BRENDA - Enzyme Database

Purification and characterization of phospholipase B from Candida utilis

Fujino, S.; Akiyama, D.; Akaboshi, S.; Fujita, T.; Watanabe, Y.; Tamai, Y.; Biosci. Biotechnol. Biochem. 70, 377-386 (2006)

Data extracted from this reference:

Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
3.1.1.5
Fe3+
at pH 3.0
Cyberlindnera jadinii
3.1.1.5
Hg2+
at pH 3.0
Cyberlindnera jadinii
Metals/Ions
EC Number
Metals/Ions
Commentary
Organism
Structure
3.1.1.5
Al3+
strong activation at pH 8.0, optimal concentration is 20 mM
Cyberlindnera jadinii
3.1.1.5
Co2+
pH 8.0, activates
Cyberlindnera jadinii
3.1.1.5
Cu2+
pH 8.0, activates
Cyberlindnera jadinii
3.1.1.5
Fe2+
pH 8.0, activates
Cyberlindnera jadinii
3.1.1.5
Fe3+
pH 8.0, activates
Cyberlindnera jadinii
3.1.1.5
Mn2+
pH 8.0, activates
Cyberlindnera jadinii
3.1.1.5
additional information
activity at pH 3.0 is independent on metal ions
Cyberlindnera jadinii
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
3.1.1.5
Cyberlindnera jadinii
Q7Z8L3
-
-
Posttranslational Modification
EC Number
Posttranslational Modification
Commentary
Organism
3.1.1.5
glycoprotein
-
Cyberlindnera jadinii
Purification (Commentary)
EC Number
Commentary
Organism
3.1.1.5
-
Cyberlindnera jadinii
Source Tissue
EC Number
Source Tissue
Commentary
Organism
Textmining
3.1.1.5
culture medium
-
Cyberlindnera jadinii
-
Specific Activity [micromol/min/mg]
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
3.1.1.5
28.72
-
-
Cyberlindnera jadinii
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
3.1.1.5
2-lysophosphatidylcholine + H2O
-
678790
Cyberlindnera jadinii
glycerophosphocholine + a fatty acid
-
-
-
?
3.1.1.5
lysophosphatidylethanolamine + H2O
-
678790
Cyberlindnera jadinii
fatty acid + glycerylphosphoethanolamine
-
-
-
?
3.1.1.5
phosphatidic acid + H2O
-
678790
Cyberlindnera jadinii
?
-
-
-
?
3.1.1.5
phosphatidylcholine + H2O
-
678790
Cyberlindnera jadinii
lysophosphatidylcholine + glycerophosphorylcholine + a fatty acid
-
-
-
?
3.1.1.5
phosphatidylethanolamine + H2O
-
678790
Cyberlindnera jadinii
lysophosphatidylethanolamine + glycerophosphorylethanolamine + a fatty acid
-
-
-
?
3.1.1.5
phosphatidylinositol + H2O
-
678790
Cyberlindnera jadinii
lysophophatidylinositol + a carboxylate
-
-
-
?
3.1.1.5
phosphatidylserine + H2O
-
678790
Cyberlindnera jadinii
lysophosphatidylserine + glycerophosphorylserine + a fatty acid
-
-
-
?
Subunits
EC Number
Subunits
Commentary
Organism
3.1.1.5
?
x * 90000-110000, SDS-PAGE
Cyberlindnera jadinii
Temperature Optimum [°C]
EC Number
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
3.1.1.5
40
-
at pH 3.0
Cyberlindnera jadinii
Temperature Stability [°C]
EC Number
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
3.1.1.5
80
-
complete loss of activity after incubation above 80°C
Cyberlindnera jadinii
pH Optimum
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
3.1.1.5
3
-
two pH-optima: pH 3.0 and pH 7.5
Cyberlindnera jadinii
3.1.1.5
7.5
-
two pH-optima: pH 3.0 and pH 7.5
Cyberlindnera jadinii
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
3.1.1.5
Fe3+
at pH 3.0
Cyberlindnera jadinii
3.1.1.5
Hg2+
at pH 3.0
Cyberlindnera jadinii
Metals/Ions (protein specific)
EC Number
Metals/Ions
Commentary
Organism
Structure
3.1.1.5
Al3+
strong activation at pH 8.0, optimal concentration is 20 mM
Cyberlindnera jadinii
3.1.1.5
Co2+
pH 8.0, activates
Cyberlindnera jadinii
3.1.1.5
Cu2+
pH 8.0, activates
Cyberlindnera jadinii
3.1.1.5
Fe2+
pH 8.0, activates
Cyberlindnera jadinii
3.1.1.5
Fe3+
pH 8.0, activates
Cyberlindnera jadinii
3.1.1.5
Mn2+
pH 8.0, activates
Cyberlindnera jadinii
3.1.1.5
additional information
activity at pH 3.0 is independent on metal ions
Cyberlindnera jadinii
Posttranslational Modification (protein specific)
EC Number
Posttranslational Modification
Commentary
Organism
3.1.1.5
glycoprotein
-
Cyberlindnera jadinii
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
3.1.1.5
-
Cyberlindnera jadinii
Source Tissue (protein specific)
EC Number
Source Tissue
Commentary
Organism
Textmining
3.1.1.5
culture medium
-
Cyberlindnera jadinii
-
Specific Activity [micromol/min/mg] (protein specific)
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
3.1.1.5
28.72
-
-
Cyberlindnera jadinii
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
3.1.1.5
2-lysophosphatidylcholine + H2O
-
678790
Cyberlindnera jadinii
glycerophosphocholine + a fatty acid
-
-
-
?
3.1.1.5
lysophosphatidylethanolamine + H2O
-
678790
Cyberlindnera jadinii
fatty acid + glycerylphosphoethanolamine
-
-
-
?
3.1.1.5
phosphatidic acid + H2O
-
678790
Cyberlindnera jadinii
?
-
-
-
?
3.1.1.5
phosphatidylcholine + H2O
-
678790
Cyberlindnera jadinii
lysophosphatidylcholine + glycerophosphorylcholine + a fatty acid
-
-
-
?
3.1.1.5
phosphatidylethanolamine + H2O
-
678790
Cyberlindnera jadinii
lysophosphatidylethanolamine + glycerophosphorylethanolamine + a fatty acid
-
-
-
?
3.1.1.5
phosphatidylinositol + H2O
-
678790
Cyberlindnera jadinii
lysophophatidylinositol + a carboxylate
-
-
-
?
3.1.1.5
phosphatidylserine + H2O
-
678790
Cyberlindnera jadinii
lysophosphatidylserine + glycerophosphorylserine + a fatty acid
-
-
-
?
Subunits (protein specific)
EC Number
Subunits
Commentary
Organism
3.1.1.5
?
x * 90000-110000, SDS-PAGE
Cyberlindnera jadinii
Temperature Optimum [°C] (protein specific)
EC Number
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
3.1.1.5
40
-
at pH 3.0
Cyberlindnera jadinii
Temperature Stability [°C] (protein specific)
EC Number
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
3.1.1.5
80
-
complete loss of activity after incubation above 80°C
Cyberlindnera jadinii
pH Optimum (protein specific)
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
3.1.1.5
3
-
two pH-optima: pH 3.0 and pH 7.5
Cyberlindnera jadinii
3.1.1.5
7.5
-
two pH-optima: pH 3.0 and pH 7.5
Cyberlindnera jadinii