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Literature summary extracted from

  • Khambhaty, Y.; Mody, K.; Jha, B.
    Purification and characterization of k-carrageenase from a novel gamma-proteobacterium, Pseudomonas elongata (MTCC 5261) syn. Microbulbifer elongatus comb. Nov (2007), Biotechnol. Bioprocess Eng., 12, 668-675.
No PubMed abstract available

General Stability

EC Number General Stability Organism
3.2.1.83 repeated freezing and thawing drastically reduces activity Microbulbifer elongatus

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.2.1.83 AlCl3 1 mM, 39.7% inhibition Microbulbifer elongatus
3.2.1.83 BaCl2 1 mM, 26.8% inhibition Microbulbifer elongatus
3.2.1.83 CaCl2 1 mM, 50.7% inhibition Microbulbifer elongatus
3.2.1.83 FeCl3 1 mM, 36.5% inhibition Microbulbifer elongatus
3.2.1.83 HgCl2 1 mM, 40.9% inhibition Microbulbifer elongatus
3.2.1.83 KCl 1 mM, 38% inhibition Microbulbifer elongatus
3.2.1.83 MgCl2 1 mM, 52.9% inhibition Microbulbifer elongatus
3.2.1.83 MnCl2 1 mM, 36% inhibition Microbulbifer elongatus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.2.1.83 additional information
-
 additional information Km-value for kappa-carrageenan is 6.66 mg/ml Microbulbifer elongatus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.2.1.83 extracellular
-
 Microbulbifer elongatus
-
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.1.83 128000
-
 gel filtrration Microbulbifer elongatus

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.83 Microbulbifer elongatus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.83
-
 Microbulbifer elongatus

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.2.1.83 426.2
-
-
 Microbulbifer elongatus

Storage Stability

EC Number Storage Stability Organism
3.2.1.83 20°C, 5 days, 40% loss of activity Microbulbifer elongatus
3.2.1.83 frozen, stable for 15 days Microbulbifer elongatus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.83 kappa-carrageenan + H2O
-
 Microbulbifer elongatus ?
-
 ?
3.2.1.83 additional information isolated kappa-carrageenase can successfully generate protoplasts of Kappaphycus alvarezii. No activity with lambda-carrageenan, ioro-carrageenan and LMP agarose Microbulbifer elongatus ?
-
 ?

Subunits

EC Number Subunits Comment Organism
3.2.1.83 monomer 1 * 128000, SDS-PAGE Microbulbifer elongatus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.83 40
-
-
 Microbulbifer elongatus

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.2.1.83 20
-
 40% loss of activity within 5 days, complete loss of activity within 12 days Microbulbifer elongatus
3.2.1.83 37
-
 5 days, 50% loss of activity Microbulbifer elongatus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.83 5.6
-
 and a second optimum at pH 7.7 Microbulbifer elongatus
3.2.1.83 7.7
-
 and a second optimum at pH 5.6 Microbulbifer elongatus

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.2.1.83 4.8 9 pH 4.8: about 70% of maximal activity, pH 9.0: about 60% of maximal activity Microbulbifer elongatus