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Literature summary extracted from

  • Hutchinson, M.H.; Chase, H.A.
    Intensified process for the purification of an enzyme from inclusion bodies using integrated expanded bed adsorption and refolding (2006), Biotechnol. Prog., 22, 1187-1193.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
5.3.3.1 expression of C-terminally His6-tagged enzyme as insoluble protein in inclusion bodies in Escherichia coli strain BLR(DE3) Comamonas testosteroni

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
5.3.3.1 5-Androstene-3,17-dione Comamonas testosteroni
-
4-Androstene-3,17-dione
-
?

Organism

EC Number Organism UniProt Comment Textmining
5.3.3.1 Comamonas testosteroni P00947 i.e. Pseudomonas testosteroni
-

Purification (Commentary)

EC Number Purification (Comment) Organism
5.3.3.1 recombinant C-terminally His6-tagged enzyme from Escherichia coli strain BLR(DE3) to homogeneity by expanded bed adsorption column utilizing immobilized metal affinity chromatography with denaturant concentration in the feed stream from 8 to 0 M urea, chemical extraction from cells, method optimization and validation, overview Comamonas testosteroni

Renatured (Commentary)

EC Number Renatured (Comment) Organism
5.3.3.1 repeated adsorptive refolding in an expanded bed with gradient change in the feed-stream composition from denaturing buffer containing 8 M urea and 50 mM potassium phosphate, pH 6.8 to the refolding buffer containing 50 mM potassium phosphate, pH 6.8, constant liquid feed rate, method evaluation, overview Comamonas testosteroni

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
5.3.3.1 5-Androstene-3,17-dione
-
Comamonas testosteroni 4-Androstene-3,17-dione
-
?

Synonyms

EC Number Synonyms Comment Organism
5.3.3.1 DELTA5-3-ketosteroid isomerase
-
Comamonas testosteroni
5.3.3.1 ketosteroid isomerase
-
Comamonas testosteroni
5.3.3.1 KSI
-
Comamonas testosteroni

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
5.3.3.1 30
-
assay at Comamonas testosteroni

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
5.3.3.1 7
-
assay at Comamonas testosteroni