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Literature summary extracted from

  • Bozic, N.; Ivanovic, J.; Nenadovic, V.; Bergstroem, J.; Larsson, T.; Vujcic, Z.
    Purification and properties of major midgut leucyl aminopeptidase of Morimus funereus (Coleoptera, Cerambycidae) larvae (2008), Comp. Biochem. Physiol. B, 149, 454-462.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
3.4.11.1 EGTA 0.1-10 mM, 20% stimulation Morimus funereus
3.4.11.1 imidazole 20 mM, activates enzyme activity by 8% Morimus funereus

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.4.11.1 1,10-phenanthroline non-competitive Morimus funereus
3.4.11.1 citrate 10 mM and 20 mM, 15% inhibition Morimus funereus
3.4.11.1 cysteine competitive Morimus funereus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.4.11.1 0.17
-
 Met-4-nitroanilide pH 7.5, 30°C Morimus funereus
3.4.11.1 0.22
-
 Leu-4-nitroanilide pH 7.5, 30°C Morimus funereus
3.4.11.1 0.59
-
 Lys-4-nitroanilide pH 7.5, 30°C Morimus funereus
3.4.11.1 0.96
-
 Ala-4-nitroanilide pH 7.5, 30°C Morimus funereus

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.4.11.1 Mg2+ activity is increased at 0.05 mM, inhibition at 0.5 mM Morimus funereus
3.4.11.1 Mn2+ activity is increased at 0.05 mM, inhibition at 0.5 mM Morimus funereus
3.4.11.1 Zn2+ 10 nM activates over 30%, inhibits above 0.005 mM Morimus funereus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.4.11.1 67000
-
 6 * 67000, SDS-PAGE Morimus funereus
3.4.11.1 400000
-
 gel filtration Morimus funereus

Organism

EC Number Organism UniProt Comment Textmining
3.4.11.1 Morimus funereus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.4.11.1
-
 Morimus funereus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.4.11.1 larva midgut Morimus funereus
-
3.4.11.1 midgut larva Morimus funereus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.4.11.1 1108
-
-
 Morimus funereus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.4.11.1 Ala-4-nitroanilide + H2O
-
 Morimus funereus Ala + 4-nitroaniline
-
 ?
3.4.11.1 Leu-4-nitroanilide + H2O
-
 Morimus funereus Leu + 4-nitroaniline
-
 ?
3.4.11.1 Lys-4-nitroanilide + H2O
-
 Morimus funereus Lys + 4-nitroaniline
-
 ?
3.4.11.1 Met-4-nitroanilide + H2O
-
 Morimus funereus Met + 4-nitroaniline
-
 ?

Subunits

EC Number Subunits Comment Organism
3.4.11.1 hexamer 6 * 67000, SDS-PAGE Morimus funereus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.4.11.1 7.5
-
-
 Morimus funereus

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.4.11.1 6.5 9 pH 6.5: about 60% of maximal activity, pH 9.0: about 40% of maximal activity Morimus funereus

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
3.4.11.1 0.12
-
 1,10-phenanthroline
-
 Morimus funereus
3.4.11.1 0.31
-
 cysteine
-
 Morimus funereus

pI Value

EC Number Organism Comment pI Value Maximum pI Value
3.4.11.1 Morimus funereus isoelectric focusing
-
 6.2

IC50 Value

EC Number IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
3.4.11.1 0.18
-
-
 Morimus funereus 1,10-phenanthroline
3.4.11.1 0.47
-
-
 Morimus funereus cysteine