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Literature summary extracted from

  • Wang, F.; Langley, R.; Gulten, G.; Wang, L.; Sacchettini, J.C.
    Identification of a type III thioesterase reveals the function of an operon crucial for Mtb virulence (2007), Chem. Biol., 14, 543-551.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.1.2.2 expression in Escherichia coli Mycobacterium tuberculosis

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
3.1.2.2 hanging-drop vapor-diffusion method, crystal structure to 2.3 A resolution. FcoT crystals, produced in the presence of the substrate dodecenoyl-CoA, are cubic and belong to space group I2(1)3, with one molecule in the asymmetric unit Mycobacterium tuberculosis

Protein Variants

EC Number Protein Variants Comment Organism
3.1.2.2 E77Q mutation decreased the enzyme activity to less than 10fold of wild-type Mycobacterium tuberculosis
3.1.2.2 H72A mutation decreased the enzyme activity to less than 10fold of wild-type Mycobacterium tuberculosis
3.1.2.2 N74A mutation abolishes the activity of the enzyme Mycobacterium tuberculosis
3.1.2.2 N83A mutation decreased the enzyme activity to less than 10fold of wild-type Mycobacterium tuberculosis
3.1.2.2 Y33F mutation abolishes the activity of the enzyme Mycobacterium tuberculosis
3.1.2.2 Y66F mutation abolishes the activity of the enzyme Mycobacterium tuberculosis
3.1.2.2 Y87F mutation decreased the enzyme activity to less than 10fold of wild-type Mycobacterium tuberculosis

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.1.2.2 0.01187
-
palmitoyl-CoA pH 7.5 Mycobacterium tuberculosis
3.1.2.2 0.01913
-
dodecanoyl-CoA pH 7.5 Mycobacterium tuberculosis
3.1.2.2 0.03852
-
lauroyl-CoA pH 7.5 Mycobacterium tuberculosis
3.1.2.2 0.06318
-
hexanoyl-CoA pH 7.5 Mycobacterium tuberculosis
3.1.2.2 0.08735
-
crotonyl-CoA pH 7.5 Mycobacterium tuberculosis
3.1.2.2 0.1028
-
stearoyl-CoA pH 7.5 Mycobacterium tuberculosis
3.1.2.2 0.272
-
benzoyl-CoA pH 7.5 Mycobacterium tuberculosis

Organism

EC Number Organism UniProt Comment Textmining
3.1.2.2 Mycobacterium tuberculosis P9WM67
-
-
3.1.2.2 Mycobacterium tuberculosis H37Rv P9WM67
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.1.2.2
-
Mycobacterium tuberculosis

Source Tissue

EC Number Source Tissue Comment Organism Textmining

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.2.2 benzoyl-CoA + H2O
-
Mycobacterium tuberculosis CoA + benzoate
-
?
3.1.2.2 crotonyl-CoA + H2O
-
Mycobacterium tuberculosis CoA + crotonate
-
?
3.1.2.2 crotonyl-CoA + H2O
-
Mycobacterium tuberculosis H37Rv CoA + crotonate
-
?
3.1.2.2 dodecanoyl-CoA + H2O
-
Mycobacterium tuberculosis CoA + dodecanoate
-
?
3.1.2.2 dodecanoyl-CoA + H2O
-
Mycobacterium tuberculosis H37Rv CoA + dodecanoate
-
?
3.1.2.2 hexanoyl-CoA + H2O
-
Mycobacterium tuberculosis CoA + hexanoate
-
?
3.1.2.2 hexanoyl-CoA + H2O
-
Mycobacterium tuberculosis H37Rv CoA + hexanoate
-
?
3.1.2.2 lauroyl-CoA + H2O
-
Mycobacterium tuberculosis CoA + laurate
-
?
3.1.2.2 palmitoyl-CoA + H2O
-
Mycobacterium tuberculosis CoA + palmitate
-
?
3.1.2.2 palmitoyl-CoA + H2O
-
Mycobacterium tuberculosis H37Rv CoA + palmitate
-
?
3.1.2.2 stearoyl-CoA + H2O
-
Mycobacterium tuberculosis CoA + stearate
-
?
3.1.2.2 stearoyl-CoA + H2O
-
Mycobacterium tuberculosis H37Rv CoA + stearate
-
?

Synonyms

EC Number Synonyms Comment Organism
3.1.2.2 FcoT
-
Mycobacterium tuberculosis
3.1.2.2 long-chain fatty acyl-CoA thioesterase
-
Mycobacterium tuberculosis

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.1.2.2 0.004
-
benzoyl-CoA pH 7.5 Mycobacterium tuberculosis
3.1.2.2 0.0078
-
crotonyl-CoA pH 7.5 Mycobacterium tuberculosis
3.1.2.2 0.01
-
dodecanoyl-CoA pH 7.5 Mycobacterium tuberculosis
3.1.2.2 0.012
-
hexanoyl-CoA pH 7.5 Mycobacterium tuberculosis
3.1.2.2 0.015
-
lauroyl-CoA pH 7.5 Mycobacterium tuberculosis
3.1.2.2 0.019
-
stearoyl-CoA pH 7.5 Mycobacterium tuberculosis
3.1.2.2 0.037
-
palmitoyl-CoA pH 7.5 Mycobacterium tuberculosis

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.1.2.2 7 8.5 below pH 7 there is no detectable activity. In the range of pH 7–8.5, the activity shows a linear phase and reaches a plateau at pH 8.5 Mycobacterium tuberculosis

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.1.2.2 7 8.5 below pH 7 there is no detectable activity. In the range of pH 7–8.5, the activity shows a linear phase and reaches a plateau at pH 8.5 Mycobacterium tuberculosis