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Literature summary extracted from

  • Hidaka, Y.; Hatada, Y.; Akita, M.; Yoshida, M.; Nakamura, N.; Takada, M.; Nakakuki, T.; Ito, S.; Horikoshi, K.
    Maltose phosphorylase from a deep-sea Paenibacillus sp.: Enzymatic properties and nucleotide and amino-acid sequences (2005), Enzyme Microb. Technol., 37, 185-194.
No PubMed abstract available

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.4.1.8 overexpressed in Escherichia coli Paenibacillus sp.

Protein Variants

EC Number Protein Variants Comment Organism
2.4.1.8 E487A mutant loses both the phosphorolytic and the synthetic activity Paenibacillus sp.
2.4.1.8 E487Q mutant loses the phosphorolytic activity, but retains the synthetic activity (13% of the activity of wild-type MapA) Paenibacillus sp.
2.4.1.8 S634V mutant loses both the phosphorolytic and the synthetic activity Paenibacillus sp.
2.4.1.8 S635V mutant loses both the phosphorolytic and the synthetic activity Paenibacillus sp.
2.4.1.8 Y351F mutant loses both the phosphorolytic and the synthetic activity Paenibacillus sp.

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.4.1.8 4-chloromercuribenzoate
-
 Paenibacillus sp.
2.4.1.8 Cu2+ 1 mM, 50°C, 30 min, abolishes the phosphorolytic activity almost completely Paenibacillus sp.
2.4.1.8 diethyldicarbonate
-
 Paenibacillus sp.
2.4.1.8 Hg2+ 1 mM, 50°C, 30 min, abolishes the phosphorolytic activity almost completely Paenibacillus sp.
2.4.1.8 additional information iodoacetoamide, EDTA, and phenylmethanesulfonyl fluoride have no inhibitory effect Paenibacillus sp.
2.4.1.8 N-bromosuccinimide 0.1 mM, 47% inhibition Paenibacillus sp.
2.4.1.8 N-ethylmaleimide
-
 Paenibacillus sp.

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.4.1.8 0.0036
-
 beta-D-glucose 1-phosphate 50°C, pH 7.0, mutant enzyme E487Q Paenibacillus sp.
2.4.1.8 0.004
-
 maltose 50°C, pH 7.0, wild-type enzyme Paenibacillus sp.
2.4.1.8 0.0043
-
 D-glucose 50°C, pH 7.0, mutant enzyme E487Q Paenibacillus sp.
2.4.1.8 0.0067
-
 beta-D-glucose 1-phosphate 50°C, pH 7.0, wild-type enzyme Paenibacillus sp.
2.4.1.8 0.0071
-
 phosphate 50°C, pH 7.0, wild-type enzyme Paenibacillus sp.
2.4.1.8 0.0099
-
 D-glucose 50°C, pH 7.0, wild-type enzyme Paenibacillus sp.

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.4.1.8 Fe3+ 1 mM, 50°C, 30 min, enhances the activity moderately Paenibacillus sp.
2.4.1.8 Mn2+ 1 mM, 50°C, 30 min, enhances the activity moderately Paenibacillus sp.
2.4.1.8 Pb2+ 1 mM, 50°C, 30 min, enhances the activity moderately Paenibacillus sp.
2.4.1.8 Sn2+ 1 mM, 50°C, 30 min, enhances the activity moderately Paenibacillus sp.

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.4.1.8 87748
-
 2 * 87748, calculated from sequence Paenibacillus sp.
2.4.1.8 90000
-
 2 * 90000, SDS-PAGE Paenibacillus sp.
2.4.1.8 180000
-
 gel filtration Paenibacillus sp.

Organism

EC Number Organism UniProt Comment Textmining
2.4.1.8 Paenibacillus sp. Q50LH0
-
-
2.4.1.8 Paenibacillus sp. SH-55 Q50LH0
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.4.1.8
-
 Paenibacillus sp.

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.4.1.8 50
-
-
 Paenibacillus sp.

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.4.1.8 maltose + phosphate
-
 Paenibacillus sp. D-glucose + beta-D-glucose 1-phosphate
-
 r
2.4.1.8 maltose + phosphate
-
 Paenibacillus sp. SH-55 D-glucose + beta-D-glucose 1-phosphate
-
 r
2.4.1.8 additional information no activity with: isomaltose, trehalose, neotrehalose, sucrose, lactose, and cellobiose Paenibacillus sp. ?
-
 ?
2.4.1.8 additional information no activity with: isomaltose, trehalose, neotrehalose, sucrose, lactose, and cellobiose Paenibacillus sp. SH-55 ?
-
 ?

Subunits

EC Number Subunits Comment Organism
2.4.1.8 dimer 2 * 90000, SDS-PAGE Paenibacillus sp.
2.4.1.8 dimer 2 * 87748, calculated from sequence Paenibacillus sp.

Synonyms

EC Number Synonyms Comment Organism
2.4.1.8 mpA
-
 Paenibacillus sp.

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.4.1.8 40
-
 glycosynthesis Paenibacillus sp.
2.4.1.8 50
-
 phosphorolysis Paenibacillus sp.

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
2.4.1.8 50
-
 15 min, pH 7.0, stable up to Paenibacillus sp.
2.4.1.8 55
-
 15 min, pH 7.0, 70% of the original activity is retained Paenibacillus sp.

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.4.1.8 3.5
-
 beta-D-glucose 1-phosphate 50°C, pH 7.0, mutant enzyme E487Q Paenibacillus sp.
2.4.1.8 3.8
-
 D-glucose 50°C, pH 7.0, mutant enzyme E487Q Paenibacillus sp.
2.4.1.8 12.9
-
 phosphate 50°C, pH 7.0, wild-type enzyme Paenibacillus sp.
2.4.1.8 17.3
-
 maltose 50°C, pH 7.0, wild-type enzyme Paenibacillus sp.
2.4.1.8 43.5
-
 beta-D-glucose 1-phosphate 50°C, pH 7.0, wild-type enzyme Paenibacillus sp.
2.4.1.8 49.4
-
 D-glucose 50°C, pH 7.0, wild-type enzyme Paenibacillus sp.

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.4.1.8 6
-
 glycosynthesis Paenibacillus sp.
2.4.1.8 7 7.5 phosphorolysis Paenibacillus sp.

pH Range

EC Number pH Minimum pH Maximum Comment Organism
2.4.1.8 5 8 more than 50% of maximal activity, phosphorolysis Paenibacillus sp.
2.4.1.8 6 7 more than 50% of maximal activity, glycosynthesis Paenibacillus sp.

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
2.4.1.8 5.5 6 15 min, 50°C, most stable in the pH-range 5.5-6.0 Paenibacillus sp.
2.4.1.8 5.5 7 15 min, 50°C, retains 50% of the original activity in the pH-range 5.5-7.0 Paenibacillus sp.