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Literature summary extracted from

  • Suzuki, H.; Suzuki, K.; Inoue, A.; Ojima, T.
    A novel oligoalginate lyase from abalone, Haliotis discus hannai, that releases disaccharide from alginate polymer in an exolytic manner (2006), Carbohydr. Res., 341, 1809-1819.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
4.2.2.3 expression in Escherichia coli Haliotis discus hannai

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
4.2.2.3 32000
-
 x * 32000, SDS-PAGE Haliotis discus hannai

Organism

EC Number Organism UniProt Comment Textmining
4.2.2.3 Haliotis discus hannai
-
-
-
4.2.2.3 Haliotis discus hannai Q25C77
-
-
4.2.2.26 Haliotis discus hannai
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
4.2.2.3
-
 Haliotis discus hannai

Source Tissue

EC Number Source Tissue Comment Organism Textmining
4.2.2.3 digestive juice
-
 Haliotis discus hannai
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
4.2.2.3 1786
-
 pH 7.0, 30°C Haliotis discus hannai

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.2.2.3 alginate HdAlex can degrade not only unsaturated trisaccharide but also alginate and mannuronate-rich polymers. HdAlex degrades the alginate polymer in an exolytic manner Haliotis discus hannai ?
-
 ?
4.2.2.3 alginate + H2O
-
 Haliotis discus hannai ?
-
 ?
4.2.2.3 poly(beta-(1->4)-D-mannuronate) 530% of the activity with alginate Haliotis discus hannai an unsaturated mannuronate disaccharide enzyme acts as an exolytic lyase, releasing unsaturated disaccharide directly from the poly(M)-rich substrate. It splits the second glycosyl linkage from the reducing terminus of the trisaccharide, the resulting disaccharide is not further degraded ?
4.2.2.3 poly(beta-(1->4)-D-mannuronic acid/alpha-(1->4)-L-guluronic acid) 45% of the activity with alginate Haliotis discus hannai ?
-
 ?

Subunits

EC Number Subunits Comment Organism
4.2.2.3 ? x * 32000, SDS-PAGE Haliotis discus hannai

Synonyms

EC Number Synonyms Comment Organism
4.2.2.3 HdAlex
-
 Haliotis discus hannai
4.2.2.3 hdalex-1
-
 Haliotis discus hannai

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
4.2.2.3 42
-
-
 Haliotis discus hannai

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
4.2.2.3 30 50 30°C: about 50% of maximal activity, 50°C: about 10% of maximal activity Haliotis discus hannai

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
4.2.2.3 7.1
-
-
 Haliotis discus hannai

pH Range

EC Number pH Minimum pH Maximum Comment Organism
4.2.2.3 5.5 8 pH 5.5: about 30% of maximal activity, pH 8.0: about 60% of maximal activity Haliotis discus hannai

General Information

EC Number General Information Comment Organism
4.2.2.3 physiological function the preferable substrate of enzyme Hdalex in the abalone digestive fluid is the unsaturated trisaccharide produced by alginate lyase Hdaly, EC 4.2.2.3 Haliotis discus hannai