EC Number | Cloned (Comment) | Organism |
---|---|---|
3.1.2.2 | expression in Escherichia coli | Mycobacterium tuberculosis |
EC Number | Crystallization (Comment) | Organism |
---|---|---|
3.1.2.2 | hanging-drop vapor-diffusion method, crystal structure to 2.3 A resolution. FcoT crystals, produced in the presence of the substrate dodecenoyl-CoA, are cubic and belong to space group I2(1)3, with one molecule in the asymmetric unit | Mycobacterium tuberculosis |
EC Number | Protein Variants | Comment | Organism |
---|---|---|---|
3.1.2.2 | E77Q | mutation decreased the enzyme activity to less than 10fold of wild-type | Mycobacterium tuberculosis |
3.1.2.2 | H72A | mutation decreased the enzyme activity to less than 10fold of wild-type | Mycobacterium tuberculosis |
3.1.2.2 | N74A | mutation abolishes the activity of the enzyme | Mycobacterium tuberculosis |
3.1.2.2 | N83A | mutation decreased the enzyme activity to less than 10fold of wild-type | Mycobacterium tuberculosis |
3.1.2.2 | Y33F | mutation abolishes the activity of the enzyme | Mycobacterium tuberculosis |
3.1.2.2 | Y66F | mutation abolishes the activity of the enzyme | Mycobacterium tuberculosis |
3.1.2.2 | Y87F | mutation decreased the enzyme activity to less than 10fold of wild-type | Mycobacterium tuberculosis |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
3.1.2.2 | 0.01187 | - |
palmitoyl-CoA | pH 7.5 | Mycobacterium tuberculosis | |
3.1.2.2 | 0.01913 | - |
dodecanoyl-CoA | pH 7.5 | Mycobacterium tuberculosis | |
3.1.2.2 | 0.03852 | - |
lauroyl-CoA | pH 7.5 | Mycobacterium tuberculosis | |
3.1.2.2 | 0.06318 | - |
hexanoyl-CoA | pH 7.5 | Mycobacterium tuberculosis | |
3.1.2.2 | 0.08735 | - |
crotonyl-CoA | pH 7.5 | Mycobacterium tuberculosis | |
3.1.2.2 | 0.1028 | - |
stearoyl-CoA | pH 7.5 | Mycobacterium tuberculosis | |
3.1.2.2 | 0.272 | - |
benzoyl-CoA | pH 7.5 | Mycobacterium tuberculosis |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
3.1.2.2 | Mycobacterium tuberculosis | P9WM67 | - |
- |
3.1.2.2 | Mycobacterium tuberculosis H37Rv | P9WM67 | - |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
3.1.2.2 | - |
Mycobacterium tuberculosis |
EC Number | Source Tissue | Comment | Organism | Textmining |
---|
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
3.1.2.2 | benzoyl-CoA + H2O | - |
Mycobacterium tuberculosis | CoA + benzoate | - |
? | |
3.1.2.2 | crotonyl-CoA + H2O | - |
Mycobacterium tuberculosis | CoA + crotonate | - |
? | |
3.1.2.2 | crotonyl-CoA + H2O | - |
Mycobacterium tuberculosis H37Rv | CoA + crotonate | - |
? | |
3.1.2.2 | dodecanoyl-CoA + H2O | - |
Mycobacterium tuberculosis | CoA + dodecanoate | - |
? | |
3.1.2.2 | dodecanoyl-CoA + H2O | - |
Mycobacterium tuberculosis H37Rv | CoA + dodecanoate | - |
? | |
3.1.2.2 | hexanoyl-CoA + H2O | - |
Mycobacterium tuberculosis | CoA + hexanoate | - |
? | |
3.1.2.2 | hexanoyl-CoA + H2O | - |
Mycobacterium tuberculosis H37Rv | CoA + hexanoate | - |
? | |
3.1.2.2 | lauroyl-CoA + H2O | - |
Mycobacterium tuberculosis | CoA + laurate | - |
? | |
3.1.2.2 | palmitoyl-CoA + H2O | - |
Mycobacterium tuberculosis | CoA + palmitate | - |
? | |
3.1.2.2 | palmitoyl-CoA + H2O | - |
Mycobacterium tuberculosis H37Rv | CoA + palmitate | - |
? | |
3.1.2.2 | stearoyl-CoA + H2O | - |
Mycobacterium tuberculosis | CoA + stearate | - |
? | |
3.1.2.2 | stearoyl-CoA + H2O | - |
Mycobacterium tuberculosis H37Rv | CoA + stearate | - |
? |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
3.1.2.2 | FcoT | - |
Mycobacterium tuberculosis |
3.1.2.2 | long-chain fatty acyl-CoA thioesterase | - |
Mycobacterium tuberculosis |
EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
3.1.2.2 | 0.004 | - |
benzoyl-CoA | pH 7.5 | Mycobacterium tuberculosis | |
3.1.2.2 | 0.0078 | - |
crotonyl-CoA | pH 7.5 | Mycobacterium tuberculosis | |
3.1.2.2 | 0.01 | - |
dodecanoyl-CoA | pH 7.5 | Mycobacterium tuberculosis | |
3.1.2.2 | 0.012 | - |
hexanoyl-CoA | pH 7.5 | Mycobacterium tuberculosis | |
3.1.2.2 | 0.015 | - |
lauroyl-CoA | pH 7.5 | Mycobacterium tuberculosis | |
3.1.2.2 | 0.019 | - |
stearoyl-CoA | pH 7.5 | Mycobacterium tuberculosis | |
3.1.2.2 | 0.037 | - |
palmitoyl-CoA | pH 7.5 | Mycobacterium tuberculosis |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
3.1.2.2 | 7 | 8.5 | below pH 7 there is no detectable activity. In the range of pH 78.5, the activity shows a linear phase and reaches a plateau at pH 8.5 | Mycobacterium tuberculosis |
EC Number | pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|---|
3.1.2.2 | 7 | 8.5 | below pH 7 there is no detectable activity. In the range of pH 78.5, the activity shows a linear phase and reaches a plateau at pH 8.5 | Mycobacterium tuberculosis |