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Literature summary extracted from

  • Vandeput, F.; Backers, K.; Villeret, V.; Pesesse, X.; Erneux, C.
    The influence of anionic lipids on SHIP2 phosphatidylinositol 3,4,5-trisphosphate 5-phosphatase activity (2006), Cell. Signal., 18, 2193-2199.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
3.1.3.36 additional information in contrast to phosphatidylserine SH2 domain containing inositol 5-phosphatase 2 (SHIP2) no stimulation by phosphatidylserine (PtdSer) Homo sapiens
3.1.3.36 phosphatidylcholine vesicles of phosphatidylcholine only stimulate SHIP2 activity by 2fold. This effect is specific for di-C8 and di-C16 fatty acids of D-myo-phosphatidylinositol 3,4,5-trisphosphate as substrate Homo sapiens
3.1.3.36 phosphatidylserine vesicles of phosphatidylserine (PtdSer) greatly stimulate SHIP2. This effect is specific for di-C8 and di-C16 fatty acids of D-myo-phosphatidylinositol 3,4,5-trisphosphate as substrate Homo sapiens
3.1.3.86 additional information SHIP2 enzyme activity with phosphatidylinositol 3,4,5-trisphosphate is 11fold stimulated in the presence of 0.02 mM vesicles of phosphatidylserine. 0.2 mM vesicles of phosphatidylcholine stimulate 2fold phosphatidylinositol 3,4,5-trisphosphate phosphatase activity of SHIP2 Homo sapiens

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.1.3.36 expressed in Escherichia coli as a GST-tagged protein Homo sapiens
3.1.3.86 expressed in Escherichia coli BL21(DE3) cells Homo sapiens

Protein Variants

EC Number Protein Variants Comment Organism
3.1.3.36 additional information a minimal catalytic construct D421-T730 is also sensitive to stimulation by phosphatidylserine Homo sapiens

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.1.3.36 Mg2+
-
 Homo sapiens

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.1.3.86 1-phosphatidyl-1D-myo-inositol 3,4,5-trisphosphate + H2O Homo sapiens in vivo substrate of SHIP2 1-phosphatidyl-1D-myo-inositol 3,4-bisphosphate + phosphate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.1.3.36 Homo sapiens
-
-
-
3.1.3.86 Homo sapiens
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.1.3.86 glutathione-agarose resin column chromatography Homo sapiens

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.1.3.36 kidney
-
 Homo sapiens
-
3.1.3.36 skeletal muscle
-
 Homo sapiens
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.3.36 1D-myo-inositol 1,3,4,5-tetrasphosphate + H2O
-
 Homo sapiens 1D-myo-inositol 1,3,4-trisphosphate + phosphate
-
 ?
3.1.3.36 D-myo-phosphatidylinositol 3,4,5-trisphosphate reaction is not stimulated by phosphatidylserine Homo sapiens D-myo-phosphatidylinositol 3,4-bisphosphate + phosphate
-
 ?
3.1.3.36 D-myo-phosphatidylinositol 3,4,5-trisphosphate reaction is strongly stimulated by phosphatidylserine Homo sapiens D-myo-phosphatidylinositol 3,4-bisphosphate + phosphate
-
 ?
3.1.3.86 1-phosphatidyl-1D-myo-inositol 3,4,5-trisphosphate + H2O in vivo substrate of SHIP2 Homo sapiens 1-phosphatidyl-1D-myo-inositol 3,4-bisphosphate + phosphate
-
 ?
3.1.3.86 1-phosphatidyl-1D-myo-inositol 3,4,5-trisphosphate + H2O in vitro substrate of SHIP2 Homo sapiens 1-phosphatidyl-1D-myo-inositol 3,4-bisphosphate + phosphate
-
 ?
3.1.3.86 inositol 1,3,4,5-tetrakisphosphate + H2O in vitro substrate of SHIP2 Homo sapiens ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.1.3.36 SH2 domain containing inositol 5-phosphatase 2
-
 Homo sapiens
3.1.3.36 SHIP2
-
 Homo sapiens
3.1.3.36 skeletal muscle and kidney enriched inositol phosphatase
-
 Homo sapiens
3.1.3.36 SKIP
-
 Homo sapiens
3.1.3.86 phosphatidylinositol 3,4,5-trisphosphate 5-phosphatase
-
 Homo sapiens
3.1.3.86 PtdIns(3,4,5)P3 5-phosphatase
-
 Homo sapiens
3.1.3.86 SH2 domain containing inositol 5-phosphatase 2
-
 Homo sapiens
3.1.3.86 SHIP2
-
 Homo sapiens

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.1.3.36 37
-
 assay at Homo sapiens

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.1.3.36 7.4
-
 assay at Homo sapiens

General Information

EC Number General Information Comment Organism
3.1.3.86 metabolism the enzyme participates in the insulin signalling pathway in vivo Homo sapiens