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Literature summary extracted from
- Disaccharidase activities in camel small intestine: Biochemical investigations of maltase-glucoamylase activity (2007), Comp. Biochem. Physiol. B, 146B, 124-130.
No PubMed abstract available
Inhibitors
| EC Number | Inhibitors | Comment | Organism | Structure |
|---|---|---|---|---|
| 3.2.1.20 | 1,10-phenanthroline | no inhibition of isozyme MG4, 22.5 inhibition of isozyme MG6 | Camelus dromedarius |  |
| 3.2.1.20 | 4-chloromercuribenzoate | 95% inhibition of isozyme MG4, no inhibition of isozyme MG6 | Camelus dromedarius | |
| 3.2.1.20 | 4-hydroxymercuribenzoate | 86% inhibition of isozyme MG4, 22% inhibition of isozyme MG6 | Camelus dromedarius | |
| 3.2.1.20 | DTNB | 66% inhibition of isozyme MG4, 41% inhibition of isozyme MG6 | Camelus dromedarius | |
| 3.2.1.20 | Hg2+ | - |
Camelus dromedarius | |
| 3.2.1.20 | iodoacetamide | 13% inhibition of isozyme MG4, 9% inhibition of isozyme MG6 | Camelus dromedarius | |
| 3.2.1.20 | NEM | 79% inhibition of isozyme MG4, no inhibition of isozyme MG6 | Camelus dromedarius | |
| 3.2.1.20 | PMSF | no inhibition of isozyme MG4, 47% inhibition of isozyme MG6 | Camelus dromedarius | |
| 3.2.1.20 | sucrose | inhibits the maltase activity of the enzyme | Camelus dromedarius | |
| 3.2.1.20 | Trypsin inhibitor | 17% inhibition of isozyme MG4, no inhibition of isozyme MG6 | Camelus dromedarius |
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 3.2.1.20 | 13.3 | - |
maltose | pH 5.5, 37°C, isozyme MG4 | Camelus dromedarius | |
| 3.2.1.20 | 20 | - |
maltose | pH 5.5, 37°C, isozyme MG6 | Camelus dromedarius | |
Metals/Ions
| EC Number | Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|---|
| 3.2.1.20 | additional information | only marginal effects of Ca2+, Mg2+, Mn2+, Co2+, and Fe3+ on the enzyme | Camelus dromedarius |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 3.2.1.20 | 140000 | - |
isozyme MG4, gel filtration | Camelus dromedarius |
| 3.2.1.20 | 160000 | - |
isozyme MG6, gel filtration | Camelus dromedarius |
Natural Substrates/ Products (Substrates)
| EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.2.1.20 | additional information | Camelus dromedarius | enzymic digestion profile determination for camel small intestine, overview | ? | - |
? | |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 3.2.1.20 | Camelus dromedarius | - |
- |
- |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 3.2.1.20 | native isozymes from small intestine by anion exchange chromatography and gel filtration, isozyme MG4 9fold, isozyme MG6 24fold | Camelus dromedarius |
Source Tissue
| EC Number | Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|---|
| 3.2.1.20 | small intestine | isozymes MG4 and MG6 | Camelus dromedarius | - |
Specific Activity [micromol/min/mg]
| EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|---|
| 3.2.1.20 | 0.024 | - |
glucoamylase activity with substrate starch, small intestine | Camelus dromedarius |
| 3.2.1.20 | 0.043 | - |
maltase activity with substrate maltose, small intestine | Camelus dromedarius |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.2.1.20 | alpha-cyclodextrin + H2O | low activity with isozymes MG4 and MG6 | Camelus dromedarius | alpha-D-glucose | - |
? | |
| 3.2.1.20 | amylopectin + H2O | - |
Camelus dromedarius | alpha-D-glucose | - |
? | |
| 3.2.1.20 | glycogen + H2O | - |
Camelus dromedarius | alpha-D-glucose | - |
? | |
| 3.2.1.20 | maltose + H2O | preferred substrate of isozymes MG4 and MG6 | Camelus dromedarius | alpha-D-glucose + D-glucose | - |
? | |
| 3.2.1.20 | additional information | enzymic digestion profile determination for camel small intestine, overview | Camelus dromedarius | ? | - |
? | |
| 3.2.1.20 | additional information | poor activity with sucrose, dextrine, lactose, and no activity with xylose | Camelus dromedarius | ? | - |
? | |
| 3.2.1.20 | starch + H2O | - |
Camelus dromedarius | alpha-D-glucose | - |
? | |
Subunits
| EC Number | Subunits | Comment | Organism |
|---|---|---|---|
| 3.2.1.20 | monomer | 1 * 140000, isozyme MG4, SDS-PAGE, 1 * 160000, isozyme MG6, SDS-PAGE | Camelus dromedarius |
Synonyms
| EC Number | Synonyms | Comment | Organism |
|---|---|---|---|
| 3.2.1.20 | maltase-glucoamylase | - |
Camelus dromedarius |
Temperature Optimum [°C]
| EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 3.2.1.20 | 40 | - |
isozyme MG6 | Camelus dromedarius |
| 3.2.1.20 | 50 | - |
isozyme MG4 | Camelus dromedarius |
Temperature Stability [°C]
| EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 3.2.1.20 | 40 | - |
isozyme MG6, stable up to | Camelus dromedarius |
| 3.2.1.20 | 50 | - |
isozyme MG4, stable up to, strong decrease in activity of isozyme MG6 | Camelus dromedarius |
| 3.2.1.20 | 60 | - |
strong decrease in activity of isozyme MG4 | Camelus dromedarius |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 3.2.1.20 | 7 | - |
isozymes MG4 and MG6 | Camelus dromedarius |
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Release 2023.1 (January 2023)
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