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Literature summary extracted from

  • Tsuruta, H.; Hayashi, R.; Ohkawa, H.; Ohkatsu, N.; Morimoto, M.; Nishimoto, K.; Santou, S.; Aizono, Y.
    Characterisitics and gene cloning of phospholipase D of the psychrophile, Shewanella sp. (2007), Biosci. Biotechnol. Biochem., 71, 2534-2542.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.1.4.4
-
 Shewanella sp.

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.1.4.4 Al3+ 2.5 mM, complete loss of both hydrolytic activity and transphosphatidylation Shewanella sp.
3.1.4.4 Co2+ 2.5 mM, complete loss of both hydrolytic activity and transphosphatidylation Shewanella sp.
3.1.4.4 Cu2+ 2.5 mM, complete loss of both hydrolytic activity and transphosphatidylation Shewanella sp.
3.1.4.4 Fe3+ 2.5 mM, complete loss of both hydrolytic activity and transphosphatidylation Shewanella sp.
3.1.4.4 Mg2+ 2.5 mM, inhibitory to both hydrolytic activity and transphosphatidylation Shewanella sp.
3.1.4.4 Mn2+ 2.5 mM, complete loss of both hydrolytic activity and transphosphatidylation Shewanella sp.
3.1.4.4 Ni2+ 2.5 mM, complete loss of both hydrolytic activity and transphosphatidylation Shewanella sp.
3.1.4.4 Zn2+ 2.5 mM, complete loss of both hydrolytic activity and transphosphatidylation Shewanella sp.

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.1.4.4 Ca2+ presence of Ca2+ enhances 1.6fold hydrolytic activiy and 6fold transphosphatidylation Shewanella sp.

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.1.4.4 64000
-
 x * 64000, SDS-PAGE Shewanella sp.

Organic Solvent Stability

EC Number Organic Solvent Comment Organism
3.1.4.4 Ethanol enzyme shows high activity to the non-micelle form of phosphatidylcholine in an aequeous solution containing methanol, ethanol, iso-propanol, or n-propanol Shewanella sp.
3.1.4.4 isopropanol enzyme shows high activity to the non-micelle form of phosphatidylcholine in an aequeous solution containing methanol, ethanol, isopropanol, or n-propanol Shewanella sp.
3.1.4.4 Methanol assay in presence of 50% ethanol Shewanella sp.
3.1.4.4 n-propanol enzyme shows high activity to the non-micelle form of phosphatidylcholine in an aequeous solution containing methanol, ethanol, isopropanol, or n-propanol Shewanella sp.

Organism

EC Number Organism UniProt Comment Textmining
3.1.4.4 Shewanella sp.
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.1.4.4
-
 Shewanella sp.

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.1.4.4 0.076
-
 pH 7.8, 25°C, presence of 50% methanol Shewanella sp.

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.4.4 additional information enzyme exhibits a ratio of hydrolytic activity to transphosphatidylation of 2.5 Shewanella sp. ?
-
 ?
3.1.4.4 phosphatidylcholine + H2O high activity to the non-micelle form of phosphatidylcholine in an aequeous solution containing methanol, ethanol, isopropanol, or n-propanol. In absence of alcohol, hydrolytic activity is weak, and no transphosphatidylation activity is detected Shewanella sp. 1,2-diacylglycerophosphate + choline
-
 ?

Subunits

EC Number Subunits Comment Organism
3.1.4.4 ? x * 64000, SDS-PAGE Shewanella sp.

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.1.4.4 40
-
 presence of Ca2+ Shewanella sp.

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
3.1.4.4 10
-
 6.5% of maximum activity Shewanella sp.

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.1.4.4 30
-
 stable below Shewanella sp.
3.1.4.4 40
-
 rather labile above Shewanella sp.
3.1.4.4 60
-
 rapid loss of activity Shewanella sp.

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.1.4.4 7.8
-
-
 Shewanella sp.

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
3.1.4.4 5 8 enzyme is labile in the low pH range Shewanella sp.