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Literature summary extracted from

  • Wongchawalit, J.; Yamamoto, T.; Nakai, H.; Kim, Y.M.; Sato, N.; Nishimoto, M.; Okuyama, M.; Mori, H.; Saji, O.; Chanchao, C.; Wongsiri, S.; Surarit, R.; Svasti, J.; Chiba, S.; Kimura, A.
    Purification and characterization of alpha-glucosidase I from Japanese honeybee (Apis cerana japonica) and molecular cloning of its cDNA (2006), Biosci. Biotechnol. Biochem., 70, 2889-2898.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.2.1.20
-
 Apis cerana japonica
3.2.1.20 DNA and amino acid sequence determination and anaylsis Apis cerana japonica

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.2.1.20 additional information
-
 additional information alpha-glucosidase I shows negative cooperative behaviour with substrates sucrose, maltose, and 4-nitrophenyl-alpha-D-glucopyranoside, but positive cooperativity with substrate turanose Apis cerana japonica
3.2.1.20 0.21
-
 p-nitrophenyl alpha-D-glucoside
-
 Apis cerana japonica
3.2.1.20 0.21
-
 4-nitrophenyl-alpha-D-glucopyranoside pH 5.0, 37°C, alpha-glucosidase I Apis cerana japonica
3.2.1.20 0.4
-
 2-((2-(guanin-9-yl)ethyl)(2-((2-hydroxyethyl)((2-phosphonoethyl)amino)ethyl)amino)ethyl)phosphonic acid
-
 Apis cerana japonica
3.2.1.20 0.4
-
 maltotriose pH 5.0, 37°C, alpha-glucosidase I Apis cerana japonica
3.2.1.20 1.3
-
 maltotetraose
-
 Apis cerana japonica
3.2.1.20 1.3
-
 maltotetraose pH 5.0, 37°C, alpha-glucosidase I Apis cerana japonica
3.2.1.20 1.5
-
 maltose
-
 Apis cerana japonica
3.2.1.20 1.5
-
 maltose pH 5.0, 37°C, alpha-glucosidase I Apis cerana japonica
3.2.1.20 2.4
-
 sucrose
-
 Apis cerana japonica
3.2.1.20 5.5
-
 maltopentaose
-
 Apis cerana japonica
3.2.1.20 5.5
-
 maltopentaose pH 5.0, 37°C, alpha-glucosidase I Apis cerana japonica
3.2.1.20 8.7
-
 kojibiose
-
 Apis cerana japonica
3.2.1.20 8.7
-
 kojibiose pH 5.0, 37°C, alpha-glucosidase I Apis cerana japonica

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.1.20 66515
-
 1 * 82000, SDS-PAGE, 1 * 74000, SDS-PAGE, deglycosylated enzyme, 1 * 66515, sequence analysis Apis cerana japonica
3.2.1.20 74000
-
 1 * 82000, SDS-PAGE, 1 * 74000, SDS-PAGE, deglycosylated enzyme, 1 * 66515, sequence analysis Apis cerana japonica
3.2.1.20 82000
-
 alpha-glucosidase I, native PAGE Apis cerana japonica

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.20 Apis cerana japonica A1IHL0
-
-
3.2.1.20 Apis cerana japonica A1IHL0 alpha-glucosidase I; Japanese honeybee
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
3.2.1.20 glycoprotein alpha-glucosidase I contains 15% carbohydrate Apis cerana japonica

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.20 by salting-out chromatography and gel filtration Apis cerana japonica
3.2.1.20 native alpha-glucosidase I by ammonium sulfate fractionation, ion exchange and hydrophobic interaction chromatography, and gel filtration Apis cerana japonica

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.2.1.20 0.13
-
 crude extract Apis cerana japonica
3.2.1.20 13
-
 purified enzyme Apis cerana japonica
3.2.1.20 13
-
 purified alpha-glucosidase I Apis cerana japonica

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.20 4-nitrophenyl alpha-D-glucopyranoside + H2O
-
 Apis cerana japonica 4-nitrophenol + alpha-D-glucose
-
 ?
3.2.1.20 4-nitrophenyl alpha-D-glucoside + H2O
-
 Apis cerana japonica 4-nitrophenol + D-glucose
-
 ?
3.2.1.20 kojibiose + H2O
-
 Apis cerana japonica alpha-D-glucose + D-glucose
-
 ?
3.2.1.20 kojibiose + H2O
-
 Apis cerana japonica ?
-
 ?
3.2.1.20 maltopentaose + H2O
-
 Apis cerana japonica ?
-
 ?
3.2.1.20 maltopentaose + H2O
-
 Apis cerana japonica alpha-D-glucose + ?
-
 ?
3.2.1.20 maltose + H2O
-
 Apis cerana japonica ?
-
 ?
3.2.1.20 maltose + H2O
-
 Apis cerana japonica alpha-D-glucose + D-glucose
-
 ?
3.2.1.20 maltotetraose + H2O
-
 Apis cerana japonica ?
-
 ?
3.2.1.20 maltotetraose + H2O
-
 Apis cerana japonica alpha-D-glucose
-
 ?
3.2.1.20 maltotriose + H2O
-
 Apis cerana japonica ?
-
 ?
3.2.1.20 maltotriose + H2O
-
 Apis cerana japonica maltose + alpha-D-glucose
-
 ?
3.2.1.20 additional information no activity toward isomaltose, nigerose or soluble starch Apis cerana japonica ?
-
 ?
3.2.1.20 additional information substrate specificity, overview, the enzyme is also active with sucrose and turanose Apis cerana japonica ?
-
 ?
3.2.1.20 sucrose + H2O
-
 Apis cerana japonica D-fructose + D-glucose
-
 ?

Subunits

EC Number Subunits Comment Organism
3.2.1.20 monomer 1 * 82000, alpha-glucosidase I, SDS-PAGE Apis cerana japonica
3.2.1.20 monomer 1 * 82000, SDS-PAGE, 1 * 74000, SDS-PAGE, deglycosylated enzyme, 1 * 66515, sequence analysis Apis cerana japonica

Synonyms

EC Number Synonyms Comment Organism
3.2.1.20 alpha-glucosidase I
-
 Apis cerana japonica
3.2.1.20 alpha-glucoside glucohydrolase
-
 Apis cerana japonica
3.2.1.20 JHGase I
-
 Apis cerana japonica
3.2.1.20 maltase
-
 Apis cerana japonica
3.2.1.20 More the enzyme belongs to the alpha-amylase enzyme family Apis cerana japonica

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.20 37
-
 assay at Apis cerana japonica

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.2.1.20 40
-
-
 Apis cerana japonica
3.2.1.20 40
-
 purified alpha-glucosidase I, 15 min, stable up to Apis cerana japonica

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.2.1.20 8.7
-
 kojibiose
-
 Apis cerana japonica
3.2.1.20 8.7
-
 kojibiose pH 5.0, 37°C, alpha-glucosidase I Apis cerana japonica
3.2.1.20 13
-
 maltotetraose
-
 Apis cerana japonica
3.2.1.20 13
-
 p-nitrophenyl alpha-D-glucoside
-
 Apis cerana japonica
3.2.1.20 13
-
 maltotriose pH 5.0, 37°C, alpha-glucosidase I Apis cerana japonica
3.2.1.20 13
-
 maltopentaose pH 5.0, 37°C, alpha-glucosidase I Apis cerana japonica
3.2.1.20 13
-
 4-nitrophenyl-alpha-D-glucopyranoside pH 5.0, 37°C, alpha-glucosidase I Apis cerana japonica
3.2.1.20 15
-
 maltotriose
-
 Apis cerana japonica
3.2.1.20 15
-
 maltopentaose
-
 Apis cerana japonica
3.2.1.20 15
-
 maltotetraose pH 5.0, 37°C, alpha-glucosidase I Apis cerana japonica
3.2.1.20 22
-
 maltose
-
 Apis cerana japonica
3.2.1.20 22
-
 maltose pH 5.0, 37°C, alpha-glucosidase I Apis cerana japonica
3.2.1.20 40
-
 sucrose
-
 Apis cerana japonica

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.20 5
-
-
 Apis cerana japonica
3.2.1.20 5
-
 alpha-glucosidase I Apis cerana japonica

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
3.2.1.20 4.5 10.5
-
 Apis cerana japonica
3.2.1.20 4.5 10.5 purified alpha-glucosidase I, 24 h, 4°C, stable within this range Apis cerana japonica