2O
-
 Aureobasidium pullulans D-glucose + ?
-
 ?
3.2.1.3 starch + H2O raw and cooked soluble starch from potato, no activity with corn starch and sweet potato starch Aureobasidium pullulans D-glucose + ?
-
 ?

Subunits

EC Number Subunits Comment Organism
3.2.1.3 dimer 1 * 65000 + 1 * 33000, SDS-PAGE Aureobasidium pullulans

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.3 60
-
-
 Aureobasidium pullulans

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.3 4.5
-
-
 Aureobasidium pullulans

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
3.2.1.3 3
-
 purified native enzyme, partial inactivation Aureobasidium pullulans
3.2.1.3 4 8 purified native enzyme, stable, over 75% remaining activity after 3 h at 55°C Aureobasidium pullulans
3.2.1.3 9
-
 purified native enzyme, partial inactivation Aureobasidium pullulans