EC Number | Cloned (Comment) | Organism |
---|---|---|
3.2.1.14 | expression of wild-type and mutant enzymes in Escherichia coli | Vibrio harveyi |
EC Number | Protein Variants | Comment | Organism |
---|---|---|---|
3.2.1.14 | S33W | strong increase in binding activity towards crystalline alpha-chitin and colloidal chitin. Mutation has no effect on specific hydrolyzing activity against p-nitrophenyl-N,N'-diacetylchitobiose, or the catalytic efficiency against chitohexaose | Vibrio harveyi |
3.2.1.14 | W231A | strong increase in binding activity towards crystalline alpha-chitin and colloidal chitin. Mutant displayed reduced hydrolytic activity. Mutation has no effect on specific hydrolyzing activity against p-nitrophenyl-N,N'-diacetylchitobiose, or the catalytic efficiency against chitohexaose | Vibrio harveyi |
3.2.1.14 | W70A | strong decrease in binding activity towards crystalline alpha-chitin and colloidal chitin. Mutant enzyme shows severe loss in hydrolytic activities. Mutation has no effect on specific hydrolyzing activity against p-nitrophenyl-N,N'-diacetylchitobiose, or the catalytic efficiency against chitohexaose | Vibrio harveyi |
3.2.1.14 | Y245W | strong decrease in binding activity towards crystalline alpha-chitin and colloidal chitin. Mutant displayes enhanced hydrolytic activity. Mutation has no effect on specific hydrolyzing activity against p-nitrophenyl-N,N'-diacetylchitobiose, or the catalytic efficiency against chitohexaose | Vibrio harveyi |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
3.2.1.14 | 0.163 | - |
chitohexaose | pH 5.5, 37°C, mutant enzyme W231F | Vibrio harveyi | |
3.2.1.14 | 0.171 | - |
chitohexaose | pH 5.5, 37°C, mutant enzyme S33A | Vibrio harveyi | |
3.2.1.14 | 0.185 | - |
chitohexaose | pH 5.5, 37°C, mutant enzyme W70A | Vibrio harveyi | |
3.2.1.14 | 0.189 | - |
chitohexaose | pH 5.5, 37°C, mutant enzyme W231A | Vibrio harveyi | |
3.2.1.14 | 0.201 | - |
chitohexaose | pH 5.5, 37°C, mutant enzyme Y245W | Vibrio harveyi | |
3.2.1.14 | 0.21 | - |
chitohexaose | pH 5.5, 37°C, mutant enzyme S33W | Vibrio harveyi | |
3.2.1.14 | 0.218 | - |
chitohexaose | pH 5.5, 37°C, wild-type enzyme | Vibrio harveyi |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
3.2.1.14 | Vibrio harveyi | - |
- |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
3.2.1.14 | - |
Vibrio harveyi |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
3.2.1.14 | chitin + H2O | low activity with crystallin chitin, high activity with colloidal chitin | Vibrio harveyi | ? | - |
? | |
3.2.1.14 | chitohexaose + H2O | - |
Vibrio harveyi | ? | - |
? | |
3.2.1.14 | p-nitrophenyl-N,N'-diacetylchitobiose + H2O | - |
Vibrio harveyi | p-nitrophenol + N,N'-diacetylchitobiose | - |
? |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
3.2.1.14 | chitinase A | - |
Vibrio harveyi |
EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
3.2.1.14 | 0.4 | - |
chitin | pH 5.5, 37°C, colloidal chitin, mutant enzym W70A | Vibrio harveyi | |
3.2.1.14 | 0.6 | - |
chitin | pH 5.5, 37°C, colloidal chitin, mutant enzym W231A | Vibrio harveyi | |
3.2.1.14 | 0.9 | - |
chitin | pH 5.5, 37°C, colloidal chitin, mutant enzym S33A | Vibrio harveyi | |
3.2.1.14 | 0.9 | - |
chitin | pH 5.5, 37°C, colloidal chitin, mutant enzym W231F | Vibrio harveyi | |
3.2.1.14 | 1.2 | - |
chitin | pH 5.5, 37°C, colloidal chitin, wild-type enzyme | Vibrio harveyi | |
3.2.1.14 | 1.4 | - |
chitin | pH 5.5, 37°C, colloidal chitin, mutant enzym S33W | Vibrio harveyi | |
3.2.1.14 | 1.7 | - |
chitin | pH 5.5, 37°C, colloidal chitin, mutant enzym Y245W | Vibrio harveyi | |
3.2.1.14 | 2.4 | - |
chitohexaose | pH 5.5, 37°C, mutant enzyme W231F | Vibrio harveyi | |
3.2.1.14 | 2.5 | - |
chitohexaose | pH 5.5, 37°C, mutant enzyme W70A | Vibrio harveyi | |
3.2.1.14 | 2.6 | - |
chitohexaose | pH 5.5, 37°C, mutant enzyme S33A | Vibrio harveyi | |
3.2.1.14 | 2.6 | - |
chitohexaose | pH 5.5, 37°C, mutant enzyme W231A | Vibrio harveyi | |
3.2.1.14 | 2.6 | - |
chitohexaose | pH 5.5, 37°C, mutant enzyme Y245W | Vibrio harveyi | |
3.2.1.14 | 2.8 | - |
chitohexaose | pH 5.5, 37°C, mutant enzyme S33W | Vibrio harveyi | |
3.2.1.14 | 2.9 | - |
chitohexaose | pH 5.5, 37°C, wild-type enzyme | Vibrio harveyi |