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Literature summary extracted from

  • Nagahashi, Y.; Tazoe, M.; Hoshino, T.
    Cloning of the pyridoxine 5-phosphate phosphatase gene (pdxP) and vitamin B(6) production in pdxP recombinant Sinorhizobium meliloti (2008), Biosci. Biotechnol. Biochem., 72, 421-427.
    View publication on PubMed

Application

EC Number Application Comment Organism
3.1.3.74 drug development possibility of development of the pyridoxine production process by a metabolic engineering approach. In the future, overexpression of the pdxP gene in combination with another pyridoxine gene will be necessary to develop even more efficient processes for production of vitamin B6 Sinorhizobium meliloti

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.1.3.74 into pCRII-TOPO vector, 0.86 kb SmaI fragment from TOPOpdxP ligated into the SmaI site of pKK223-3, overexpression in Escherichia coli JM109. Overexpression in Sinorhizobium meliloti IFO 14782 recombinant with pVKPtacpdxP Sinorhizobium meliloti

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.1.3.74 26470
-
 sequence analysis Sinorhizobium meliloti

Organism

EC Number Organism UniProt Comment Textmining
3.1.3.74 Sinorhizobium meliloti A7BK78 IFO 14782
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.1.3.74 by gel filtration Sinorhizobium meliloti

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.1.3.74 additional information
-
 Sinorhizobium meliloti IFO 14782/pVKPtacpdxP shows PNP phosphatase activity 3.5times higher than that of the parent strain, when 2 mM pyridoxine 5'-phosphate is used as substrate. When pyridoxamine 5'-phosphate is used as substrate, the phosphatase activity of Sinorhizobium meliloti IFO 14782/pVKPtacpdxP is as low as that of the parent Sinorhizobium meliloti

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.3.74 additional information PNP phosphatase does not act on pyridoxamine 5'-phosphate Sinorhizobium meliloti ?
-
 ?
3.1.3.74 pyridoxine 5'-phosphate + H2O
-
 Sinorhizobium meliloti pyridoxine + phosphate
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.1.3.74 PNP phosphatase
-
 Sinorhizobium meliloti
3.1.3.74 pyridoxine 5'-phosphate phosphatase
-
 Sinorhizobium meliloti